Abstract
Exposure of Photosystem II (PS II) membrane particles from spinach to a temperature of 47 °C caused the rapid release of the 18 kDa protein in parallel to inactivation of oxygen evolution. Previously, it has been suggested that the first heat-jump response involves rapid Ca release from the Mn complex of O2-evolution, followed by the slower release of (2 + 2) MnII ions [Pospisil P et al. (2003) Biophys J 84: 1370–1386]. Here, the predicted biphasic MnII release to the bulk was verified by atomic absorption spectroscopy (AAS). Analysis of laser flash-induced delayed fluorescence transients suggests that the loss of the essential Ca ion from the Mn4Ca complex in the dark is due to the loss of the 18 kDa protein. The S2-state multiline EPR signal of the Mn complex was still generated in heat-treated PS II presumably lacking Ca, but retaining four Mn ions.
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Abbreviations
- AAS:
-
atomic absorption spectroscopy
- APS:
-
ammonium persulfate
- Chl:
-
chlorophyll
- DF:
-
delayed fluorescence
- EPR:
-
electron paramagnetic resonance
- FWHM:
-
full width at half maximum
- LHC:
-
light harvesting complex
- MW:
-
molecular weight
- PAGE:
-
polyacrylamide gel electrophoresis
- PS II:
-
Photosystem II
- SDS:
-
sodiumdodecylsulfate
- TEMED:
-
tetramethylethyldiamine
- Tris:
-
tris-hydroxymethylethane sulfonic acid
- XAS:
-
X-ray absorption spectroscopy
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Dedicated to Professor Norio Murata on the occasion of his retirement
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Barra, M., Haumann, M. & Dau, H. Specific loss of the extrinsic 18 KDa protein from Photosystem II upon heating to 47 °C causes inactivation of oxygen evolution likely due to Ca release from the Mn-complex. Photosynth Res 84, 231–237 (2005). https://doi.org/10.1007/s11120-004-7158-x
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DOI: https://doi.org/10.1007/s11120-004-7158-x