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In vitro tolerance of citrus rootstocks under saline stress

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Abstract

The search for citrus rootstocks that confer resistance and tolerance to biotic and abiotic factors is increasing. Among abiotic stresses, salinity is one of the main factors that significantly affect growth and development in this crop. Faced this situation, genetic improvement seeks, among other aspects, to generate rootstocks that are tolerant to salinity. In this sense, tissue culture constitutes an important tool for citrus improvement, as it helps in the pre-selection of genotypes that best express salinity tolerance. Thus, this work aimed to study the tolerance of 11 citrus rootstocks to saline stress in vitro, using different concentrations of sodium chloride (NaCl). For analysis of in vitro regeneration, 1 cm long stem apexes, originating from the in vitro cultivation of rootstocks, were introduced in basal WPM medium and supplemented with 0.64, 1.28, 1.92, 2.56 and 3.20 g L−1 of NaCl. After 120 days in a growth room, the plants were evaluated and the data were analyzed using the R statistical program. The genotypes showed different behaviors depending on the interaction between the studied factors. There was a reduction in most of the in vitro development variables of rootstocks grown in culture medium supplemented with NaCl. The ‘Riverside’ citrandarin genotypes, HTR 051, LRF (LCR × TR) - 005, BRS Bravo, TSK × TRBK - Colômbia and BRS H Montenegro, were more tolerant to salinity, while TSKC × CTSW - 028, the mandarin ‘Sunki Tropical’ and BRS Santana were the most sensitive.

Key Message

In this study, we identified genotypes of citrus rootstocks with different levels of salinity tolerance through tissue culture, contributing to genetic improvement and enhancement of citrus production.

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All data generated were analyzed during this study and are included in this published article.

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Acknowledgments

The author expresses her sincere thanks to Dr. Antônio da Silva Souza for his invaluable support, valuable guidance and excellent work, as well as for the fundamental review of the manuscript. Your dedication and contribution were essential to the quality of this work.

Funding

To CAPES and FAPESB, for their financial support for the scholarships of the students involved in the study. To Embrapa Mandiocae Fruticultura, for providing the physical structure and financial.

support for the development of the study.

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Authors and Affiliations

  1. State University of Santa Cruz, Ilhéus, Brazil

    Denise dos Santos Vila Verde & Maria Inês de Souza Mendes

  2. Federal University of Recôncavo da Bahia, Cruz das Almas, Brazil

    Leila Vasconcelos da Costa Nobre

  3. Embrapa Cassava and Fruticulture, Cruz das Almas, Brazil

    Antônio da Silva Souza, Karen Cristina Fialho dos Santos & Walter dos Santos Soares Filho

Authors
  1. Denise dos Santos Vila Verde
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  2. Maria Inês de Souza Mendes
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  3. Leila Vasconcelos da Costa Nobre
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  4. Antônio da Silva Souza
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  5. Karen Cristina Fialho dos Santos
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  6. Walter dos Santos Soares Filho
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Contributions

DSVV, MISM and LVCN: collected the data and interpreted the results. DSVV and MISM: wrote the manuscript. DSVV, MISM, ASS, KCFS and WSSF: provided technical support to set up and conduct the study. ASS, KCFS, and WSSF: defined the main lines of the experiments. DSVV: supported the statistical analysis. ASS, KCFS and WSSF: were the supervisors of this research. ASS, WSSF and KCFS: contributed with the financial acquisition of the experiments.

Corresponding author

Correspondence to Denise dos Santos Vila Verde.

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Conflict of interest

The authors declare that they have no known competing fnancial interests or personal relationships that could have appeared to infuence the work reported in this paper.

Additional information

Communicated by Maria Antonietta Germanà.

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Vila Verde, D.d., Mendes, M.I.d., Nobre, L.V.d. et al. In vitro tolerance of citrus rootstocks under saline stress. Plant Cell Tiss Organ Cult 156, 13 (2024). https://doi.org/10.1007/s11240-023-02627-y

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  • DOI: https://doi.org/10.1007/s11240-023-02627-y

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