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Modified light spectral conditions prior to cryopreservation alter growth characteristics and cryopreservation success of potato (Solanum tuberosum L.) shoot tips in vitro

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Abstract

Light is one of the most important factors affecting growth and morphogenesis of plants. Light intensity, photoperiod and spectral composition greatly affect morphogenetic responses of in vitro plants. Modification of light spectra during recovery after cryopreservation improves survival and regeneration, but the effect of modified light conditions prior to cryopreservation are not known. Therefore, the aim of the present study was to follow the photomorphogenetic response of potato plants (Solanum tuberosum L.) under different light qualities i.e. cool white fluorescent (CW) used as control, warm white (HQI), white LEDs (W), blue LEDs (B), red LEDs (R) and a combination of red with 10 % of blue LEDs (RB) prior to cryopreservation, affecting recovery of cultivars Agrie Dzeltenie, Bintje, Maret, Anti and Désirée in vitro. Light spectral quality had a significant effect on growth characteristics of potato plants in vitro. Red light (R) promoted elongation growth but biomass accumulation remained low under monochromatic light treatments. Some of the pre-cryopreservation light treatments significantly affected post-cryopreservation success. Under blue LEDs, high early recovery was observed for all cultivars tested, whereas under red (R) or (HQI), lowest survival percentages were obtained 2–4 weeks after thawing. Specifically, during early recovery, blue light increased survival from 26 to 66 %, 4 to 31 % and 16 to 48 % for cultivars Agrie Dzeltenie, Anti, and Désirée, compared to illumination by red LEDs. Therefore, light spectral quality prior to cryopreservation can significantly affect the cryopreservation success of potato shoot tips.

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Abbreviations

B:

Blue LEDs

CW:

Cool white fluorescent tubes

DMSO:

Dimethyl sulfoxide

FW:

Fresh weight

HQI:

Hydrargyrum quartz iodide (warm white)

LEDs:

Light emitting diodes

LN:

Liquid nitrogen

MS:

Murashige and Skoog tissue culture medium (1962)

R:

Red LEDs

PPFD:

Photosynthetic photon flux density

PPM:

Plant preservative mixture

RB:

Combination of red and blue LEDs

W:

White LEDs

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Acknowledgments

This work was funded by national scholarship program Kristjan Jaak (to JE), which is funded and managed by Archimedes Foundation in collaboration with the Estonian Ministry of Education and Research. Additional funding was provided by Tauno Tönning Foundation and Niemi Foundation (to JE). Tarja Törmänen, Matti Rauman and Taina Uusitalo are sincerely acknowledged for technical support in laboratory work.

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Authors and Affiliations

  1. Genetics and Physiology, University of Oulu, PO. Box 3000, 90014, Oulu, Finland

    Jaanika Edesi, Anna Maria Pirttilä & Hely Häggman

Authors
  1. Jaanika Edesi
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  2. Anna Maria Pirttilä
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  3. Hely Häggman
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Correspondence to Jaanika Edesi.

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11240_2016_1119_MOESM1_ESM.pdf

The final status of the cryopreserved potato (Solanum tuberosum L.) shoot tips originating from different pre-cryopreservation light quality treatments CW cool white fluorescent, HQI warm white, W white LEDs, B blue LEDs, R red LEDs, RB combination of 90 % red and 10 % bue LEDs. At the final evaluation of the second repetition of the experiment, the status of the shoot tips was classified as Re regenerating, kRe regenerating with appearance of contamination, g green stagnant shoot tips, kg green shoot tips with contamination, b bown shoot tips, kb brown shoot tips with contamination, kd the shoot tips that turned white soon after thawing and experienced appearance of contamination at the end of the experiment. Each light treatment contained 45 cryopreserved shoot tips (PDF 96 KB)

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Edesi, J., Pirttilä, A.M. & Häggman, H. Modified light spectral conditions prior to cryopreservation alter growth characteristics and cryopreservation success of potato (Solanum tuberosum L.) shoot tips in vitro. Plant Cell Tiss Organ Cult 128, 409–421 (2017). https://doi.org/10.1007/s11240-016-1119-x

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  • DOI: https://doi.org/10.1007/s11240-016-1119-x

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