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Omics for understanding the mechanisms of Streptomyces lydicus A01 promoting the growth of tomato seedlings

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Abstract

Aims

Tomato is an important vegetable plant worldwide. Previously, we isolated a soilborne Actinobacteria species, Streptomyces lydicus A01, which promotes the growth of tomato seedlings. The related mechanisms are needed to study.

Methods

RNA sequencing and gas chromatography–mass spectrometry were used reveal the global effect of S. lydicus A01 on tomato seedlings. Liquid chromatography–mass spectrometry was used to detect plant hormones in tomato seedlings during the growth-promotion procedure.

Results

After administration of S. lydicus A01, tomato seedlings exhibit more vigorous growth with more leaflets and improved photosynthesis. It was revealed that S. lydicus A01 has some effects on the basic metabolism of tomato seedlings. Meanwhile, the biosynthesis of secondary metabolites related to plant growth was regulated, including cutin, suberine, wax and flavonoid. Additionally, plant hormones including abscisic acid, salicylic acid and jasmonic acid were discovered to involve in the plant-growth-promoting activity of S. lydicus A01.

Conclusions

This study revealed some growth-promoting mechanisms of tomato seedlings by S. lydicus A01, laying the groundwork for further research.

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Acknowledgements

This work was supported by Beijing Municipal Science and Technology Plan Projects (No. D151100003915002), Beijing Leafy Vegetables Innovation Team of Modern Agro-industry Technology Research System (BAIC07-2018), Science and Technology Innovation Ability Construction of BAAFS (No. KJCX20170415, No. KJCX20170107), Beijing Key Laboratory of Environment Friendly Management on Fruit Diseases and Pests in North China (No. BZ0432), National Key Research and Development Program of China (No. 2017YFD0200403, No. 2017YFD0201108), National Natural Science Foundation of China (No. 31270155), Science and Technology Innovation Team of Beijing Academy of Agriculture and Forestry Sciences (No. JNKST201607), and the National Special Project of Basic Work Project for Science and Technology (No. 2014FY120900). We sincerely thank Suzhou BioNovoGene Metabolomics Platform for the assistance in plant hormone detection with LC-MS and correlation analysis of GC-MS and RNA-seq data.

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Authors and Affiliations

  1. Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, China

    Qiong Wu, Mi Ni, Wei-Cheng Liu, Ying-Hong Rao & Cai-Ge Lu

  2. School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China

    Qiong Wu & Jie Chen

  3. Suzhou BioNovoGene Metabolomics Platform, Suzhou, 215000, China

    Jian-Hong Ren

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  1. Qiong Wu
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Responsible Editor: Hans Lambers.

Qiong Wu and Mi Ni are co-first authors

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Wu, Q., Ni, M., Liu, WC. et al. Omics for understanding the mechanisms of Streptomyces lydicus A01 promoting the growth of tomato seedlings. Plant Soil 431, 129–141 (2018). https://doi.org/10.1007/s11104-018-3750-2

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