Skip to main content
SpringerLink
Log in

Changes in Composition of Acid Soluble Proteins and DNA in Chromatin of Rat Liver and Brain Bound and Not Bound to Nuclear Envelope as a Function of Age and under the Influence of Antioxidant Ionol

  • Biochemistry of Development
  • Published:
Russian Journal of Developmental Biology Aims and scope Submit manuscript

Abstract

In two-day rat pups, the histone H1 content in the brain chromatin was higher than in the liver chromatin, as compared to histone of the nucleosome core. The H1 content in the brain chromatin decreased with the age, while in the liver chromatin it increased. At the same time, in the adult brain chromatin bound to the nuclear envelope, a high level of H1 characteristic of chromatin of the newborn rats was preserved, while in a similar chromatin of the adult liver, the H1 content increased, but still remained less than in the chromatin not bound to the nuclear envelope. In both organs, the composition and quantitation of H1 subfractions were different in chromatins bound and not bound to the nuclear envelope. The chromatin from the liver and brain bound to the nuclear envelope differed also in the composition and quantitation of minor acid soluble proteins. In the presence of the antioxidant ionol, the 5-methylcytosine content in DNA of chromatin of the rat liver bound to the nuclear envelope increased while in the chromatin not bound to the nuclear envelope, it remained unchanged. Thus the chromatins bound and not bound to the nuclear envelope differ in the composition and mount of acid soluble proteins, including histone H1, the contents of these proteins in bound and not bound chromatin are different and change with the age in different ways. The antioxidant ionol affects differently the methylation of bound and not bound chromatin.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

REFERENCES

  • Belmont, A., Dynamics of Chromatin, Proteins, and Bodies within the Cell Nucleus, Curr. Opin. Cell Biol., 2003, vol. 15, no.3, pp. 304–310.

    Article  CAS  PubMed  Google Scholar 

  • Bouvier, D., Hubert, J., Seve, A.P., and Bouteille, M., Characterization of Lamina-Bound Chromatin in the Nuclear Shell Isolated from HeLa Cells, Exp. Cell Res., 1985, vol. 156, no.2, pp. 500–512.

    Article  CAS  PubMed  Google Scholar 

  • Breneman, J.W., Yau, P., Teplitz, R.L., and Bradbury, E.M., A Light Microscope Study of Linker Histone Distribution in Rat Metaphase Chromosomes and Interphase Nuclei, Exp. Cell Res., 1993, vol. 206, no.1, pp. 16–26.

    Article  CAS  PubMed  Google Scholar 

  • Buschmann, M.B. and LaVelle, A., Morphological Changes of the Pyramidal Cell Nucleolus and Nucleus in Hamster Frontal Cortex during Development and Aging, Mech. Ageing Devel, 1981, vol. 15, no.4, pp. 385–397.

    CAS  Google Scholar 

  • Chanda, S.K., Ickowicz, R., and Dounce, A.L., High Total Histone Deoxyribonucleic Acid Ratios for Rat Liver Nuclei, Biochem. J., 1973, vol. 135, no.1, pp. 115–123.

    CAS  PubMed  Google Scholar 

  • Chaturvedi, M.M. and Kanungo, M.S., Analysis of Conformation and Function of the Chromatin of the Brain of Young and Old Rats, Mol. Biol. Rep., 1985, vol. 10, no.4, pp. 215–219.

    CAS  PubMed  Google Scholar 

  • Clark, P., Jones, K.J., and LaVelle, A.J., Ultrastructural and Morphometric Analysis of Nucleolar and Nuclear Changes During the Early Growth Period in Hamster Facial Neurons, Comp. Neurol., 1990, vol. 302, no.4, pp. 749–760.

    Article  CAS  Google Scholar 

  • Dominguez, V., Pina, B., and Suau, P., Histone H1 Subtype Synthesis in Neurons and Neuroblasts, Development, 1992, vol. 115, no.1, pp. 181–185.

    CAS  PubMed  Google Scholar 

  • Dubrovskii, I.V. and Berdyshev, G.D., Changes in Chromatin Nucleosome Compactization in the Cttle Liver during Aging, Ukr. Biokhim. Zh., 1986, vol. 58, no.3, pp. 8–13.

    CAS  PubMed  Google Scholar 

  • Fais, D., Prusov, A.N., and Polyakov, V.Yu., The Lack of Histone H1 in the Peripheral Chromatin of Rat Liver Nuclei, Cell Biol. Int. Rep., 1982, vol. 6, no.5, pp. 433–441.

    Article  CAS  PubMed  Google Scholar 

  • Greenwood, P.D. and Brown, I.R., Developmental Changes in DNAse I Digestibility and RNA Template Activity of Neuronal Nuclei Relative to the Postnatal Appearance of a Short DNA Repeat Length, Neurochem. Res., 1982, vol. 7, no.8, pp. 965–976.

    Article  CAS  PubMed  Google Scholar 

  • Greenwood, P.D., Silver, J.C., and Brown, I.R., Analysis of Histones Associated with Neuronal and Glial Nuclei Exhibiting Divergent DNA Repeat Lengths, J. Neurochem., 1981, vol. 37, no.2, pp. 498–505.

    CAS  PubMed  Google Scholar 

  • Hu, H., Xing, Y., Li, X., et al., DNase I Sensitivity of Nucleus and Chromatin in Six Different Tissues of Old Rats, Hua Xi Yi Ke Da Xue Xue Bao, 1999, vol. 30, no.4, pp. 405–407.

    CAS  PubMed  Google Scholar 

  • Huang, H.C. and Cole, R.D., The Distribution of H1 Histone Is Nonuniform in Chromatin and Correlates with Different Degrees of Condensation, J. Biol. Chem., 1984, vol. 259, no.22, pp. 14237–14242.

    CAS  PubMed  Google Scholar 

  • Kirnos, M.D., Vasilyev, V.K., and Vanyushin, B.F., Separation of Pyrimidine Deoxyribooligonucleotides According to Length and Composition Using Thin-Layer Chromatography on DEAE-Cellulose, J. Chromatogr., 1975, vol. 104, no.1, pp. 113–122.

    Article  CAS  PubMed  Google Scholar 

  • Konoplya, E.F., Detinkin, O.N., and Zhitkovich, A.V., Strength of Bonds in DNA-Protein Complexes of Rat Liver Nuclei during Aging, Vopr. Med. Khim., 1989, vol. 35, pp. 78–82.

    CAS  Google Scholar 

  • Kravchenko, L.S., Oksman, A.Ya., Glubokovskaya, I.O., and Tereshin, N.M., Effects of Polyene Antibiotics on DNP Fractions Bound and Not Bound to the Nuclear Envelope of Dog Kidney, Biokhimiya, 1979, vol. 41, no.1, pp. 133–142.

    Google Scholar 

  • Laemmli, U.K., Cleavage of Structural Proteins During the Assembly of the Head of Bacteriophage T4, Nature, 1970, vol. 227, pp. 680–685.

    Article  CAS  PubMed  Google Scholar 

  • Lennox, R.W. and Cohen, L.H., The Histone H1 Complements of Dividing and Nondividing Cells of the Mouse, J. Biol. Chem., 1983, vol. 258, no.1, pp. 262–268.

    CAS  PubMed  Google Scholar 

  • Lewis, C.D., Lebkowski, J.S., Daly, A.K., and Laemmli, U.K., Interphase Nuclear Matrix and Metaphase Scaffolding Structures, J. Cell Sci., 1984, vol. 1, no.1, pp. 103–122.

    CAS  Google Scholar 

  • Manteifel', V.M., Romanenko, E.B., Babadzhanyan, D.P., et al., An Electron Microscope Study of Chromatin in Nuclei of Rat Hepatocytes upon Age-Related Changes of Genome, Molecul. Biologiya (Moscow), 1988, vol. 22, no.4, pp. 1087–1096.

    Google Scholar 

  • Martou, G. and De Boni, U., Nuclear Topology of Murine, Cerebellar Purkinje Neurons: Changes as a Function of Development, Exp. Cell Res., 2000, vol. 256, no.1, pp. 131–139.

    Article  CAS  PubMed  Google Scholar 

  • Mitsui, Y., Sakagami, H., and Yamada, M., Histone H1 in G1 Arrested, Senescent, and Werner Syndrome Fibroblasts, Adv. Exp. Med. Biol., 1985, vol. 190, pp. 373–389.

    CAS  PubMed  Google Scholar 

  • Mizuno, N.S., Stoops, C.E., and Peiffer, R.L., Jr., Nature of the DNA Associated with the Nuclear Envelope of Regenerating Liver, J. Mol. Biol., 1971, vol. 59, pp. 517–525.

    Article  CAS  PubMed  Google Scholar 

  • Nielsen, J.A., Hudson, L.D., and Armstrong, R.C., Nuclear Organization in Differentiating Oligodendrocytes, J. Cell Sci., 2002, vol. 115, no.21, pp. 4071–4079.

    Article  CAS  PubMed  Google Scholar 

  • Park, P.C. and De Boni, U., Spatial Rearrangement and Enhanced Clustering of Kinetochores in Interphase Nuclei of Dorsal Root Ganglion Neurons in vitro: Association with Nucleolar Fusion, Exp. Cell Res., 1992, vol. 203, no.1, pp. 222–229.

    Article  CAS  PubMed  Google Scholar 

  • Pearson, E.C., Bates, D.L., Prospero, T.D., and Thomas, J.O., Neuronal Nuclei and Glial Nuclei from Mammalian Cerebral Cortex. Nucleosome Repeat Lengths, DNA Contents and H1 Contents, Eur. J. Biochem., 1984, vol. 144, no.2, pp. 353–360.

    Article  CAS  PubMed  Google Scholar 

  • Pina, B., Martinez, P., Simon, L., and Suau, P., Differential Kinetics of Histone H1(0) Accumulation in Neuronal and Glial Cells from Rat Cerebral Cortex during Postnatal Development, Biochem. Biophys. Res. Commun., 1984, vol. 123, no.2, pp. 697–702.

    Article  CAS  PubMed  Google Scholar 

  • Pina, B., Martinez, P., and Suau, P., Changes in H1 Complement in Differentiating Rat-Brain Cortical Neurons, Eur. J. Biochem., 1987, vol. 164, no.1, pp. 71–76.

    Article  CAS  PubMed  Google Scholar 

  • Prusov, A.N., Fais, D., and Polyakov, V.Yu., A Study of Peripheral Chromatin Granules—Anchorosomes, Biokhimiya, 1989, vol. 54, no.11, pp. 1838–1846.

    CAS  Google Scholar 

  • Prusov, A.N., Gazdarov, A.K., Koromyslov, G.F., and Vanyushin, B.F., Isolation and Composition of DNA of Chromatin Peripheral Layer Associated with Nuclear Envelope from Blood Lymphocytes of Healthy Cattle and Animals with Chronic Lympholeucosis, Biokhimiya, 1984, vol. 49, no.7, pp. 1203–1211.

    CAS  Google Scholar 

  • Puvion-Dutilleul, F., Azzarone, B., and Macieira-Coelho, A., Comparison between Proliferative Changes and Nuclear Events during Ageing of Human Fibroblasts in vitro, Mech. Ageing Devel., 1982, vol. 20, no.1, pp. 75–92.

    Article  CAS  Google Scholar 

  • Romanenko, E.B. and Vanyushin, B.F., Changes of DNA Methylation in Rats during development under the influence of Hydrocortisone, Biokhimiya, 1979, vol. 44, no.1, pp. 78–85.

    Google Scholar 

  • Romanenko, E.B., Alessenko, A.V., and Vanyushin, B.F., Effect of Sphingomyelin and Antioxidants on the in vitro and in vivo DNA Methylation, Biochem. Mol. Biol. Int., 1995, vol. 35, no.1, pp. 87–94.

    CAS  PubMed  Google Scholar 

  • Romanenko, E.B., Demidenko, Z.N., and Vanyushin, B.F., RNA-Polymerase, DNA-Polymerase, DNA-Methyltransferase, and Sphingomyelinase Activities in Liver Nuclei of Rats of Different Ages, Biokhimiya, 1998, vol. 63.

  • Romanenko, E.B., Pal'mina, N.P., and Vanyushin, B.F., Correlation between Increased DNA Methylation and Antioxidant Activity of Lipids of Mouse Liver Nuclei after Administration of Antioxidant and upon Ehrlich Carcinoma, Biokhimiya, 1979, vol. 44, no.10, pp. 1754–1761.

    CAS  Google Scholar 

  • Sjakste, N.I. and Budylin, A.V., Structural Changes of Chromatin at Different Levels of Its Organization during Aging, Ontogenez, 1992, vol. 23, no.3, pp. 242–253.

    CAS  PubMed  Google Scholar 

  • Schaffner, W. and Weismann, C., A Rapid Sensitive and Specific Method for the Determination of Protein in Dilute Solution, Anal. Biochem., 1973, vol. 56, pp. 502–514.

    Article  CAS  PubMed  Google Scholar 

  • Schmidt, G. and Thannhauser, S.J., A Method for the Determination of Deoxyribonucleic Acid, Ribonucleic Acid, and Phosphoproteins in Animal Tissues, J. Biol. Chem., 1945, vol. 161, pp. 83–87.

    CAS  Google Scholar 

  • Spirin, A.S., Spectrophotometric Assay of Total Amounts of Nucleic Acids, Biokhimiya, 1958, vol. 23, pp. 656–658.

    CAS  Google Scholar 

  • Tas, S. and Walford, R.L., Influence of Disulfide-Reducing Agents on Fractionation of the Chromatin Complex by Endogenous Nucleases and Deoxyribonuclease I in Aging Mice, J. Gerontol., 1982, vol. 37, no.6, pp. 673–679.

    CAS  PubMed  Google Scholar 

  • Vanyushin, B.F. and Berdyshev, G.D., Molekulyarno-geneticheskie mekhanizmy stareniya (Molecular-Genetic Mechanisms of Aging), Moscow: Nauka, 1977.

    Google Scholar 

  • Vanyushin, B.F., Lopatina, N.G., Wise, C.K., et al., Butylated Hydroxytoluene Modulates DNA Methylation in Rats, Eur. J. Biochem., 1998, vol. 256, no.3, pp. 518–527.

    Article  CAS  PubMed  Google Scholar 

  • Whatley, S.A., Hall, C., and Lim, L., Chromatin Organization in the Rat Hypothalamus during Early Development, Biochem. J., 1981, vol. 196, no.1, pp. 115–119.

    CAS  PubMed  Google Scholar 

  • Zongza, V. and Mathias, A.P., The Variation with Age of the Structure of Chromatin in Three Cell Types from Rat Liver, Biochem. J., 1979, vol. 179, no.2, pp. 291–298.

    CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Belozersky Research Institute of Physicochemical Biology, Moscow State University, Vorob'evy gory, Moscow, 119992, Russia

    A. N. Prusov, E. B. Romanenko & B. F. Vanyushin

Authors
  1. A. N. Prusov
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. E. B. Romanenko
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. B. F. Vanyushin
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

__________

Translated from Ontogenez, Vol. 36, No. 6, 2005, pp. 449–458.

Original Russian Text Copyright © 2005 by Prusov, Romanenko, Vanyushin.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Prusov, A.N., Romanenko, E.B. & Vanyushin, B.F. Changes in Composition of Acid Soluble Proteins and DNA in Chromatin of Rat Liver and Brain Bound and Not Bound to Nuclear Envelope as a Function of Age and under the Influence of Antioxidant Ionol. Russ J Dev Biol 36, 377–385 (2005). https://doi.org/10.1007/s11174-005-0054-2

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11174-005-0054-2

Key words

Search

Navigation