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Improved Carica papaya Tolerance to Carmine Spider Mite by the Expression of Manduca sexta chitinase Transgene

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Abstract

Papaya plants producing the tobacco hornworm (Manduca sexta) chitinase protein were obtained following microprojectile bombardment of embryogenic calli derived from the hypocotyls of the cultivar Kapoho. Polymerase chain reaction (PCR) was carried out to confirm the presence of the transgene. RT-PCR and a quantitative chitinase assay showed increased levels of chitinase activity in every selected transgenic line. Insect bioassays in the laboratory showed that plants expressing the Manduca sexta chitinase gene significantly inhibited multiplication of carmine spider mites (Tetranychus cinnabarinus Boisd.). Experiments conducted to evaluate reaction of the transgenic plants to natural infection by carmine spider mites showed that the Manduca sexta chitinase gene provided increased tolerance under field conditions.

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Abbreviations

2,4-D :

2,4-Dichlorophenoxyacetic acid

BA :

benzyladenine

IBA :

indolebutyric acid

MSCH :

Manduca sexta chitinase

NAA :

naphthaleneacetic acid

NPTII :

neomycin phosphotransferase

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Authors and Affiliations

  1. Hawaii Agriculture Research Center, 99-193 Aiea Heights Drive, Aiea, HI, 96701, USA

    Heather R. K. McCafferty & Yun J. Zhu

  2. USDA ARS, PBARC, 99-193 Aiea Heights Drive, Suite 300, Aiea, HI, 96701, USA

    Paul H. Moore

Authors
  1. Heather R. K. McCafferty
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  2. Paul H. Moore
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  3. Yun J. Zhu
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Correspondence to Yun J. Zhu.

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McCafferty, H.R.K., Moore, P.H. & Zhu, Y.J. Improved Carica papaya Tolerance to Carmine Spider Mite by the Expression of Manduca sexta chitinase Transgene. Transgenic Res 15, 337–347 (2006). https://doi.org/10.1007/s11248-006-0005-4

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  • DOI: https://doi.org/10.1007/s11248-006-0005-4

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