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Safety and efficacy of an E2 glycoprotein subunit vaccine produced in mammalian cells to prevent experimental infection with bovine viral diarrhoea virus in cattle

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An Erratum to this article was published on 07 July 2016

Abstract

Bovine viral diarrhea (BVD) infection caused by bovine viral diarrhea virus (BVDV), a Pestivirus of the Flaviviridae family, is an important cause of morbidity, mortality and economical losses in cattle worldwide. E2 protein is the major glycoprotein of BVDV envelope and the main target for neutralising antibodies (NAbs). Different studies on protection against BVDV infection have focused on E2, supporting its putative use in subunit vaccines. A truncated version of type 1a BVDV E2 (tE2) expressed in mammalian cells was used to formulate an experimental oleous monovalent vaccine. Immunogenicity was studied through immunisation of guinea pigs and followed by trials in cattle. Calves of 8–12 months were vaccinated, twice with a 4 week interval, with either a tE2 subunit vaccine (n = 8), a whole virus inactivated vaccine (n = 8) or left untreated as negative control group (n = 8). Four weeks after the last immunisation the animals were experimentally challenged intranasally with a non-cythopathic BVDV strain. Following challenge, BVDV was isolated from all unvaccinated animals, while 6 out of 8 animals vaccinated with tE2 showed complete virological protection indicating that the tE2 vaccine presented a similar performance to a satisfactory whole virus inactivated vaccine.

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Acknowledgments

This work was financially supported by Biogénesis-Bagó S.A Argentina. The authors are grateful to Vet Gustavo Combessies (Laboratorio Diagnostico Azul S. A.) for assistance in clinical trials and to Ayelén Sammarruco for technical laboratory assistance.

Ethical standards

The text must contain a declaration that the experiment comply with the current laws of the country in which they were performed.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Instituto de Virología, CICVyA, INTA-Castelar, Buenos Aires, Argentina

    Andrea Pecora, María Sol Pérez Aguirreburualde, Sebastián Chiavenna, Maria J. Dus Santos & Andrés Wigdorovitz

  2. Biogénesis-Bago SA, Buenos Aires, Argentina

    Alejandra Aguirreburualde, Diego Bochoeyer, Marcelo Spitteler, Jorge L. Filippi & Susana M. Levy

  3. EEA Balcarce INTA, Balcarce, Argentina

    Maria Rosa Leunda & Anselmo Odeon

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  1. Andrea Pecora
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  2. María Sol Pérez Aguirreburualde
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  3. Alejandra Aguirreburualde
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  4. Maria Rosa Leunda
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  5. Anselmo Odeon
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  6. Sebastián Chiavenna
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  7. Diego Bochoeyer
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  8. Marcelo Spitteler
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  9. Jorge L. Filippi
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  10. Maria J. Dus Santos
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  11. Susana M. Levy
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  12. Andrés Wigdorovitz
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Corresponding author

Correspondence to Andrés Wigdorovitz.

Additional information

An erratum to this article is available at http://dx.doi.org/10.1007/s11259-016-9660-y.

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Pecora, A., Aguirreburualde, M.S.P., Aguirreburualde, A. et al. Safety and efficacy of an E2 glycoprotein subunit vaccine produced in mammalian cells to prevent experimental infection with bovine viral diarrhoea virus in cattle. Vet Res Commun 36, 157–164 (2012). https://doi.org/10.1007/s11259-012-9526-x

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  • DOI: https://doi.org/10.1007/s11259-012-9526-x

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