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Therapeutic Effect of Desmodium caudatum Extracts on Alleviating Diabetic Nephropathy Mice

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Abstract

Desmodium caudatum extracts (DCE) were investigated for their potential therapeutic effects on diabetic nephropathy (DN). In our study, the high-fat diet (HFD) / streptozotocin (STZ)-induced DN model in C57BL/6 mice was treated with 100 mg/kg, 200 mg/kg DCE. The results showed that DCE decreased biochemical parameters and proteinuria levels. The kidney sections staining indicated that DCE treatment recovered glomerular atrophy and alleviated lipid droplets in the glomerular. Additionally, DCE inhibited lipid and glycogen accumulation down-regulated the expression of sterol regulatory element-binding protein 1 (SREBP1) and fatty acid synthase (FAS) proteins. DCE also reduced collagenous fibrous tissue and the expression of transforming growth factor-β1 (TGF-β1) and alpha-smooth muscle actin (α-SMA) through Masson's trichrome staining and immunohistochemical analysis. We found that DCE alleviated hydroxyproline content, and epithelial-mesenchymal transition (EMT). Besides, the results shown that DCE enhanced the antioxidant enzymes to mitigate fibrosis by reducing oxidative stress. In conclusion, our study provided evidence of the protective effect of DCE which down-regulated hyperglycemia, hyperlipidemia and inhibition of TGF-β1 and EMT pathway but elevated antioxidant, suggesting its therapeutic implication for DN.

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Data Availability

The experimental data are included in the manuscript and are available from the corresponding author upon request.

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Funding

This study was supported by the National Science and Technology Council, Taiwan (NSTC110-2320-B040-015).

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Authors and Affiliations

  1. Department of Medical Laboratory and Biotechnology, Chung Shan Medical University, Taichung City, Taiwan

    Hui-Hsuan Lin

  2. Department of Nutrition, Chung Shan Medical University, No.110, Sec.1, Jiangou N. Rd., South Dist., Taichung City, 40201, Taiwan

    Chiao-Yun Tseng, Pei-Rong Yu, Hsiang-Yu Ho, Cheng-Chin Hsu & Jing-Hsien Chen

  3. Department of Medical Research, Chung Shan Medical University Hospital, Taichung City, Taiwan

    Jing-Hsien Chen

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  1. Hui-Hsuan Lin
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Contributions

J.-H.C. and H.-H.L. designed research; C.-Y.T. and H.-Y.H. performed experiments; C.-Y.T., P.-R.Y. and C.-C.H. analyzed data; C.-Y.T. and J.-H.C. wrote the manuscript; All authors reviewed and approved the manuscript.

Corresponding author

Correspondence to Jing-Hsien Chen.

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The animal experiments were approved by the Chung Shan Medical University Animal Care Committee (IACUC: approval number: 2426).

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Lin, HH., Tseng, CY., Yu, PR. et al. Therapeutic Effect of Desmodium caudatum Extracts on Alleviating Diabetic Nephropathy Mice. Plant Foods Hum Nutr (2024). https://doi.org/10.1007/s11130-024-01192-9

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