Abstract
Estimates of soil seed banks are important to many ecological investigations and plant conservation, yet seed banks are among the most difficult plant community attributes to accurately quantify. To compare extraction and emergence seed bank characterization methods, we collected 0- to 5-cm soil seed bank samples and measured plant community composition in six microsite types (below different perennial plant species and interspaces) at 10 field sites in the Mojave Desert, USA. Extraction detected five times more species sample−1 and orders of magnitude greater seed density than emergence, though evaluating viability of extracted seed was not straightforward. Only 13 % of 847 tested seeds from extraction emerged in follow-up assays. Considering all sites, species detection was more similar between methods: 21 taxa for emergence and 28 for extraction. Results suggest that: (i) capturing microsite variation is critical for efficiently estimating site-level desert seed banks; (ii) method comparisons hinged on the scale of analysis for species richness, as differences in species detection between methods diminished when increasing resolution from the sample to the regional scale; (iii) combining data from all seed bank methods provided the strongest correlation with vegetation; and (iv) improving knowledge of seed germinability is important for advancing both seed bank methods, including for extraction to evaluate the proportion of extracted seeds that are viable. Multifactor approaches that balance several effectiveness measures (e.g., both seed density and species detection at multiple scales) and procedural challenges are most likely to accurately represent complexity in tradeoffs for choosing methods to quantify soil seed banks.
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References
Abella SR, Springer JD, Covington WW (2007) Seed banks of an Arizona Pinus ponderosa landscape: responses to environmental gradients and fire cues. Can J For Res 37:552–567
Abella SR, Engel EC, Lund CL, Spencer JE (2009) Early post-fire plant establishment on a Mojave Desert burn. Madroño 56:137–148
Anderson MJ (2001) A new method for non-parametric multivariate analysis of variance. Aust Ecol 26:32–46
Bakker JP, Poschlod P, Strykstra RJ, Bekker RM, Thompson K (1996) Seed banks and seed dispersal: important topics in restoration ecology. Acta Bot Neerl 45:461–490
Baldwin BG, Boyd S, Ertter BJ, Patterson RW, Rosatti TJ, Wilken DH (2002) The Jepson desert manual: vascular plants of southeastern California. University of California Press, Berkeley
Baskin CC, Baskin JM (1998) Seeds: ecology, biogeography, and evolution of dormancy and germination. Academic, New York
Beatley JC (1974) Phenological events and their environmental triggers in Mojave Desert ecosystems. Ecology 55:856–863
Bernhardt KG, Koch M, Kropf M, Ulbel E, Webhofer J (2008) Comparison of two methods characterising the seed bank of amphibious plants in submerged sediments. Aquat Bot 88:171–177
Cardina J, Sparrow DH (1996) A comparison of methods to predict weed seedling populations from the soil seedbank. Weed Sci 44:46–51
Damschen EI, Harrison S, Ackerly DD, Fernandez-Going BM, Anacker BL (2012) Endemic plant communities on special soils: early victims or hardy survivors of climate change? J Ecol 100:1122–1130
Darwin C (1859) On the origin of species by means of natural selection, or the preservation of favoured races in the struggle for life. John Murray, London
DeFalco LA, Esque TC, Kane JM, Nicklas MB (2009) Seed banks in a degraded desert shrubland: influence of soil surface condition and harvester ant activity on seed abundance. J Arid Environ 73:885–893
Espeland EK, Perkins LB, Leger EA (2010) Comparison of seed bank estimation techniques using six weed species in two soil types. Rangel Ecol Manag 63:243–247
Esque TC, Young JA, Tracy CR (2010) Short-term effects of experimental fires on a Mojave Desert seed bank. J Arid Environ 74:1302–1308
Forcella F (1992) Prediction of weed seedling densities from buried seed reserves. Weed Res 32:29–38
Gross KL (1990) A comparison of methods for estimating seed numbers in the soil. J Ecol 78:1079–1093
Guo Q, Rundel PW, Goodall DW (1998) Horizontal and vertical distribution of desert seed banks: patterns, causes, and implications. J Arid Environ 38:465–478
Ishikawa-Goto M, Tsuyuzaki S (2004) Methods of estimating seed banks with reference to long-term seed burial. J Plant Res 117:245–248
Lato LJ (2006) Soil survey of Clark County area, Nevada. United States Dept Agric, Natural Resources Conserv Serv. Govt Print Office, Washington, DC
Mantel N (1967) The detection of disease clustering and generalized regression approach. Cancer Res 27:209–220
McCune B, Mefford MJ (1999) PC-ORD: multivariate analysis of ecological data. User’s guide. MjM Software Design, Gleneden Beach, OR, USA
Megill L, Walker LR, Vanier C, Johnson D (2011) Seed bank dynamics and habitat indicators of Arctomecon californica, a rare plant in a fragmented desert environment. West North Am Nat 71:195–205
Meyer SE (1986) The ecology of gypsophile endemism in the eastern Mojave Desert. Ecology 67:1303–1313
Natural Resources Conservation Service [NRCS] (2013) The PLANTS database, National Plant Data Center, Baton Rouge. http://plants.usda.gov. Accessed 1 Mar 2013
Nelson JF, Chew RM (1977) Factors affecting seed reserves in the soil of a Mojave Desert ecosystem, Rock Valley, Nye County, Nevada. Am Midl Nat 97:300–320
Olano JM, Caballero I, Loidi J, Escudero A (2005) Prediction of plant cover from seed bank analysis in a semi-arid plant community on gypsum. J Veg Sci 16:215–222
Pake CE, Venable DL (1996) Seed banks in desert annuals: implications for persistence and coexistence in variable environments. Ecology 77:1427–1435
Peet RK, Wentworth TR, White PS (1998) A flexible, multipurpose method for recording vegetation composition and structure. Castanea 63:262–274
Price JN, Wright BR, Gross CL, Whalley WRDB (2010) Comparison of seedling emergence and seed extraction techniques for estimating the composition of soil seed banks. Methods Ecol Evol 1:151–157
Reichman OJ (1984) Spatial and temporal variation of seed distributions in Sonoran Desert soils. J Biogeogr 11:1–11
Richardson PJ, MacDougall AS, Larson DW (2012) Fine-scale spatial heterogeneity and incoming seed diversity additively determine plant establishment. J Ecol 100:939–949
Roberts HA (1981) Seed banks in soils. Adv Appl Biol 6:1–55
Robertson SG, Hickman KR (2012) Aboveground plant community and seed bank composition along an invasion gradient. Plant Ecol 213:1461–1475
SAS Institute (2009) SAS/STAT 9.2 user’s guide. SAS Institute, Cary, NC, USA
Schneider HE, Allen EB (2012) Effects of elevated nitrogen and exotic plant invasion on soil seed bank composition in Joshua Tree National Park. Plant Ecol 213:1277–1287
Thompson K, Band SR, Hodgson JG (1993) Seed size and shape predict persistence in soil. Funct Ecol 7:236–241
Thompson K, Bakker J, Bekker R (1997) The soil seed banks of North West Europe. Cambridge University Press, Cambridge
van der Valk AG, Pederson RL (1989) Seed banks and the management and restoration of natural vegetation. In: Leck MA, Parker VT, Simpson RL (eds) Ecology of soil seed banks. Academic, New York, pp 329–346
Warr SJ, Thompson K, Kent M (1993) Seed banks as a neglected area of biogeographic research: a review of literature and sampling techniques. Prog Phys Geog 17:329–347
Acknowledgments
This study was funded through a cooperative agreement between the National Park Service (Lake Mead National Recreation Area, in particular Alice Newton and Kent Turner) and the University of Nevada Las Vegas (UNLV). We thank Dianne Bangle and Jessica Spencer for help collecting samples at two sites, the Oregon State University Seed Laboratory (Corvallis, OR) for extracting samples, the UNLV research greenhouse for providing space, Kathryn Prengaman and Wes Niles (UNLV) for help with seed identification, Sharon Altman and Cayenne Engel (UNLV) for help preparing figures, and two anonymous reviewers for helpful comments on the manuscript. Any use of trade names is for descriptive purposes only and does not imply endorsement by the US Government.
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Abella, S.R., Chiquoine, L.P. & Vanier, C.H. Characterizing soil seed banks and relationships to plant communities. Plant Ecol 214, 703–715 (2013). https://doi.org/10.1007/s11258-013-0200-3
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DOI: https://doi.org/10.1007/s11258-013-0200-3