A new species of Neoergasilus Yin 1956 (Copepoda: Cyclopoida: Ergasilidae) parasitic on the catfish Clarias gariepinus (Burchell, 1822) (Siluriformes: Clariidae) from South Africa

Twenty-one specimens of an ergasilid were collected from the gills of the sharptooth catfish, Clarias gariepinus (Burchell), from the Eastern Cape, South Africa. The first leg and antennae morphology conformed to the genus Neoergasilus Yin, 1956. While the invasive Neoergasilus japonicus (Harada, 1930) is the only Neoergasilus species that has been reported from Africa, the combination of several characteristics, separates the Eastern Cape specimens from N. japonicus and the other eight Neoergasilus congeners. These include an inflated cephalothorax; the presence of an oval dorsal ornamentation anterior to the cephalosome; a spine on the posterodistal margin of the first antennal segment; a cone-like process at the proximal margin of the second antennal segment; a knob-like process on the inner distal margin of the first exopodal segment of leg 1 and two forked spines on the third exopodal segment; leg 4 bearing a 2-segmented exopod and 3-segmented endopod; a single-segmented fifth leg with a seta extending from the base of the pedigerous somite and three unequal setae on its free segment; and a median caudal rami seta with an array of spines. Supporting genetic data were generated using two partial ribosomal RNA genes, 18S and 28S, and one partial mitochondrial DNA gene, COI. The Eastern Cape species is here proposed as new to science and described as Neoergasilus africanus n. sp. (Ergasilidae: Cyclopoida). This is the first Neoergasilus species described from the sharptooth catfish and from the southern hemisphere. Additionally, a key to all the species of this genus is provided. Supplementary Information The online version contains supplementary material available at 10.1007/s11230-024-10189-6.


Introduction
Members of the family Ergasilidae Burmeister, 1835 occur globally as parasites of freshwater, brackish, and marine fishes.There are currently 30 accepted genera in the family (Hadfield, 2019;Walter & Boxshall, 2024a), of which three have been reported from African freshwater fishes, namely: Ergasilus von Nordmann, 1832; Neoergasilus Yin, 1956 (an invasive species); and Paraergasilus Markevich, 1937 (Oldewage & van As, 1988a, b;Berrouk et al., 2018Berrouk et al., , 2020;;Boucenna et al., 2018;Avenant-Oldewage et al., 2023;Fikiye et al., 2023).During parasitological surveys in South Africa, ergasilids were collected from the gills of the North African catfish, known locally as the sharptooth catfish, Clarias gariepinus (Burchell), in the Great Fish River, Eastern Cape, South Africa.This fish species has been translocated to the Great Fish River as a result of the Inter-Basin Water Transfer scheme from the Gariep Dam (Free State, South Africa) (see Cambray & Jubb, 1977).Currently, catfish populations are established in the Great Fish River and serve as hosts to several parasite species (see Truter et al., 2023a).The morphology of the ergasilid species collected from the current study conformed to the characteristics of members in the genus Neoergasilus.Similar to other ergasilid copepods, members of Neoergasilus are found attached to the gills and fins of their hosts (Hayden & Rogers, 1998;Hudson & Bowen, 2002;Alekseev et al., 2021).
Although the specimens from this study conform to the genus Neoergasilus, they differed morphologically from all other nine species in this genus.Limited genetic data are available for species of this genus.Currently, the only available sequences on GenBank are for N. japonicus for ribosomal RNA genes, 18S and 28S from the Czech Republic (Ondračková et al., 2019;Kvach et al., 2021), South Africa and Japan (Avenant- Oldewage et al., 2023); and the mitochondrial DNA gene, COI, from South Korea (Baek et al., 2016) and the United States of America (Vasquez et al., 2021 supplementary data).There is also a Gen-Bank submission from South Korea for 18S and 28S gene regions, but it is not associated with any peerreviewed article.This study, therefore, aims to characterise a parasitic copepod species morphologically and molecularly from the genus Neoergasilus not previously known to science as well as provide a key for taxon identification in this genus.

Sampling
Fifteen specimens of C. gariepinus were caught with baited longlines in November 2018 from the Great Fish River, Eastern Cape, South Africa (33°19′49.3″S26°59′54.2″E)(Fig. 1).Collected fish were examined for parasites attached to the body surface, fins, and gills, and were dissected and screened with the aid of a Zeiss Stemi 305 dissection microscope following standard methods for crustacean parasites as in Dávidová and Smit (2018).Adult female copepods (n = 21) were collected from the gills using fine paint brushes, needles, and tweezers, cleaned in Petri dishes containing freshwater, and fixed in 70% ethanol for morphological and molecular analyses.Host nomenclature is from FishBase (Froese & Pauly, 2024), host and parasite authorities (except for the synonymy) are not included in the references.

Morphological analysis
For morphology, photomicrographs of uncleared specimens were taken with the aid of a Zeiss Stemi 508 stereomicroscope (Zeiss, Oberkochen, Germany), then cleared in lactic acid for 60 minutes and stained in lignin pink for 10-20 minutes.With the aid of the stereomicroscope, six adult female specimens were dissected on glass microscope slides using fine needles, mounted temporarily with glycerine.Mounted specimens were viewed with bright-field and phasecontrast microscopy functions of a Nikon Eclipse 80i or Nikon Eclipse Ni differential interference contrast (DIC) microscopes (Nikon Instruments, Tokyo, Japan), enabling the z-dimensional stacking function.Photomicrographs of dissected appendages were taken using NIS-Elements BR Ver.4.60 software of the Nikon microscopes and drawn with the aid of a drawing tube microscope attachment.Measurements of 10 randomly selected specimens were made using the Labscope Mat Ver.2.8.4 microscope software connected to the Zeiss Stemi 508 stereomicroscope.All measurements are expressed in millimetres as mean ± standard deviation (with range in parentheses).Six ovigerous female specimens were selected for scanning electron microscopy (SEM).Specimens were dehydrated through a graded alcohol series; dried using hexamethyldisilazane (HMDS) or critically point dried; coated with gold-palladium and studied with the aid of a JEOL Winsem JSM IT 200 at 5 KVa.Illustrations were electronically inked using a Wacom Intuos® Pro drawing tablet and Adobe Illustrator™ software package.Morphological terminologies for the description followed Abdelhalim et al. (1993) and Boxshall (2016).

Infestation rates
Calculations for infestation rates were according to Bush et al. (1997), including prevalence, intensity, mean intensity, and mean abundance (± standard error).

Molecular analysis
Two protocols were used for genomic DNA extractions.The Macherey-Nagel tissue kit (GmbH & Co. KG, Sandton, South Africa) followed the manufacturer's protocol with an adaptation of the pre-lysis incubation period (3 hours ± 30 minutes in shaking incubator and a hold time of 2 hours), was used to extract genomic DNA from an ovigerous female specimen.The PCRBIO Rapid Extract PCR Kit (PCRBiosystems, Analytical Solutions, Randburg, South Africa) was also used for the extraction of genomic DNA using one egg string each from three separate specimens that had been selected for dissection, adapting the manufacturer's protocol as follows: 20 µl of lysis buffer, 10 µl of buffer containing protease, and 150 µl molecular grade water for dilution.
DNA amplifications for partial ribosomal RNA gene regions used primers designed by Song et al. (2008): 18SF and 18SR (18S), and 28SF and 28SR (28S); and the partial mitochondrial DNA gene region (COI) was amplified with primers by Bucklin et al. (2010): cop-Col-1498-F and cop-Col-2198-R (Table 1).Each Polymerase Chain Reaction (PCR) was carried out in 25 µl volumes using: 12.5 μl of DreamTaq PCR Master Mix (Thermo Fisher Scientific, South Africa); 1.25-2.5 μl of 10 μM of each primer (forward and reverse); and 2.5-10 μl of DNA product.The proportion of molecular grade water varied amongst the samples to make the final volume of 25 μl.DNA amplification was performed in a Benchmark TC9639 Thermal Cycler (Whitehead Scientific (Pty.) Ltd., Benchmark Scientific, USA) following conditions by Song et al. (2008) for 18S and adapting the conditions by Hayes et al. (2021) for 28S and COI (Table 1).Negative controls were used to detect possible contamination of the reagents.
Amplicons of PCR were verified by 1% agarose gel electrophoresis along with a 1kb DNA ladder (BioLabs Inc.).Positive PCR products were sent to a commercial sequencing company, Inqaba Biotechnical Industries (Pty) Ltd., Pretoria, South Africa, for purification and sequencing in both directions.Forward and reverse sequences received were checked for ambiguity, assembled, and edited using the bioinformatics software platform, Geneious Prime v. 2022.2.2 (Biomatters, Auckland, New Zealand; Kearse et al., 2012).Basic Local Alignment Search Due to the paucity of sequences from species in this genus, published and unpublished N. japonicus sequences were used in the alignment for pairwise genetic distance calculations.However, only sequences associated with peer-reviewed publications from the family Ergasilidae were used for the species of other ergasilid genera and Lernaea cyprinacea Linnaeus, 1758 (Lernaeidae) was used as the outgroup in this study (see Supplementary Table S1).Pairwise genetic distance matrices, expressed as the percentage similarity of bases and the number of base pair differences, were estimated in Geneious Prime v 2022.2.1.

Generic remarks
Individuals from the genus Neoergasilus are mainly characterised by the morphology of the first leg and antennae.The first leg is elongate, reaching fourth and fifth pedigerous somites in some specimens; the basis of the first leg has a triangular process at the posterior margin between the endopod and exopod; and an enlarged spatulate spine is present on the outer margin of the second exopodal segment of the first leg (Yin, 1956;Kim & Choi, 2003).In addition, the antennae of Neoergasilus are short, slender, and strongly curved, with a conspicuous spine (also referred to as a seta in Kim & Choi, 2003) present on the basal or second segment.The specimens from the current study conformed to the generic characteristics mentioned above.

Neoergasilus africanus n. sp.
Neoergasilus sp.Truter, Hadfield & Smit, 2023a: 170-179. Figs. 2, 3, 4 Material examined Holotype: Ovigerous female collected from the gills of Clarias gariepinus in the Great Fish River (33°19′49.3″S26°59′54.2″E),Eastern Cape, South Africa, collected by M. Truter (Nov. 2018).Deposited in the collections of the National Museum, Bloemfontein, South Africa (NMB P-1040).Paratypes: Five ovigerous females, same host and collection data as holotype.The specimens are deposited in the collections of the National Museum, Bloemfontein, South Africa (NMB P-1041).Other material: 13 ovigerous females, same host and collection data as holotype (six used for SEM; six dissected; one female and three egg strings used for DNA extraction).The remaining specimens are in the possession of the Water Research Group at the North-West University (NWU) Potchefstroom, South Africa.

ZooBank registration number:
Body cyclopiform (Figs.2a, 4a), comprising prosome consisting of cephalosome and first pedigerous somite fused (=cephalothorax), with anterodorsal oval ornamentation present and three free pedigerous somites (bearing legs 2 to 4), and urosome consisting of fifth pedigerous somite, genital double-somite and three free abdominal somites.Cephalothorax greatly expanded, 0.75 times as wide as long.Distinct lateral constriction defining cephalothorax and second pedigerous somite.Second pedigerous somite distinctly separated from cephalothorax; third and fourth somites gradually reducing in size; fifth somite reduced in size and grouped as part of urosome.Genital double somite with genital opening, three free abdominal somites, and caudal rami all ornamented with vertical row of minute spinules, observed ventrally (Figs.3b, 4d).Each abdominal somite overlapping, slightly larger than the next; anal somite with dorsoventral incision.Caudal ramus rectangular with four setae of varying lengths: median seta largest and longest, extending from the caudal ramus and ornamented with array of spines (Figs.3b, 4e).Egg sacs (Fig. 3c) 0.80 times length of body, multiseriate, occurring in pairs, with each sac containing approximately three columns of 8-10 eggs.
Leg 5 (Fig. 3g) represented by a long seta at dorsolateral margin of fifth pedigerous somite, a free segment bearing three setae of unequal lengths; one sub-terminal seta (the shortest) and two longer terminal setae.

Remarks
Neoergasilus africanus n. sp.differs from the nine known species of Neoergasilus by a combination of the following characteristics: an inflated cephalothorax 0.75 times as wide as it is long; the presence of an oval dorsal ornamentation anterior to the cephalosome; a knob-like process on the inner distal margin of the first exopodal segment of leg 1 and two forked spines on the third exopodal segment of leg 1; leg 4 bearing a 2-segmented exopod and 3-segmented endopod; a single-segmented fifth leg with three distal unequal setae and a seta on the lateral margin of the fifth pedigerous somite; an elongate spine on the Leg 1 0-0 0-1 I-0; I*-1; II-5 0-1; 0-1; II-4 Leg 2 0-0 0-1 I-0; 0-1; I-6 0-1; 0-2; I-4 Leg 3 0-0 0-1 I-0; 0-1; I-6 0-1; 0-2; I-4 Leg 4 0-0 0-1 I-0; I-5 0-1; 0-2; I-3 posterodistal margin of the first antennal segment, and cone-like basal process on proximal inner margin of its second antennal segment; the setation of the antennule; and the armature formula of the legs.When compared to the other described Neoergasilus species, the inflated cephalosome of N. africanus n. sp. is similar to N. squaliobarbi and N. bullatus; the remaining seven species have a more elongate cephalosome.Furthermore, the anterodorsal oval ornamentation on the cephalosome is absent in N. squaliobarbi and N. bullatus.The stout knob-like process on the inner distal margin of the first exopodal segment of leg 1 observed in N. africanus n. sp. is absent in N. squaliobarbi.Neoergasilus bullatus possesses a long blade-like inner distal process, much longer than the segment itself compared to the knoblike process observed in N. africanus n. sp.Additionally, the two spines on the third exopodal segment of leg 1 are forked in N. africanus n. sp.but not forked in both N. bullatus and N. squaliobarbi.The antenna of N. africanus n. sp. has a cone-like process at the base of its second segment, which is absent in N. bullatus and N. squaliobarbi.The exopod and endopod of the fourth leg of N. africanus n. sp. are 2-and 3-segmented respectively, sharing similarities with N. bullatus, but differing from N. squaliobarbi, which has a single-segmented exopod and 2-segmented endopod.The fifth leg of N. africanus n. sp. is single-segmented, with a seta on the margin of the fifth pedigerous somite, similar to N. bullatus.The free segment of leg 5 in N. africanus n. sp.bears three unequal setae; N. bullatus, however, has only a long plumose terminal seta and a minute lateral seta.In the case of N. squaliobarbi and its synonym N. inflatus, the authors report an absent fifth leg.
Geographically, only N. japonicus has been reported in the southern hemisphere (Mendes Marques & Murrieta Morey, 2019;Berrouk et al., 2018;2020;Avenant-Oldewage et al., 2023).The species described in this study differs from N. japonicus with several morphological characters.The cephalosome is inflated compared to the more elongate cephalosome of N. japonicus.Neoergasilus africanus n. sp. also has an anterodorsal oval ornamentation on the cephalothorax otherwise absent (or not observed) in the descriptions of N. japonicus.In addition to the elongate spine on the posterodistal margin of the first antennal segment, N. africanus n. sp. is ornamented with a basal conelike process at the proximal margin of its second antennal segment, which is absent on the antennae of N. japonicus.The fourth leg of N. africanus n. sp. has a 2-segmented exopod and a 3-segmented endopod, whereas N. japonicus possesses a singlesegmented endo-and exopod.Additionally, the spine-setae formulae of the legs are different for N. africanus n. sp. and N. japonicus.

Key to the species of Neoergasilus
To date, the only available key to the species of Neoergasilus was published by Kumari et al. (1988), comprising the five known species at the time, two of which were newly described by the authors.At that point, Smirnova (1971) had already synonymised N. inflatus with N. squaliobarbi (see Smirnova, 1971).Since then, four more species have been described and a fifth, N. africanus n. sp., is described here.The key below was constructed using drawings and texts of original descriptions and supporting descriptions of the 10 known species.The main morphological characteristics used in the key are illustrated in Fig. 5.

Molecular analysis
Seven novel sequences were generated from this study (three sequences for 18S, two sequences for 28S, and two sequences for COI).The phylogenetic alignments were done at family level since only one species from Neoergasilus, namely N. japonicus, has sequences available in GenBank (see Supplementary Table S1).
No phylogenetic alignments were done for the COI gene due to the limited number of sequences available, however, a preliminary alignment suggested that the primers used for DNA amplification of N. africanus n. sp.amplified a fragment of this gene gene that is different from the fragments amplified for the other Neoergasilus and Ergasilidae sequences available on GenBank.The COI sequences are therefore provided for future studies of this genus and family.

Discussion
The parasites collected from this study are placed in the genus Neoergasilus based on the morphology of the first swimming leg and features of the antennae.The genus Neoergasilus was originally described from Asia by Yin (1956) who described two new species, N. inflatus and N. longispinosus.Neoergasilus inflatus has since been synonymised with N. squaliobarbi (see Smirnova, 1971) because of the inflated cephalosome and teeth on the inner margin of the antennal claw (Fig. 5d).However, it appears that the synonymising of N. inflatus is not recognised by all authors or the status may still be unknown, as some authors still refer to N. inflatus as a recognised species (see Kim & Choi, 2003).The invasive species, N. japonicus is the only species from this genus that has been reported from Africa (Berrouk et al., 2018;2020;Boucenna et al., 2018;Avenant-Oldewage et al., 2023).The specimens from the current study, morphologically differ from N. japonicus as well as the other eight congeners by a combination of features described in this paper.
Within the genus Neoergasilus, certain morphological characters have been described differently by some authors.The antennae, for instance, which is 4-segmented (Abdelhalim et al., 1993;El-Rashidy & Boxshall, 2002;Boxshall, 2016), is referred to as the second antenna by earlier authors and reported as 5-segmented by others.The description of the Ergasilidae antennae as given by El-Rashidy and Boxshall (2002) is 4-segmented (coxobasis and three endopodal segments) with a claw (see Fig. 5c).The first antennal segment is the coxobasis, and the second to fourth antennal segments are the first to third endopodal segments (see antennal segments in Fig. 5c).It may be assumed that some authors have included the antennal claw as a segment (resulting in a 5-segmented antenna), rather than as an attachment to the third endopodal segment.Kumari et al. (1988), for instance, refer to the coxobasis as the first and second segments, and the terminal claw is referred to as the fifth segment.Also, Kim and Choi (2003), in their description of N. japonicus, omitted the fourth antennal segment, which is visible from their drawing and referred to the claw as the fourth segment.This might be because the fourth segment is greatly reduced and might easily be missed if not consciously inspected.
Furthermore, many of the earlier authors had either described the antennal spine to be on the second segment (Harada, 1930;Dogel' & Akhmerov, 1952;Vankara & Chikkam, 2010), at the margin between the second and third segment (Battish & Brar, 1989), or on the margin of the third segment (Kumari et al., 1988).It is also noteworthy that two characteristic structures of the antennae have been reported on the first and second (or second and third according to other earlier authors) antennal segments.The first is an elongate spine on the first segment (see red arrow in Fig. 5c and black arrow in Fig. 5e), which is usually at the distal margin of the segment and may also vary in length according to species; and the second is a slightly broader cone-like process, which is stout and pointed, almost extending from the base of the second segment (see red arrow in Fig. 5e).Four Neoergasilus species are described as having a slender spine on the first segment (N. angustus, N. bullatus, N. japonicus, N. longispinosus (see Yin, 1956;Kim & Choi, 2003)), while N. indicus and N. notopteri are described as having a cone-like process.Neoergasilus ferozepurensis is also described with a slender spine, and from the drawing, the spine is placed on the base of the second segment.For N. squaliobarbi (syn.N. inflatus) only a swollen disc-shaped process is reported on the second antennal segment (Dogel' & Akhmerov, 1952).The antennae of Neoergasilus species may also be armed with other non-characteristic setae, spines, and papillae along the inner and outer margins of different segments.Neoergasilus africanus n. sp., has two characteristic structures: the elongate spine on the first antennal segment, similar to N. angustus, N. bullatus, N. japonicus, and N. longispinosus; and a sharply pointed cone-like process extending from the basal end of the second antennal segment similar to N. notopteri and N. indicus.
Due to the limited sequences from this genus, an evolutionary relationship could not be estimated.Preliminary trees for 18S and 28S, however, support the position of this species as a member of the family Ergasilidae.

Conclusion
The specimens from this study are placed in the genus Neoergasilus based on the morphology of the first legs and antennae, they differ from the nine known species by a combination of morphological characteristics and have thus been described as new to science.These specimens were collected from the gills of the catfish C. gariepinus sampled from the Great Fish River in the Eastern Cape Province, South Africa.This is the first species from this genus to be described from Africa and the southern hemisphere and is the first Neoergasilus species to be described from this host.The only previous records of ergasilids from this catfish species include Ergasilus lamellifer Fryer, 1961, E. lizae Krøyer, 1863, E. mirabilis Oldewage & van As, 1987, and E. sarsi Capart, 1944(see Fikiye et al., 2023;Truter et al., 2023b).Apart from N. japonicus, there are no other Neoergasilus species with genetic data to explore the relationship of this new species in the genus.There is, therefore, a need for more genetic data to be provided with species description records in order to further understand the evolutionary relationship that exists within the genus Neoergasilus and the family Ergasilidae.
Funding Open access funding provided by North-West University.This research was funded by the National Research Foundation (NRF) (UID: 120403) and the KEFFES Rural Development Fund (KRDF).MT was funded by the NRF South African Research Chairs Initiative of the Department of Science and Innovation (DSI) (Inland Fisheries and Freshwater Ecology, Grant No. 11507).NJS and MT are in part supported by a Foundational Biodiversity Information Programme (FBIP) large grant from the National Research Foundation (NRF) of South Africa (Grant No. 138573).Opinions, findings, conclusions, and recommendations expressed in this publication are that of the authors, and the NRF accepts no liability whatsoever in this regard.The South African Institute for Aquatic Biodiversity (SAIAB) is acknowledged for infrastructure and equipment provided by the NRF-SAIAB Research Platforms and the funding channelled through the NFR-SAIAB Institutional Support system.

Data availability
All sequences generated from this study have been submitted in the GenBank database under the following Accession numbers PP864457-PP864459 (for 18S), PP864460-PP864461 (for 28S), and PP866728-PP866729 (for COI).The Holotype and paratypes from this study have been deposited in the collections of the National Museum, Bloemfontein, South Africa (NMB: P-1040 and NMB: P-1041).All other datasets generated during and/or analysed during the current study are available from the corresponding author upon reasonable request.

Conflict of interest
The authors declare no competing interests.
Ethical approval All procedures performed in studies involving animals were in accordance with the ethical standards of AnimCare at the North-West University.This study received necessary ethical clearance from The AnimCare Animal Research Ethics Committee of The North-West University (NWU-00159-18-A5; NWU-00600-19-A5).Research permits were obtained prior to sampling from the Department of Economic Development, Environmental Affairs and Tourism, Eastern Cape (permit no.CRO 20/18CR, CRO 22/18CR).
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made.The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material.If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

Fig. 4
Fig. 4 Scanning electron microscope (SEM) images of Neoergasilus africanus n. sp.adult female.(a) ventral view; with two egg strings; (b) ventral view of cephalosome showing spine and cone-like process of first and second antennal segments, respectively (red arrows), and maxilla (yellow arrow); (c) thoracic region showing denticles in segments of leg 1 (red circles) and knob-like process in inner margin of first exopodal segment of leg 1 (yellow arrow); (d) urosome; (e) median setae of caudal rami ornamented with an array of spines.Scale bars: (a) 100 µm; (b-d) 50 µm; (e) 5 µm.(Color figure online)

Fig. 5
Fig. 5 Illustrations of certain morphological characters used for keying out Neoergasilus Yin, 1956 species.(a) inflated cephalothorax with oval ornamentation (red arrow); (b) elongate cephalothorax without any ornamentation; (c) antenna showing the 1 st , 2 nd , 3 rd , 4 th segments and the claw; with scalpel-like spine (red arrow); (d) antenna highlighting short and broad claw with tooth-like projection on inner margin (red arrow); (e) antenna with short elongate spine (black arrow) and a cone-like basal process (red arrow); (f) leg 1 with knob-like bulging process (red arrow) on inner margin of first exopodal segment; (g) leg 1 with blade-like process (red arrow) on inner margin of first exopodal segment.Scale bars: (a, b) 100 µm; (c, g) 20 µm; (d-f) 50 µm.(Color figure online)

Table 1
List of primers with references, annealing temperatures, and the number of cycles used for DNA amplification of Neoer- Tool (BLAST) (https:// blast.ncbi.nlm.nih.gov/ Blast.cgi) was used to check the similarity between newly generated sequences with submissions of Neoergasilus available on GenBank as well as other copepods from the family Ergasilidae.

Table 2
Spine-setae formula for swimming legs of Neoergasilus africanus n. sp.from South Africa