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cDNA microarray analysis of early response to submerging stress in Zea mays roots

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Abstract

The complete expression profile of maize has not yet been published, especially at the early submerging treatment. These early-response genes play key roles in regulating the expressions of late-response genes leading to morphological and metabolic adaptations. In this study, a cDNA microarray platform has been developed to investigate the early submerging stress responses. Transcriptional level of many genes was markedly altered after 2-h long submerging treatment. The expressions of 32 cDNA clones, which represent 24 nonredundant genes, were more than threefold upregulated or downregulated under submerging stress as compared to their expression under nonsubmerged control conditions. Early-responsive to submergence genes are involved in signal transduction, glycolysis, photosynthesis, transcription and translation, and lipid and energy metabolic pathways. A full-length cDNA coined ZmZF (AY515607) was cloned from maize. Sequence analysis showed that ZmZF shares high homology with zinc finger-like proteins. In addition, expression of SKP1/ASK1-like protein and 20S proteasome subunit α-3 increased significantly after 2-h low-oxygen stress. Based on their expression pattern and function, we hypothesize that regulatory mechanisms at the translation level also play a crucial role in low-oxygen response at early submergence.

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Abbreviations

Adh1:

alcohol dehydrogenase 1

ANP:

anaerobic polypeptide

cat3:

catalase 3

eno:

enolase

gpd3:

glyceraldehyde-3-phosphate dehydrogenase

ZmZF:

Zea mays zinc finger-like protein

REFERENCES

  1. M.C. Drew C.J. He D.W. Morgen (2000) ArticleTitleProgrammed Cell Death and Aerenchyma Formation in Roots Trends Plant Sci. 5 123–127

    Google Scholar 

  2. A.M. Almeida W.H. Vriezen D. Straeten Particlevan der (2003) ArticleTitleMolecular and Physiological Mechanisms of Flooding Avoidance and Tolerance in Rice Russ. J. Plant Physiol. 50 743–751

    Google Scholar 

  3. M.M. Sachs M. Freeling R. Okimoto (1980) ArticleTitleThe Anaerobic Proteins of Maize Cell 20 761–767

    Google Scholar 

  4. D.A. Russell M.M. Sachs (1992) ArticleTitleProtein Synthesis in Maize during Anaerobic and Heat Stress Plant Physiol. 99 615–620

    Google Scholar 

  5. R.A. Kennedy M.E. Rumpho T.C. Fox (1992) ArticleTitleAnaerobic Metabolism in Plants Plant Physiol. 84 1204–1209

    Google Scholar 

  6. C.C. Subbaiah M.M. Sachs (2003) ArticleTitleMolecular and Cellular Adaptations of Maize to Flooding Stress Ann. Bot. (London) 90 119–127

    Google Scholar 

  7. S.K. Lal S. Johnson T. Conway P.M. Kelley (1991) ArticleTitleCharacterization of a Maize cDNA that Complements an Enolase-Deficient Mutant of Escherichia coli Plant Mol. Biol. 16 787–795

    Google Scholar 

  8. P.M. Kelley (1989) ArticleTitleMaize Pyruvate Decarboxylase mRNA Is Induced Anaerobically Plant Mol. Biol. 13 213–222

    Google Scholar 

  9. W.W. Chang L. Huang M. Shen C. Webster A.L. Burlingame J.K. Roberts (2000) ArticleTitlePatterns of Protein Synthesis and Tolerance to Anoxia in Root Tips of Maize Seedlings Acclimated to a Low-Oxygen Environment, and Identification of Proteins by Mass Spectrometry Plant Physiol. 122 295–318

    Google Scholar 

  10. I.N. Saab M.M. Sachs (1996) ArticleTitleA Flooding-Induced Xyloglucan Endotransglycosylase Homolog in Maize Is Responsive to Ethylene and Associated with Aerenchyma Plant Physiol. 112 385–391

    Google Scholar 

  11. B. Trevaskis R.A. Watts C.R. Andersson D.J. Llewellyn M.S. Hargrove J.S. Olson E.S. Dennis W.J. Peacock (1997) ArticleTitleTwo Hemoglobin Genes in Arabidopsis thaliana: The Evolutionary Origins of Leghemoglobins Proc. Natl. Acad. Sci. USA 94 12230–12234

    Google Scholar 

  12. E.J. Klok I.W. Wilson D. Wilson S.C. Chapman R.B. Ewing S.C. Somerville W.J. Peacock R. Dolferus E.S. Dennis (2002) ArticleTitleExpression Profile Analysis of the Low-Oxygen Response in Arabidopsis Root Cultures Plant Cell 14 2481–2494

    Google Scholar 

  13. E.S. Dennis W.L. Gerlach J.C. Walker M. Lavin W.J. Peacock (1988) ArticleTitleAnaerobic Regulated Aldolase Gene of Maize: A Chimaeric Origin? J. Mol. Biol. 202 759–767

    Google Scholar 

  14. M.R. Olive W.J. Peacock E.S. Dennis (1991) ArticleTitleThe Anaerobic Responsive Element Contains Two GC-Rich Sequences Essential for Binding a Nuclear Protein and Hypoxic Activation of the Maize Adh1 Promoter Nucleic Acids Res. 19 7053–7060

    Google Scholar 

  15. J.C. Walker E.A. Howard E.S. Dennis W.J. Peacock (1987) ArticleTitleDNA Sequences Required for Anaerobic Expression of the Maize Alcohol Dehydrogenase-1 Gene Proc. Natl. Acad. Sci. USA 84 6624–6628

    Google Scholar 

  16. E.S. Dennis R. Dolferus M. Ellis M. Rahman Y. Wu F.U. Hoeren A. Grover K.P. Ismond A.G. Good W.J. Peacock (2000) ArticleTitleMolecular Strategies for Improving Waterlogging Tolerance in Plants J. Exp. Bot. 51 89–97

    Google Scholar 

  17. J. Bailey-Serres M. Freeling (1990) ArticleTitleHypoxic Stress Induced Changes in Ribosomes of Maize Seedling Roots Plant Physiol. 94 1237–1243

    Google Scholar 

  18. C. Webster R.L. Gaut K.S. Browning J.M. Ravel J.K.M. Roberts (1991) ArticleTitleHypoxia Enhances Phosphorylation of Eukaryotic Elongation Factor 4A in Maize Root Tips J. Biol. Chem. 266 23341–23346

    Google Scholar 

  19. S. Manjunath A.J. Williams J. Bailey-Serres (1999) ArticleTitleOxygen Deprivation Stimulates Ca2+-Mediated Phosphorylation of mRNA Cap-Binding Protein eIF4E in Maize Roots Plant J. 19 21–30

    Google Scholar 

  20. S.L. Fennoy J. Bailey-Serres (1995) ArticleTitlePost-Transcriptional Regulation of Gene Expression in Oxygen-Deprived Roots of Maize Plant J. 7 287–295

    Google Scholar 

  21. S.L. Fennoy T. Nong J. Bailey-Serres (1998) ArticleTitleTranscriptional and Post-Transcriptional Processes Regulate Gene Expression in Oxygen-Deprived Roots of Maize Plant J. 15 727–736

    Google Scholar 

  22. C.H. Rhee K. Hess J. Jabbur M. Ruiz Y. Yang S. Chen A. Chenchik G.N. Fuller W. Zhang (1999) ArticleTitlecDNA Expression Array Reveals Heterogeneous Gene Expression Profiles in Three Glioblastoma Cell Lines Oncogene 18 2711–2717

    Google Scholar 

  23. L. Xu L. Hui S. Wang J. Gong Y. Jin Y. Wang Y. Ji X. Wu Z. Han G. Hu (2001) ArticleTitleExpression Profiling Suggested a Regulatory Role of Liver-Enriched Transcription Factors in Human Hepatocellular Carcinoma Cancer Res. 61 3176–3181

    Google Scholar 

  24. J. Sambrook D.W. Russell (2001) Molecular Cloning: A Laboratory Manual Cold Spring Harbor Laboratory Cold Spring Harbor A8.19

    Google Scholar 

  25. J.S. Scott-Craig J.E. Casida L. Poduje J.D. Walton (1998) ArticleTitleHerbicide Safener-Binding Protein of Maize: Purification, Cloning, and Expression of an Encoding cDNA Plant Physiol. 116 1083–1089

    Google Scholar 

  26. I. Lopez Ribera L. Ruiz-Avila P. Puigdomenech (1997) ArticleTitleThe Eukaryotic Translation Initiation Factor 5, eIF-5, a Protein from Zea mays, Containing a Zinc-Finger Structure, Binds Nucleic Acids in a Zinc-Dependent Manner Biochem. Biophys. Res. Commun. 236 510–516

    Google Scholar 

  27. I.P. Ribera P. Puigdomenech (1999) ArticleTitleStructure, Organization and Expression of the Eukaryotic Translation Initiation Factor 5, eIF-5, Gene in Zea mays Gene 240 355–359

    Google Scholar 

  28. A.J. Williams J. Werner-Fraczek I.F. Chang J. Bailey-Serres (2003) ArticleTitleRegulated Phosphorylation of 40S Ribosomal Protein S6 in Root Tips of Maize Plant Physiol. 132 2086–2097

    Google Scholar 

  29. D.A. Russell M.M. Sachs (1989) ArticleTitleDifferential Expression and Sequence Analysis of Maize Glyceraldhyde-3-Phospate Dehydrogenase Gene Family Plant Cell 1 793–803

    Google Scholar 

  30. N.C. Vetten ParticleDe R.J. Ferl (1995) ArticleTitleCharacterization of a Maize G-Box Binding Factor that Is Induced by Hypoxia Plant J. 7 589–601

    Google Scholar 

  31. C.C. Subbaiah M.M. Sachs (2001) ArticleTitleAltered Patterns of Sucrose Synthase Phosphorylation and Localization Precede Callose Induction and Root Tip Death in Anoxic Maize Seedlings Plant Physiol. 125 585–594

    Google Scholar 

  32. M.M. Sachs C.C. Subbaiah I.N. Saab (1996) ArticleTitleAnaerobic Gene Expression and Flooding Tolerance in Maize J. Exp. Bot. 47 1–15

    Google Scholar 

  33. S. Mackenzie L. Mclntosh (1999) ArticleTitleHigher Plant Mitochondria Plant Cell 11 571–585 Occurrence Handle10.1105/tpc.11.4.571 Occurrence Handle1:CAS:528:DyaK1MXjtFSrtbc%3D Occurrence Handle10213779

    Article  CAS  PubMed  Google Scholar 

  34. B.A. Schulman A.C. Carrano P.D. Jeffrey Z. Bowen E.R. Kinnucan M.S. Finnin S.J. Elledge J.W. Harper M. Pagano N.P. Pavletich (2000) ArticleTitleInsights into SCF Ubiquitin Ligases from the Structure of the Skp1-Skp2 Complex Nature 408 381–386

    Google Scholar 

  35. W.H. Shen Y. Parmentier H. Hellmann E. Lechner A. Dong J. Masson F. Granier L. Lepiniec M. Estelle P. Genschik (2002) ArticleTitleNull Mutation of AtCUL1 Causes Arrest in Early Embryogenesis in Arabidopsis Mol. Biol. Cell 13 1916–1928

    Google Scholar 

  36. N. Zheng B.A. Schulman L. Song J.J. Miller P.D. Jeffrey P. Wang C. Chu D.M. Koepp S.J. Elledge M. Pagano R.C. Conaway J.W. Conaway J.W. Harper N.P. Pavletich (2002) ArticleTitleStructure of the Cul1-Rbx1-Skp1-F Box Skp2 SCF Ubiquitin Ligase Complex Nature 416 703–709

    Google Scholar 

  37. O. Coux K. Tanaka A. L. Goldberg (1996) ArticleTitleStructure and Functions of the 20S and 26S Proteasomes Annu. Rev. Biochem. 65 801–847

    Google Scholar 

  38. M. Hochstrasser (1996) ArticleTitleUbiquitin-Dependent Protein Degradation Annu. Rev. Genet. 30 405–439

    Google Scholar 

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Authors and Affiliations

  1. National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, China

    Z. X. Zhang, W. H. Tang, Y. S. Tao & Y. L. Zheng

  2. College of Life Science, Yangtze University, 434025, Jingzhou, China

    Z. X. Zhang & W. H. Tang

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  1. Z. X. Zhang
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  2. W. H. Tang
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  3. Y. S. Tao
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  4. Y. L. Zheng
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From Fiziologiya Rastenii, Vol. 52, No. 1, 2005, pp. 51–58.

Original English Text Copyright © 2005 by Zhang, Tang, Tao, Zheng.

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Zhang, Z.X., Tang, W.H., Tao, Y.S. et al. cDNA microarray analysis of early response to submerging stress in Zea mays roots. Russ J Plant Physiol 52, 43–49 (2005). https://doi.org/10.1007/s11183-005-0007-8

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  • DOI: https://doi.org/10.1007/s11183-005-0007-8

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