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Proanthocyanidins Inhibit Iron Absorption from Soybean (Glycine max) Seed Ferritin in Rats with Iron Deficiency Anemia

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Abstract

The objective of this study was to evaluate the effect of proanthocyanidins (PAs) on iron uptake from soybean seed ferritin (SSF) crude by rats with iron deficiency anemia (IDA) for the first time. Six groups of Sprague–Dawley (SD) rats (n = 10) were used, which contain (1) SSF crude group; (2) SSF crude + PAs group; (3) PAs group; (4) FeSO4 group; (5) iron deficiency control group; and (6) control group. The bioavailability of iron was examined by measuring hemoglobin (Hb) concentration value, red blood cell (RBC) numbers, and serum iron stores. After 8 weeks, Hb concentration was almost recovered to the normal level upon feeding SSF crude or FeSO4 to rats. In contrast, Hb concentration was recovered to less extent when SSF crude plus PAs was used instead of SSF crude alone (P < 0.05). A similar profile was observed with these three sample groups when serum iron and RBC were used as parameters. All rats in PAs group died at the 8th week. Taken together, all these results demonstrated that PAs inhibited iron uptake of rats from SSF, and are toxic for rats with IDA.

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Abbreviations

PAs:

proanthocyanidins

SSF:

soybean seed ferritin

IDA:

iron deficiency anemia

SD:

Sprague–Dawley

SDS:

sodium dodecyl sulfate

Tris:

tris (hydroxymethyl) aminomethane

Hb:

hemoglobin

RBC:

red blood cell

DMT-1:

divalent metal transporter −1

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Acknowledgements

This project was supported by State Key Laboratory of Space Medicine Fundamentals and Application, China Astronaut Research and Training Center (SMFA09A012), the National Science and Technology Support Program (2011BAD23B04), and the Key Laboratory Open Fund of Soybean Biology of Education Ministry.

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Authors and Affiliations

  1. CAU & ACC Joint-Laboratory of Space Food, College of Food Science and Nutritional Engineering, China Agricultural University, Key Laboratory of Functional Dairy, Ministry of Education, Beijing Higher Institution Engineering Research Center of Animal Product, Beijing, 100083, People’s Republic of China

    Shaojun Yun, Tuo Zhang, Meiliang Li & Guanghua Zhao

  2. State Key Laboratory of Space Medicine Fundamentals and Application, China Astronaut Research and Training Center, Beijing, 100094, People’s Republic of China

    Bin Chen

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  1. Shaojun Yun
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  2. Tuo Zhang
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  3. Meiliang Li
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  4. Bin Chen
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  5. Guanghua Zhao
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Correspondence to Guanghua Zhao.

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Yun, S., Zhang, T., Li, M. et al. Proanthocyanidins Inhibit Iron Absorption from Soybean (Glycine max) Seed Ferritin in Rats with Iron Deficiency Anemia. Plant Foods Hum Nutr 66, 212–217 (2011). https://doi.org/10.1007/s11130-011-0240-6

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