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Ectopic expression of ADP ribosylation factor 1 (SaARF1) from smooth cordgrass (Spartina alterniflora Loisel) confers drought and salt tolerance in transgenic rice and Arabidopsis

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Abstract

Salinity and drought are two very important abiotic stressors that negatively impact the growth and yield of all sensitive crop plants. Genes from halophytes have been shown to be useful to engineer crop plants that can survive under adverse soil and water conditions. The present report establishes, for the first time, the physiological role of a class one ADP ribosylation factor gene (SaARF1) from the halophyte Spartina alterniflora (smooth cordgrass) in imparting salinity and drought stress tolerance when expressed in both monocot (rice) and dicot (Arabidopsis) systems. The Arabidopsis and rice plants overexpressing ARF1 are many-fold more tolerant to salt and drought than wild-type (WT) plants. The transgenics exhibited improved growth and productivity relative to WT through tissue tolerance by maintaining higher relative water content and membrane stability, and higher photosynthetic yield by retaining higher chlorophyll concentration and fluorescence under stress conditions compared to WT. These findings indicated that genes from halophyte resources can be useful to engineer and improve salt and drought stress tolerance in both monocot and dicot plants.

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Acknowledgments

This research was supported by the Biotechnology AgCenter Interdisciplinary Team (BAIT) funding from the Louisiana State University Agricultural Center. The manuscript has been approved for publication by the Louisiana Agricultural Experimental Station as MS# 2013-306-11847.

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  1. Rohit Joshi

    Present address: International Center for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, 110067, India

Authors and Affiliations

  1. School of Plant, Environmental, and Soil Sciences, Louisiana State University Agricultural Center, Baton Rouge, LA, 70803, USA

    Rohit Joshi, Mangu Venkata Ramanarao, Seokhyun Lee & Niranjan Baisakh

  2. Department of Biological Sciences, Louisiana State University, Baton Rouge, LA, 70803, USA

    Naohiro Kato

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  1. Rohit Joshi
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Correspondence to Niranjan Baisakh.

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Joshi, R., Ramanarao, M.V., Lee, S. et al. Ectopic expression of ADP ribosylation factor 1 (SaARF1) from smooth cordgrass (Spartina alterniflora Loisel) confers drought and salt tolerance in transgenic rice and Arabidopsis . Plant Cell Tiss Organ Cult 117, 17–30 (2014). https://doi.org/10.1007/s11240-013-0416-x

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