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The promoter of soybean photoreceptor GmPLP1 gene enhances gene expression under plant growth regulator and light stresses

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Abstract

To reveal the molecular mechanism underlying the expression of soybean PLP1 (GmPLP1) gene that contains PAS/LOV domain, its 5′ regulatory region was analyzed. The 1.5 kbp fragment upstream of the GmPLP1 gene was isolated from soybean cultivar ‘Dongnong L13’ by genome walking. There were 107 cis-acting elements in the promoter predicted by online software Plant CARE and PLACE. The possible transcription start site (TSS) was confirmed by RACE (rapid-amplification of cDNA ends) and the promoter fragment deletions were inserted into the 5′ region of the gusA reporter gene in vector pBI121 (replacing the 35S promoter). In order to determine the promoter characteristic of GmPLP1, The constructs were transferred into Nicotiana tabacum and Glycine max. The GUS activity was up-regulated under dark, blue light, gibberellin A3 and abscisic acid driven by GmPLP1 promoter. The highest GUS enzyme activity was analyzed in transgenic plants at seedling and seed developmental stages. The high GUS enzyme level was detected in the leaves of tobacco and soybean seedlings, as well in seed formation when the full sequence of the promoter was present. The promoter activities decreased along with the plant being close to maturity. These results provided evidence of the developmental regulation of the GmPLP1 promoter in seedlings and developing seeds.

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Abbreviations

GmPLP1 :

Glycine max PAS/LOV protein 1

Phy:

Phytochrome

Cry:

Crytochrome

GUS:

β-Glucuronidase gene

LD:

Long days

SD:

Short days

SDP:

Short-day plant

LDP:

Long-day plant

DNP:

Day-neutral plant

ET:

Ethylene

ABA:

Abscisic acid

GA3:

Gibberellin A3

MeJA:

Methyl jasmonic acid

BA:

6-Benzyl aminopurine

IAA:

Indoleacetic acid

TSS:

Transcription start site

CTAB:

Cetyltriethylammnonium bromide

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Acknowledgments

This study was conducted in the Key Laboratory of Soybean Biology of Chinese Education Ministry and Soybean Development Centre of Agricultural Ministry, financially supported by National High Technology Project (2006AA100104-4), National Nature Science Foundation Projects (30971810, 60932008), Key Projects of Soybean Transformation (2011ZX08004-002, 2011ZX08004-005), National 973 Project (2009CB118400) and Provincial & Chinese Education Ministry for the team of soybean molecular design.

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Authors and Affiliations

  1. Key Laboratory of Soybean Biology in Chinese Ministry of Education, Northeast Agricultural University, Harbin, 150030, China

    Qiulan Luo, Yongguang Li, Hanqing Gu, Lin Zhao, Xianpeng Gu & Wenbin Li

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  1. Qiulan Luo
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  2. Yongguang Li
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  3. Hanqing Gu
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  4. Lin Zhao
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  6. Wenbin Li
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Correspondence to Wenbin Li.

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Qiulan Luo and Yongguang Li contributed equally to this work.

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Luo, Q., Li, Y., Gu, H. et al. The promoter of soybean photoreceptor GmPLP1 gene enhances gene expression under plant growth regulator and light stresses. Plant Cell Tiss Organ Cult 114, 109–119 (2013). https://doi.org/10.1007/s11240-013-0310-6

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