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The Effect of Light in Vitamin C Metabolism Regulation and Accumulation in Mung Bean (Vigna radiata) Germination

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Abstract

Vitamin C, as an essential vitamin for humans, has important physiological functions, such as antioxidants and enzyme cofactors. Mung bean sprouts are a good source of vitamin C and light is an essential impact factor of vitamin C content during germination. This study focused on the kinetic changes and metabolic regulation mechanism of vitamin C during mung bean sprouting under three-light treatment including constant light (24 h light/0 h dark), semi-light (12 h light/12 h dark) and constant dark (0 h light/24 h dark). Results confirmed that vitamin C content increased with the raised of light time during germination. The highest level of vitamin C, appeared on three days after constant light treatment (3-DALT), was 78 - fold higher than the initial concentration. L-ascorbic acid content on 3-DALT of mung bean sprouts was 21.4 and 29.8 times higher compared to the value on day 3 with semi -light treatment and constant dark treatment, respectively. Additionally, PMI, GME, GLDH, GalUR and DHAR expressions had strong correlations with L-ascorbic acid and vitamin C responding to light. Results indicated that light had an intimate correlation with the component and biosynthesis of vitamin C.

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Abbreviations

ASA:

Ascorbic acid

DADT:

Days after constant dark treatment

DALT:

Days after constant light treatment

DAST:

Days after semi-light treatment

DHA:

Dehydroascorbic acid

DHAR:

Dehydroascorbic acid reductase

GLDH:

L-galactonolactone dehydrogenase

GME:

GDP-mannose 3,5-epimerase

HAI:

Hours after imbibition

PMI:

Mannose-6-phosphate isomerase

RDA:

Recommended dietary allowance

ROS:

Reactive oxygen species

DT:

Dark treatment

LT:

Light treatment

ST:

Semi-light treatment

VC:

Vitamin C

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Acknowledgments

This research was funded by National Natural Science Foundation of China (31501765).

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  1. School of Food Science and Engineering, South China University of Technology, Guangzhou, 510640, China

    Yanyan Lu & Xinbo Guo

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  1. Yanyan Lu
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Correspondence to Xinbo Guo.

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Lu, Y., Guo, X. The Effect of Light in Vitamin C Metabolism Regulation and Accumulation in Mung Bean (Vigna radiata) Germination. Plant Foods Hum Nutr 75, 24–29 (2020). https://doi.org/10.1007/s11130-019-00787-x

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