Abstract
Yam clones ‚Pacala Duclos’ and ‚Belep’ in temporary immersion system culture showed favourable results on shoot growth stage and in the development of microtubers in comparison with solid culture media. Cultures in temporary immersion systems in both clones obtained a higher microtuber number per plant, with greater fresh weight and diameter in comparison with solid culture media. Besides, 45 and 47% of microtubers greater than 3.0 gFW for ‚Belep’ and ‚Pacala Duclos’ clones respectively, were obtained. Those tubers may be planted without acclimatization and may be stored for a prolonged period of time.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
M Akita S Takayama (1994) ArticleTitleStimulation of potato (Solanum tuberosum L.) tuberization by semicontinuos liquid medium surface level control Plant Cell Rep 13 184–187
D Alvard F Cote C Teisson (1993) ArticleTitleComparison of methods of liquid medium culture for banana micropropagation. Effect of temporary immersion of explants Plant Cell Tiss. Org. Cult 32 55–60
M Basail V Medero A Santos M Cabrera J López J Ventura (2003) ArticleTitleEfecto de la densidad de explantes y el volumen de medio de cultivo en la micropropagación de la yuca en Sistema de Inmersión Temporal Revista de Biotecnología Vegetal, Vol. 3, No 3 IssueID2 93–96
R Bazabakana R Wattiez M Baucher B Diallo M Jaziri (2003) ArticleTitleEffect of jasmonic acid on developmental morphology during in vitro tuberization of Dioscorea alata (L.) Plant Growth Reg 40 229–23 Occurrence Handle10.1023/A:1025087111148
M Escalona JC Lorenzo B González M Daquinta JL González CG Desjardins Y & Borroto (1999) ArticleTitlePineapple (Ananas comosus LMerr) micropropagation in temporary immersion systems Plant Cell Rep 18 743–748 Occurrence Handle10.1007/s002990050653
H Etienne M Berthouly (2002) ArticleTitleTemporary immersion systems in plant micropropagation Plant Cell Tiss. Org. Cult 69 221–231 Occurrence Handle10.1023/A:1015668610465
E Jiménez N Pérez M DeFeria R Barbón A Capote M Chávez E Quiala J Pérez (1999) ArticleTitleImproved production of potato microtubers using a temporary immersion system Plant Cell Tiss. Org. Cult 59 19–23 Occurrence Handle10.1023/A:1006312029055
JC Lorenzo BL González M Escalona C Teisson P Espinosa C Borroto (1998) ArticleTitleSugarcane shoot formation in an improved temporary immersion system Plant Cell Tiss. Org. Cult 54 197–200 Occurrence Handle10.1023/A:1006168700556
S Mantell A Hugo (1989) ArticleTitleEffect of photoperiod mineral medium strength, inorganic ammonium, sucrose and cytokinina on root, shoot and microtuber development in shoot cultures of Dioscorea alata L. and D. Bulbifera L. yams Plant Cell Tiss. Org. Cult 16 23–39
P Martre D Lacan D Just C Teisson (2001) ArticleTitlePhysiological effects of temporary immersion on Hevea brasiliensis (Müll. Arg.) callus Plant Cell Tiss. Org. Cult 67 25–35 Occurrence Handle10.1023/A:1011666531233
T Murashige F Skoog (1962) ArticleTitleA revised medium for rapid growth and biossays with tobacco tissue culture Physiol. Plant 15 473–497
SYC Ng (1992) ArticleTitleMicropropagation of White yam (Dioscorea rotundata Poir.) Biotechnol. Agricult. Forest 19 137–159
R Salazar J Beltran (2002) ArticleTitleMicro tuberisación en ñame (Dioscorea alata L.) var ‚Pico de Botella’ Revista Colombiana de Biotecnología 2 27–32
C Teisson D Alvard B Berthouly F Cote JV Escalant M Etienne H & Lartaud (1996) ArticleTitleSimple apparatus to perform plant tissue culture by temporary immersion Acta Hort 440 521–526
Teisson C, Alvard D, Lartaud M, Etienne H, Berthouly M, Escalona M & Lorenzo JC (1999) Temporary immersion for plant tissue culture. In: Plant Biotechnology and in vitro Biology in the 21st Century, Proceedings of the Plant and Tissue and Cell Culture. Section H: Novel Micropropagation Methods (pp 629–632). Jerusalem
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Jova, M.C., Kosky, R.G., Pérez, M.B. et al. Production of yam microtubers using a temporary immersion system. Plant Cell Tiss Organ Cult 83, 103–107 (2005). https://doi.org/10.1007/s11240-005-4853-z
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s11240-005-4853-z