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Putrescine promotes changes in the endogenous polyamine levels and proteomic profiles to regulate organogenesis in Cedrela fissilis Vellozo (Meliaceae)

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Abstract

Improvements to in vitro organogenesis are essential for optimizing shoot development and understanding basic physiological processes. The addition of polyamines (PAs) to the culture medium has been used to modulate organogenesis in plants, and this work evaluated the effects of exogenous PAs on direct organogenesis from apical and cotyledonary nodal Cedrela fissilis explants as well as the effects of putrescine (Put) on endogenous PA levels and variations in protein abundance. The effects of exogenous Put, spermidine, and spermine at 0, 0.5, 1, 2.5, or 5 mM on shoot development were tested. The comparison of the tested PAs to the control treatment revealed that 2.5 mM Put significantly increased the length of shoots from cotyledonary nodal explants, which are more sensitive than apical nodal explants, and treatment with 2.5 mM Put significantly increased the endogenous total free-PA and free-Put levels in shoots compared with the control (no Put). A comparative proteomic analysis of shoots indicated that 2.5 mM Put significantly changed the abundance of proteins, primarily metabolic and cellular proteins associated with stress and energy processes such as cell division. These results show that Put functions in endogenous PA metabolism and alters protein abundance, thereby contributing to shoot development in C. fissilis.

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Abbreviations

BA:

6-Benzyladenine

FM:

Fresh matter

IAA:

Indole-3-acetic acid

MS:

Murashige and Skoog

PAs:

Polyamines

PGRs:

Plant growth regulators

Put:

Putrescine

Spd:

Spermidine

Spm:

Spermine

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Acknowledgements

Funding for this work was provided by the National Council for Scientific and Technological Development (CNPq) (444453/2014-8) and the Carlos Chagas Filho Foundation for Research Support in the State of Rio de Janeiro (FAPERJ) (E26/010.001507/2014). VPMA thanks the Coordination for the Improvement of Higher Education Personnel (CAPES) for the scholarship provided, and RSR acknowledges the scholarship funded by FAPERJ.

Author contributions

CSC, VS and VPMA conceived the study, designed the experiments and wrote the manuscript. VPMA was responsible for the in vitro cultures. VPMA and CSC were responsible for the PA experiments and analyses. VPMA, RSR and VS were responsible for the proteomic analyses. All authors read and approved the final manuscript.

Author information

Authors and Affiliations

  1. Laboratório de Biologia Celular e Tecidual (LBCT)-CBB, Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Avenida Alberto Lamego, 2000, Parque Califórnia, Campos dos Goytacazes, RJ, CEP 28013-602, Brazil

    Victor Paulo Mesquita Aragão & Claudete Santa-Catarina

  2. Laboratório de Biotecnologia (LBT)-CBB, UENF, Campos dos Goytacazes, RJ, Brazil

    Ricardo Souza Reis & Vanildo Silveira

  3. Unidade de Biologia Integrativa, Setor de Genômica e Proteômica, UENF, Campos dos Goytacazes, RJ, Brazil

    Ricardo Souza Reis & Vanildo Silveira

Authors
  1. Victor Paulo Mesquita Aragão
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  2. Ricardo Souza Reis
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  3. Vanildo Silveira
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  4. Claudete Santa-Catarina
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Corresponding author

Correspondence to Claudete Santa-Catarina.

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The authors declare that they have no conflict of interest.

Additional information

Communicated by Paula M. Pijut.

Electronic supplementary material

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Supplementary Fig. 1 Phylogenetic placements of Cedrela fissilis and Citrus sinensis. (JPG 328 KB)

11240_2017_1239_MOESM2_ESM.tif

Supplementary Fig. 2 Effects of Put, Spd, and Spm on the induction (a), length (b), and number (c) of shoots from apical nodal explants from Cedrela fissilis after 30 days of culture. Means followed by different letters are significantly different (P < 0.05) according to the SNK test. Capital letters indicate significant differences for a single PA at different concentrations. Lowercase letters indicate significant differences between different PAs at the same concentration. CV = coefficient of variation (n = 8; CV for shoot induction = 15.5%; CV for shoot length = 14.8%; CV for shoot number = 12.5%). (TIF 1708 KB)

Supplementary material 3 (XLSX 118 KB)

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Aragão, V.P.M., Reis, R.S., Silveira, V. et al. Putrescine promotes changes in the endogenous polyamine levels and proteomic profiles to regulate organogenesis in Cedrela fissilis Vellozo (Meliaceae). Plant Cell Tiss Organ Cult 130, 495–505 (2017). https://doi.org/10.1007/s11240-017-1239-y

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