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In vitro propagation of Paepalanthus chiquitensis Herzog (Eriocaulaceae), an endangered everlasting flower species

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Abstract

Paepalanthus chiquitensis Herzog (Eriocaulaceae) is a neotropical everlasting marketed internationally in the dry flower segment. In situ exploitation has led to the possibility of extinction, necessitating the identification of alternatives for maintaining its natural populations. In vitro cultivation, a promising biotechnological tool that allows plant regeneration under aseptic conditions, has been explored in several Brazilian ornamental plants. However, no reports on in vitro cultivation of P. chiquitensis have been reported. Here, we aimed to establish an in vitro cultivation protocol for P. chiquitensis from diaspores collected in their natural habitat. Explant disinfection with 0.75% sodium hypochlorite solution reduced the contamination rate and allowed better germination. Although the species developed well in a culture medium without the addition of cytokinins or auxins, supplementation of naphthalene-acetic acid (NAA; 1 mg L− 1) and isopentenyl adenine (1 mg L− 1) and the combination of NAA (0.25 mg L− 1) and activated charcoal (2 g L− 1) promoted shoot and root development, respectively. These results pave the foundation for future studies on in vitro cultivation of P. chiquitensis to mitigate the risk of extinction of everlastings.

Key message

This study is the first report on in vitro propagation of Paepalanthus chiquitensis Herzog.

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References

  • Albuquerque MMS, Brito AL, Lima APPS, Alvim BFM, Santana JRF (2016) In vitro establishment of Comanthera curralensis, “sempre viva” native of Chapada Diamantina - Bahia. Revista Ciência Rural 46:991–995. https://doi.org/10.1590/0103-8478cr20131091

    Article  CAS  Google Scholar 

  • Albuquerque MMS (2013) Micropropagação e conservação in vitro de “sempre-vivas” nativas da Chapada Diamantina-BA. Dissertation, Universidade Estadual de Feira de Santana-UEFS

  • Andrino CO, Costa FN, Sano PT (2015) O gênero Paepalanthus Mart. (Eriocaulaceae) no parque Estadual do Biribiri, Diamantina, Minas Gerais. Brasil Rodriguésia 66:393–419. https://doi.org/10.1590/2175-7860201566209

    Article  Google Scholar 

  • Aoyama EM, Gontijo ABPL, Faria DV (2012) Propagação em Bromeliaceae: germinação de sementes e cultivo in vitro. Revista Enciclopédia Biosfera 8:1452–1471

    Google Scholar 

  • Baker A, Kaviani B, Nematzadeh G, Negahdar N (2014) Micropropagation of Orchis catasetum - a rare and endangered orchid. Acta Scientiarum Polonorum 13:197–205

    Google Scholar 

  • Candido DF (2013) Cultivo in vitro de Peltophorum dubium. (sprengel) taubert: multiplicação,. Senescência foliar e calogênese. Dissertation, Universidade Federal de Santa Maria-UFSM

  • Centellas AQ, Fortes GRL, Müller NTG, Zanol GC, Flores R, Gottinari RA (1999) Efeito de auxinas sintéticas no enraizamento in vitro da Macieira. Pesquisa Agropecuária Brasileira Brasília 34:181–186. https://doi.org/10.1590/S0100-204X1999000200005

    Article  Google Scholar 

  • Cerqueira CO, Funch LS, Borba EL (2008) Fenologia de Syngonanthus mucugensis Giul. subsp. mucugensis e S. curralensis Moldenke (Eriocaulaceae), nos municípios de Mucugê e Morro do Chapéu, Chapada Diamantina, BA, Brasil. Acta Botânica Brasilica 22: 962–969. https://doi.org/10.1590/S0102-33062008000400007

  • Coutinho LM (1990) O cerrado e a ecologia do fogo. Revista Ciência Hoje 12:22–30

    Google Scholar 

  • Coutinho LM (2002) O bioma do cerrado. In: Klein AL (Ed.). Eugen Warming e o cerrado brasileiro: um século depois. São Paulo: Unesp, imprensa Oficial do Estado. pp 77–91

  • Cui Y, Deng Y, Zheng K, Hu X, Zhu M, Deng X, Xi R (2019) An efficient micropropagation protocol for an endangered ornamental tree species (Magnolia sirindhorniae Noot. & Chalermglin) and assessment of genetic uniformity through DNA markers. Sci Rep 9. https://doi.org/10.1038/s41598-019-46050-w

  • Dantas S, Oliveira S, Câmara T (2002) Contaminação microbiana no cultivo in vitro de plantas. In: Luz WC. da (Org.). Revisão Anual de Patologia de Plantas. 10a ed. Passo Fundo, RAPP 10: 391–407

  • Dignart SL (2006) Luz e sacarose na micropropagação de Cattleya walkeriana: alterações anatômicas e fisiológicas. Dissertation, Universidade Federal de Lavras-UFLA

  • Dobrovolski R, Loyola RD, Marco Júnior P, Diniz-Filho JAF (2011) Agricultural expansion can menace brazilian protected areas during the 21st century. Natureza e Conservação 9:208–213. https://doi.org/10.4322/natcon.2011.027

    Article  Google Scholar 

  • Erig AC, Schuch MW, Braga EJB (2004) Enraizamento in vitro de pereira (Pyrus communis L.) cv. Carrick Revista Ciência Rural 34:275–277. https://doi.org/10.1590/S0103-84782004000100043

    Article  Google Scholar 

  • Figueiredo AJR (2017) Enraizamento in vitro de Eucalyptus cloeziana f. Muell. Dissertation, Universidade Federal do Mato Grosso-UFMT

  • Freitas HMV, Trovó M (2017) Eriocaulaceae da região serrana do estado do Rio de Janeiro, Brasil. Revista Rodriguésia 68:749–757. https://doi.org/10.1590/2175-7860201768225

    Article  Google Scholar 

  • Freitas Neto OG (2009) Micropropagação e anatomia foliar de Canela-de-Ema (Vellozia flavicans Mart. ex Schult f.-Velloziaceae) em diferentes condições ambientais. Dissertation, Universidade de Brasília-UnB

  • Gashi B, Abdullai K, Sota V, Kongjika E (2015) Micropropagation and in vitro conservation of the rare and threatened plants Ramonda serbica and Ramonda nathalie. Physiol Mol Biol Plants 21:123–136. https://doi.org/10.1007/s12298-014-0261-3

    Article  PubMed  Google Scholar 

  • Grattapaglia D, Machado MA (1998) Micropropagação. In: Torres AC, Caldas LS, Buso JA (Ed). Cultura de tecidos e transformação genética de plantas. Brasília, DF, Embrapa-SPI/Embrapa-CNPH. pp 183–260

  • Guson RR, Moraes CP, Ronconi CC (2012) Influência de diferentes concentrações de carvão ativado no crescimento e enraizamento in vitro de Cattleya pumila Hook. Revista em Agronegócios. e Meio Ambiente 5:551–563

    Google Scholar 

  • Hise R (1996) Chlorination. In: Dence CW, Reeve DW (Ed). Pulp bleaching: Principles and practice. Atlanta, Georgia-USA, Tappi Press, Seção IV, cap. 2, pp 241–259

  • Instituto Chico Mendes de Conservação da Biodiversidade - ICMBio (2011) Sumário Executivo do Plano de Ação Nacional para Conservação das Sempre-Vivas. 8 p. https://www.gov.br/icmbio/pt-br/assuntos/biodiversidade/pan/pan-sempre-vivas/1-ciclo/pan-sempre-vivas-sumario.pdf. Accessed 14 February 2023

  • Leitzke LN, Damiani CR, Schuch MW (2009) Multiplicação e enraizamento in vitro de amoreira-preta ‘xavante’: efeito da concentração de sais, do tipo de explante e de carvão ativado no meio de cultura. Revista Ciência e Agrotecnologia 33:1959–1966. https://doi.org/10.1590/S1413-70542009000700045

    Article  Google Scholar 

  • Léon EAB (2010) Germinação, estabelecimento, regeneração e calogênese in vitro em explantes de açoita-cavalo (luehea divaricata mart. & zucc.). Dissertation, Universidade Federal de Santa Maria-UFSM

  • Lima-Brito A, Albuquerque MMS, Alvim BFM, Resende SV, Bellintani MC, Santana JRF (2011) Agentes osmóticos e temperatura na conservação in vitro de sempre-viva. Revista Ciência Rural 41:1354–1361. https://doi.org/10.1590/S0103-84782011000800010

    Article  CAS  Google Scholar 

  • Markovic M, Grbic M, Djukic M (2016) Micropropagation of endangered and decorative species Dianthus pinifolius Sibth. et Sm. Brazilian Archives of Biology and Technology 59:16150320. https://doi.org/10.1590/1678-4324-2016150320

  • Miransari M, Smith DL (2014) Plant hormones and seed germination. Environ Exp Bot 99:110–121. https://doi.org/10.1016/j.envexpbot.2013.11.005

    Article  CAS  Google Scholar 

  • Melo JT, Silva JA, Torres RAA, Silveira CES, Caldas LS (1998) Coleta, propagação e desenvolvimento inicial de espécies do cerrado. In: Sano SM, Almeida SP (eds) Cerrado: ambiente e flora. Embrapa, Planaltina-DF, pp 195–246

    Google Scholar 

  • Murashige T, Skoog FA (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Plant Physiol 15:473–479. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x

    Article  CAS  Google Scholar 

  • Nunes UR, Nunes SCP, Fonseca PG, Pego RG (2008) Efeito da época de colheita, irrigação e permanência de sementes em solo seco no desenvolvimento inicial de plântulas de Syngonanthus elegans. (Bong) Ruhland Revista Brasileira de Sementes 30:64–70. https://doi.org/10.1590/S0101-31222008000300009

    Article  Google Scholar 

  • Paixão-Santos J, Dornelles ALC, Silva JRS, Rios AP (2003) Germinação in vitro de Syngonanthus mucugensis Giulietti. Sitientibus série Ciências Biológicas 3: 120–124

  • Pan MJ, Van Staden J (1998) The use of charcoal in in vitro culture–A review. Plant Growth Regulations 26:155–163. https://doi.org/10.1023/A:1006119015972

    Article  CAS  Google Scholar 

  • Pêgo RG, Paiva PDO, Paiva R (2013) Micropropagation of Syngonanthus elegantulus. Ciência e Agrotecnologia 37:32–39. https://doi.org/10.1590/S1413-70542013000100004

    Article  Google Scholar 

  • Pêgo RG, Paiva PDO, Paiva R (2014) Micropropagation Protocol for Syngonanthus elegans (Bong.) Ruhland: An ornamental species. Acta Scientiarum. Agronomy 36: 347–353. https://doi.org/10.4025/actasciagron.v36i3.17946

  • Pio Corrêa M (1984) Dicionário das plantas úteis do Brasil e das exóticas cultivadas. Instituto Brasileiro de Desenvolvimento Florestal. Rio de Janeiro, RJ

  • Philipson WR (1946) Studies in development of the inflorescence, I. The capitulum of Bellis perennis L. Ann Botany 10:257–270. https://doi.org/10.1093/oxfordjournals.aob.a083135

    Article  Google Scholar 

  • Prakash V, Vishwakarma K, Singh VP, Rai P, Ramawat N, Tripathi DK, Sharma S (2020) NO and ROS implications in the organization of root system architecture. Physiol Plant 168:473–489. https://doi.org/10.1111/ppl.13050

    Article  CAS  PubMed  Google Scholar 

  • Prudente DO, Nery FC, Reis MV, Paiva PDO, Nery MC, Amin TO (2015) Germinação in vitro e aclimatização de sempre-viva. Plant Cell Culture and Micropropagation 11:62–69

    Google Scholar 

  • Raizer MDM (2011) Multiplicação in vitro e aclimatização de mudas micropropagadas de Heliconia chartacea (Lane x Barreiros) var. Sexy Pink. Dissertation, Instituto Nacional de Pesquisas da Amazônia-INPA

  • Ranal MA, Santana DG (2006) How and why to measure the germination process? Revista Brasileira de Botânica 29:1–11. https://doi.org/10.1590/S0100-84042006000100002

    Article  Google Scholar 

  • Resende A (2009) Avaliação do hipoclorito de sódio na severidade da ferrugem asiática, do oídio e nas características químicas da soja. Thesis, Universidade de Brasília-UnB

  • Ribeiro MC (1999) Fisiologia do crescimento inicial e do florescimento de Paepalanthus speciosus Koern. Dissertation, Universidade Federal de Goiás-UFG

  • Sá e Carvalho CG, Ribeiro MC (1994a) Efeito do armazenamento e de reguladores de crescimento na germinação de Paepalanthus speciosus, Eriocaulaceae. Revista Brasileira de Botânica 17:61–65

    Google Scholar 

  • Sá e Carvalho CG, Ribeiro MC (1994b) Efeitos de choques térmicos na germinação de Paepalanthus speciosus Koern (Euriocaulaceae). Acta Bot Brasilica 8:205–211. https://doi.org/10.1590/S0102-33061994000200005

    Article  Google Scholar 

  • Santos JP, Dornelles ALC, Silva JRS, Santana JRF, Lima-Brito A (2006) Ajuste do meio MS para o cultivo “in vitro” de Syngonanthus mucugensis giulietti, espécie ameaçada de extinção. Sitientibus Série Ciências Biológicas 6:36–39

    Google Scholar 

  • Santos JP, Dornelles ALC, Pereira FD, Oliveira LM (2008) Indução de calos em sempre-viva (Syngonanthus mucugensis Giulietti), utilizando diferentes tipos de explantes e concentrações de BAP. Acta Scientiarum Biological Sciences 30:127–131. https://doi.org/10.4025/actascibiolsci.v30i2.3624

    Article  CAS  Google Scholar 

  • Scarano FR (2009) Plant communities at the periphery of the Atlantic rainforest: rare-species bias and its risks for conservation. Biol Conserv 142:1201–1208. https://doi.org/10.1016/j.biocon.2009.02.027

    Article  Google Scholar 

  • Silva JRS, Lima-Brito A, Santana JRF, Dornelles ALC (2005a) Efeito da sacarose sobre o enraizamento e desenvolvimento “in vitro” de Syngonanthus mucugensis Giul. Sitientibus Série Ciências Biológicas 5:56–59

    Article  Google Scholar 

  • Silva JRS, Santos JP, Rios APS, Santana JRF, Dornelles ALC (2005b) Estudo da germinação e morfologia do desenvolvimento pós-seminal de Syngonanthus mucugensis Giul. “in vitro”. Sitientibus Série Ciências Biológicas 5:60–62

    Article  Google Scholar 

  • Sofiatti V, Araujo EF, Araujo RF, Reis MS, Silva LVBD, Cargnin A (2008) Uso do hipoclorito de sódio para degradação do endocarpo de sementes de cafeeiro com diferentes graus de umidade. Revista Brasileira de Sementes 30:150–160. https://doi.org/10.1590/S0101-31222008000100019

    Article  Google Scholar 

  • Souto JS, Morimoto JM, Ferreira WM, Nakabashi M, Suzuki RM (2010) Efeitos do ácido naftalenoacético no desenvolvimento in vitro de Cattleya bicolor Lindl. (Orchidaceae). Revista Brasileira de Biociências 8:179–185

    Google Scholar 

  • Tognetti VB, Bielach A, Hrtyan M (2017) Redox regulation at the site of primary growth: Auxin, cytokinin, and ROS crosstalk. Plant Cell & Environment 40:2586–2605. https://doi.org/10.1111/pce.13021

    Article  CAS  Google Scholar 

  • Winhelmann MC, Tedesco M, Lucchese JR, Fior CS, Schafer G (2019) Propagação in vitro de Angelonia integerrima. Revista Rodriguésia 70:1–12. https://doi.org/10.1590/2175-7860201970025

    Article  Google Scholar 

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Acknowledgements

We sincerely acknowledge the editors and peer reviewers whose important criticism improved this manuscript. This research was supported by the Coordination for the Improvement of Higher Education Personnel - CAPES (Financial code 001), National Council for Scientific and Technological Development (CNPq), and Foundation for Research Support of the State of Minas Gerais (FAPEMIG).

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Authors and Affiliations

  1. Institute of Agrarian Sciences, Federal University of Uberlândia-UFU, Avenue Amazonas, s/n, Forest Seed Laboratory, block 4C, chamber 114C, Umuarama, Uberlândia, 38405-302, MG, Brazil

    Carollayne Gonçalves-Magalhães, Denise Garcia de Santana, Simone Abreu Asmar, João Paulo Ribeiro-Oliveira & José Magno Queiroz Luz

  2. Federal Institute of Education, Science and Technology, IFSULDEMINAS, Machado, MG, Brazil

    Tâmara Prado de Morais

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  1. Carollayne Gonçalves-Magalhães
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  2. Tâmara Prado de Morais
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  3. Denise Garcia de Santana
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  4. Simone Abreu Asmar
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  5. João Paulo Ribeiro-Oliveira
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  6. José Magno Queiroz Luz
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Contributions

DS and JL conceived the study and supervised the experiments. CG-M, TM, and SA designed and performed in vitro experiments. CG-M, TM, JR-O, and SA wrote the manuscript. All the authors contributed to the discussion and approved the final version of the manuscript.

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Correspondence to Carollayne Gonçalves-Magalhães.

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Communicated by Maurizio Lambardi.

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Gonçalves-Magalhães, C., de Morais, T.P., de Santana, D.G. et al. In vitro propagation of Paepalanthus chiquitensis Herzog (Eriocaulaceae), an endangered everlasting flower species. Plant Cell Tiss Organ Cult 153, 377–386 (2023). https://doi.org/10.1007/s11240-023-02475-w

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