Abstract
Copper oxide (CuO) nanoparticles (NPs) synthesized through co-precipitation method were employed in MS media during in vitro culture of Stevia rebaudiana. Physiological characteristics, production of steviol glycosides, and antioxidative parameters were investigated in regenerated plants. CuO NPs had crystalline monoclinic cubic cuprous oxides with average size 47 nm. The NPs were applied at 0, 0.1, 1.0, 10, 100 and 1000 mg/L in MS media for direct organogenesis of S. rebaudiana from nodal segments. Shoot organogenesis was found highest (88.5%) at 10 mg/L CuO and average shoot length, mean number of shoot per explant, and fresh weight were also found significantly higher at the same concentration. High performance liquid chromatography (HPLC) illustrated significant rise of bioactive major steviol glycosides (rebaudioside A and stevioside) at 10 mg/L CuO NPs in MS media. The oxidative stress produced by CuO nanoparticles on S. rebaudiana was affirmed by antioxidant activities i.e. total antioxidant activity (TAC), total reducing power (TRP) and 2,2-diphenyl-1-picryl hydrazyl (DPPH)-free radical scavenging activity. The oxidative stress generated by NPs involved production of antioxidative molecules total phenolic content (TPC), total flavonoid content (TFC) depending on NPs concentration. The study concludes that copper oxide nanoparticles functions as a stimulator of bioactive components productions, and can be employed in in vitro batch cultures.
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Acknowledgements
Authors are grateful to The Scientific and Technological Research Council of Turkey, TUBİTAK (Program # 2216) for providing financial support. We are also thankful to the Department of Biology and Seed Science and Technology Abant Izzet Baysal University, Bolu, Turkey; Department of Biotechnology and Department of Physics, Quaid-i-Azam University, Islamabad, Pakistan for providing all the research facilities.
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RJ designed the study and wrote manuscript. AM and RJ performed experimental work. BY and RJ analyzed the data. MZ and EG critically reviewed the manuscript and added to its technical part. All authors have contributed, seen and approved the manuscript.
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Communicated by Silvia Moreno.
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Javed, R., Mohamed, A., Yücesan, B. et al. CuO nanoparticles significantly influence in vitro culture, steviol glycosides, and antioxidant activities of Stevia rebaudiana Bertoni. Plant Cell Tiss Organ Cult 131, 611–620 (2017). https://doi.org/10.1007/s11240-017-1312-6
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DOI: https://doi.org/10.1007/s11240-017-1312-6