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A peptide encoding gene MdCLE8 regulates lateral root development in apple

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Abstract

Nitrogen is not only an essential nutrient for plant, but also an important signal molecule to integrate and regulate gene expression, metabolism and growth. Plant peptides are considered as a new type of hormone, and play an important regulatory role in plant growth and development. However, little is known about the co-regulation network between nitrogen and peptide hormones in plant. Here we identified an apple MdCLE8 gene, which encodes a putative peptide, induced by nitrogen deficiency in apple. Ectopic expression of MdCLE8 inhibited lateral root formation in Arabidopsis under nitrogen deficiency. Similarly, overexpression of MdCLE8 inhibited lateral root development in apple adventitious roots, and this inhibition was amplified under nitrogen deficiency treatment. Further studies showed that MdCLE8 may inhibit the expression of several key genes during lateral root emergence stage in Arabidopsis, thereby inhibiting the emergence of lateral root from root cortex cells. Collectively, our study not only broadened the gene regulatory network under the influence of nitrogen in apple, but also expanded the function of CLE peptide hormones in apple.

Key message

The apple peptide encoding gene MdCLE8 is induced by nitrogen deficiency signaling and negatively regulates lateral root formation in plants.

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Data availability

All data generated or analysed during this study are included in this published article [and its supplementary information files].

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Acknowledgements

This work was supported by National Key R&D Program of China (2018YFD1000100); National Natural Science Foundation of China (31772288); Natural Science Foundation of Shandong Province (ZR2020ZD43); Ministry of Agriculture of China (CARS-27).

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Authors and Affiliations

  1. National Key Laboratory of Crop Biology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an, 271018, Shandong, China

    Tian-en Zhang, Yan Shi, Xiu-ming Li & Chun-xiang You

  2. College of Horticulture, Qingdao Agricultural University, Qingdao, 266109, Shandong, China

    Qiang Zhao

Authors
  1. Tian-en Zhang
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  2. Yan Shi
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  3. Xiu-ming Li
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  4. Qiang Zhao
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  5. Chun-xiang You
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Contributions

C-XY, QZ, and T-EZ conceived and designed the experiments. C-XY and QZ supervised the experiments. T-EZ, YS and X-ML performed the experiments. All the authors read and approved the final manuscript.

Corresponding authors

Correspondence to Qiang Zhao or Chun-xiang You.

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The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. The authors have no conflict of interest to declare.

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Communicated by Francisco de Assis Alves Mourão Filho.

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Zhang, Te., Shi, Y., Li, Xm. et al. A peptide encoding gene MdCLE8 regulates lateral root development in apple. Plant Cell Tiss Organ Cult 148, 419–427 (2022). https://doi.org/10.1007/s11240-021-02182-4

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