Abstract
Plants are subjected to a variety of environmental stressors, including salinity, drought and pathogens, and have consequently developed a sophisticated sensing system. In Arabidopsis, malectin-like LRR-RLK impaired oomycete susceptibility 1 (IOS1, AT1G51800) plays an important role in both abiotic and biotic stresses by attenuating abscisic acid responses and regulating pattern-triggered immunity. This research aimed to investigate the role of IOS1 in regulating cellular responses to salinity. We compared the growth, antioxidant defence system, the expression of abiotic stress response marker genes and phosphorylation of MPKs between wild-type (Col-0) and ios1 mutant (SALK-137388) seedlings grown under salt stress. Our results demonstrated that the ios1 loss-of-function seedlings conferred more hypersensitivity to salinity. Salt stress-induced greater H2O2 accumulation in ios1 mutants than in Col-0 seedlings. Furthermore, salt stress-induced higher levels of ATL31, ZAT6, CRK13, CTP1, RD29A, RD29B, COR15A and RD22 in Col-0 than in ios1 plants after salt treatment. Moreover, ios1 mutants exhibited significantly weaker phosphorylation of MPK6 and accelerated dephosphorylation of activated MPK6 than Col-0 during NaCl treatment. These findings suggest that IOS1 is important and positively regulates salt stress in Arabidopsis.
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• Salt stress significantly induced IOS1 gene expression.
• The loss-of-function ios1 mutant showed lower salt tolerance than Col-0.
• The IOS1 protein was important for MPK6 activity.
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11105_2021_1301_MOESM1_ESM.tif
Supplementary file1 Figure S1. The phenotype of Col-0 and ios1on 1/2 MS plates with or without elicitors for 11 days. The vertical culture phenotype (A) and hypocotyl length (B) treated with Br or not for 5 days in dark. (n≥6, two-way ANOVA with Bonferroni test). C. The horizontal culture phenotype with or without metal for 15 days. (TIF 10814 KB)
11105_2021_1301_MOESM2_ESM.tif
Supplementary file2 Figure S2. The phenotype of bak1 mutant after stress. A. Schematic map of the BAK1 gene and position of the T-DNA insertion within the BAK1 in line SALK-116202. Boxes represent exons; lines represent untranslated regions. The transposon insertion was showed by triangle in the mutant, located to the nineth exon of the At4g33430 genomic region. RB right border, LB left border. The arrows indicate the primer target sites for analysis. B. The position of the T-DNA insertion in the mutant line were confirmed by genomic PCR. C. The horizontal culture phenotype of Col-0 and bak1on 1/2 MS plates with or without elicitors for 15 days. (TIF 3467 KB)
11105_2021_1301_MOESM7_ESM.docx
Supplementary file7 Supplemental file2. The promoter sequence of IOS1 used for cis-acting element analysis. (DOCX 15 KB)
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Cui, J., Hu, Y., Huang, Y. et al. Loss of Function Mutation of IOS1 in Arabidopsis Is More Sensitive to Salt Stress. Plant Mol Biol Rep 40, 68–80 (2022). https://doi.org/10.1007/s11105-021-01301-x
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DOI: https://doi.org/10.1007/s11105-021-01301-x