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Physiological effects of constitutive expression of Oilseed Rape Mosaic Tobamovirus (ORMV) movement protein in Arabidopsis thaliana

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Abstract

Movement proteins (MPs) are non-cell autonomous viral-encoded proteins that assist viruses in their cell-to-cell movement. The MP encoded by Tobamoviruses is the best characterized example among MPs of non-tubule-inducing plant RNA viruses. The MP of Oilseed Rape Mosaic Tobamovirus (ORMV) was transgenically expressed in Arabidopsis thaliana, ecotype RLD, under the expression of the 35S promoter from Cauliflower Mosaic Virus. Transgenic lines were obtained in sense and antisense orientations. One of the sense transgenic lines was further characterized turning out to carry one copy of the transgene inserted in the terminal region of the right arm of chromosome 1. The constitutive expression of ORMV-MP induced mild physiological effects in Arabidopsis. Plants of the transgenic line allowed a faster systemic movement of the phloem tracer carboxyfluorescein. The tracer was unloaded differentially in different flower parts, revealing differential effects of ORMV-MP on phloem unloading in sink organs. On the other hand, transgenic Arabidopsis did not show any effect on biomass partitioning or sugar availability, effects reported for equivalent transgenic solanaceous plants expressing the MP of Tobacco Mosaic Virus, another Tobamovirus. Finally, the transgenic Arabidopsis plants were susceptible to ORMV infection, although showing milder overall symptoms than non-transgenic controls. The results highlight the relevance of the specific host-virus system, in the physiological outcome of the molecular interactions established by MPs.

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Acknowledgements

This work was funded by grants BIO95-0766 and BIO2002-02191 of the Spanish National R+D Plan. CM and IA were the holders of predoctoral fellowships from INIA and the Autonomous Community of Madrid, respectively. We thank Margarita Calvo for her excellent technical support.

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  1. Departamento de Biotecnología, INIA, Autopista A-6 km 7, 28040, Madrid, Spain

    Carmen Mansilla, Isabel Aguilar, David Martínez-Herrera, Flora Sánchez & Fernando Ponz

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  1. Carmen Mansilla
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  2. Isabel Aguilar
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  3. David Martínez-Herrera
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  4. Flora Sánchez
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  5. Fernando Ponz
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Correspondence to Fernando Ponz.

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C. Mansilla and I. Aguilar contributed equally.

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Mansilla, C., Aguilar, I., Martínez-Herrera, D. et al. Physiological effects of constitutive expression of Oilseed Rape Mosaic Tobamovirus (ORMV) movement protein in Arabidopsis thaliana . Transgenic Res 15, 761–770 (2006). https://doi.org/10.1007/s11248-006-9017-3

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