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Changes induced by the Pepper mild mottle tobamovirus on the chloroplast proteome of Nicotiana benthamiana

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Abstract

We have analyzed the chloroplast proteome of Nicotiana benthamiana using two-dimensional gel electrophoresis and mass spectrometry followed by a database search. In order to improve the resolution of the two-dimensional electrophoresis gels, we have made separate maps for the low and the high pH range. At least 200 spots were detected. We identified 72 polypeptides, some being isoforms of different multiprotein families. In addition, changes in this chloroplast proteome induced by the infection with the Spanish strain of the Pepper mild mottle virus were investigated. Viral infection induced the down-regulation of several chloroplastidic proteins involved in both the photosynthetic electron-transport chain and the Benson–Calvin cycle.

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Abbreviations

2-DE:

Two-dimensional gel electrophoresis

CF1 :

ATP-ase coupling factor

CP:

Coat protein

Cyt. f:

Cytochrome f

FNR:

Ferredoxin-NADP+ reductase

GS:

Glutamine synthetase

Hsp70:

Heat-shock protein 70 kDa

IEF:

Isoelectrofocusing

Lhca:

Photosystem I chlorophyll a/b binding proteins

Lhcb:

Photosystem II chlorophyll a/b binding proteins

MALDI:

Matrix-assisted laser desorption/ionization

M r :

Relative molecular mass

MS:

Mass spectrometry

OEC:

Oxygen-evolving complex

PGK:

Phosphoglycerate kinase

pI:

Isoelectrical point

PMMoV-S:

Spanish strain of Pepper mild mottle virus

PRK:

Phosphoribulokinase

PsaC, PsaD and PsaE:

C, D and E proteins, respectively of the photosystem I core complex

PsbO, PsbP and PsbQ:

33, 24 and 16 kDa extrinsic proteins of the photosystem II oxygen-evolving complex

PSI:

Photosystem I

PSII:

Photosystem II

RbcL and RbcS:

Ribulose 1, 5 bisphosphate carboxylase oxygenase large and small subunits, respectively

RuBisCO:

Ribulose 1, 5 bisphosphate carboxylase oxygenase

Rca:

Ribulose 1, 5 bisphosphate carboxylase oxygenase activase

SBPase:

Sedoheptulose-1, 7-bisphosphatase

TOF/TOF:

Time-of-flight tandem mass spectrometry

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Acknowledgments

This research was supported by grants from the Spanish Government (BIO2004-04968-C02-02, BIO2007-67874-C02-02 and AGL2008-00214 to M.B.) and FEDER Funds. The authors are very grateful to Drs. Isabel García Luque and Maite Serra (Centro Investigaciones Biológicas, CSIC, Madrid) for providing all PMMoV solutions and antibodies against the viral-coat protein. In addition, they thank J.A. López, E. Camafeita and E. Calvo from the Proteomic Unit of the National Centre for Cardiovascular Research (Madrid, Spain) for the skilful assistance with the MALDI/TOF-TOF analysis and protein identification.

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  1. Department of Biochemistry, Molecular and Cellular Biology of Plants, Estación Experimental del Zaidín, (CSIC) C/Profesor Albareda no. 1, C.P. 18008, Granada, Spain

    M. Pineda, C. Sajnani & M. Barón

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  1. M. Pineda
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  2. C. Sajnani
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  3. M. Barón
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Correspondence to M. Barón.

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Pineda, M and Sajnani, C, These authors have contributed equally to this work and are placed in alphabetical order.

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Pineda, M., Sajnani, C. & Barón, M. Changes induced by the Pepper mild mottle tobamovirus on the chloroplast proteome of Nicotiana benthamiana . Photosynth Res 103, 31–45 (2010). https://doi.org/10.1007/s11120-009-9499-y

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  • DOI: https://doi.org/10.1007/s11120-009-9499-y

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