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Preparation of PCL/silk fibroin/collagen electrospun fiber for urethral reconstruction

  • Urology - Original Paper
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Abstract

Purpose

To prepare polycaprolactone (PCL)/silk fibroin/collagen electrospun nanofiber scaffold and test its effects on growth and proliferation of oral mucosal epithelial cells.

Methods

Regenerated silk fibroin film, water-soluble collagen powder, and PCL, at mass ratios of 1:1:4, 1:1:8, and 1:1:10, were dissolved in hexafluoroisopropanol, and electrostatic spinning method was adopted to prepare PCL/silk fibroin/collagen electrospun nanofiber scaffold. In vitro cultured oral mucosal epithelial cells were inoculated on the material surface, MTT assay and scanning electron microscopy were adopted to study the growth and proliferation of oral mucosal epithelial cells on the material surface, and cell compatibility of PCL/silk fibroin/collagen electrospun nanofiber was evaluated.

Results

The result of MTT assay showed that oral mucosal epithelial cells were growing well on the PCL/silk fibroin/collagen electrospun nanofiber scaffold. Scanning electron microscopy showed that the prepared electrospun fiber was uniform in diameter and presented an interconnected porous net structure, and oral mucosal epithelial cells had a good growth form on the surface of the modified material.

Conclusions

PCL/silk fibroin/collagen electrospun nanofiber scaffold has appropriate pore size and porosity, is suitable for the growth of oral mucosal epithelial cells, has good cell compatibility, and is a good scaffold for tissue engineering urethral reconstruction.

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References

  1. Mathur RK, Nagar M, Mathur R, Khan F, Deshmukh C, Guru N (2014) Single-stage preputial skin flap urethroplasty for long-segment urethral strictures: evaluation and determinants of success. BJU Int 113(1):120–126. doi:10.1111/bju.12361

    Article  PubMed  Google Scholar 

  2. Kozinn SI, Harty NJ, Zinman L, Buckley JC (2013) Management of complex anterior urethral strictures with multistage buccal mucosa graft reconstruction. Urology 82(3):718–722. doi:10.1016/j.urology.2013.03.081

    Article  PubMed  Google Scholar 

  3. Xu YM, Sa YL, Fu Q, Zhang J, Xie H, Feng C (2013) A rationale for procedure selection to repair female urethral stricture associated with urethrovaginal fistulas. J Urol 189(1):176–181. doi:10.1016/j.juro.2012.09.005

    Article  PubMed  Google Scholar 

  4. Cui T, Terlecki R, Atala A (2014) Tissue engineering in urethral reconstruction. Arch Esp Urol 67(1):29–34

    PubMed  Google Scholar 

  5. Li C, Xu YM, Song LJ, Fu Q, Cui L, Yin S (2008) Urethral reconstruction using oral keratinocyte seeded bladder acellular matrix grafts. J Urol 180(4):1538–1542. doi:10.1016/j.juro.2008.06.013

    Article  PubMed  Google Scholar 

  6. Waxman SW, Morey AF (2006) Management of urethral strictures. Lancet 367(9520):1379–1380. doi:10.1016/s0140-6736(06)68592-9

    Article  PubMed  Google Scholar 

  7. Xu YM, Qiao Y, Sa YL, Wu DL, Zhang J, Zhang XR, Chen R, Si JM (2004) 1-Stage urethral reconstruction using colonic mucosa graft for the treatment of a long complex urethral stricture. J Urol 171(1):220–223; discussion 223. doi:10.1097/01.ju.0000094810.60093.bc

  8. Xu YM, Qiao Y, Sa YL, Wu DL, Zhang XR, Zhang J, Gu BJ, Jin SB (2007) Substitution urethroplasty of complex and long-segment urethral strictures: a rationale for procedure selection. Eur Urol 51(4):1093–1098; discussion 1098–1099. doi:10.1016/j.eururo.2006.11.039

  9. Kessler TM, Schreiter F, Kralidis G, Heitz M, Olianas R, Fisch M (2003) Long-term results of surgery for urethral stricture: a statistical analysis. J Urol 170(3):840–844. doi:10.1097/01.ju.0000080842.99332.94

    Article  PubMed  Google Scholar 

  10. Atala A (2006) Recent applications of regenerative medicine to urologic structures and related tissues. Curr Opin Urol 16(4):305–309. doi:10.1097/01.mou.0000232055.20084.f6

    Article  PubMed  Google Scholar 

  11. Li C, Xu Y, Song L, Fu Q, Cui L, Yin S (2008) Preliminary experimental study of tissue-engineered urethral reconstruction using oral keratinocytes seeded on BAMG. Urol Int 81(3):290–295. doi:10.1159/000151406

    Article  PubMed  Google Scholar 

  12. Ma Z, Kotaki M, Inai R, Ramakrishna S (2005) Potential of nanofiber matrix as tissue-engineering scaffolds. Tissue Eng 11(1–2):101–109. doi:10.1089/ten.2005.11.101

    Article  PubMed  Google Scholar 

  13. Courtney T, Sacks MS, Stankus J, Guan J, Wagner WR (2006) Design and analysis of tissue engineering scaffolds that mimic soft tissue mechanical anisotropy. Biomaterials 27(19):3631–3638. doi:10.1016/j.biomaterials.2006.02.024

    CAS  PubMed  Google Scholar 

  14. Williamson MR, Black R, Kielty C (2006) PCL-PU composite vascular scaffold production for vascular tissue engineering: attachment, proliferation and bioactivity of human vascular endothelial cells. Biomaterials 27(19):3608–3616. doi:10.1016/j.biomaterials.2006.02.025

    CAS  PubMed  Google Scholar 

  15. Jeong L, Yeo IS, Kim HN, Yoon YI, da Jang H, Jung SY, Min BM, Park WH (2009) Plasma-treated silk fibroin nanofibers for skin regeneration. Int J Biol Macromol 44(3):222–228. doi:10.1016/j.ijbiomac.2008.12.008

    Article  CAS  PubMed  Google Scholar 

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Acknowledgments

This study was supported by the National Natural Science Foundation of China (Grant No. 81370793).

Conflict of interest

All authors declare that they have no any conflict of interests.

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Authors and Affiliations

  1. Department of Urology, Affiliated Sixth People’s Hospital, Shanghai Jiaotong University, 600 Yishan Road, Shanghai, 200233, China

    Gaijie Wei, Chao Li, Qiang Fu, Yuemin Xu & Hongbin Li

Authors
  1. Gaijie Wei
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  2. Chao Li
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  3. Qiang Fu
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  4. Yuemin Xu
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  5. Hongbin Li
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Corresponding author

Correspondence to Chao Li.

Additional information

Gaijie Wei and Chao Li have contributed equally to this study.

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Wei, G., Li, C., Fu, Q. et al. Preparation of PCL/silk fibroin/collagen electrospun fiber for urethral reconstruction. Int Urol Nephrol 47, 95–99 (2015). https://doi.org/10.1007/s11255-014-0854-3

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  • DOI: https://doi.org/10.1007/s11255-014-0854-3

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