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Comparison of cadmium-induced oxidative stress in Brassica juncea in soil and hydroponic cultures

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Abstract

Aims

The objective of this study was to investigate the response of Brassica juncea in the presence of Cd, in hydroponic and soil experiments, and to conclude about common and divergent trends in both cultures.

Methods

We studied the effect of Cd on growth, oxidative damage and antioxidant responses in roots and shoots of B. juncea grown in soil and hydroponic cultures, using typical time-scales for each one. Major ROS-scavenging enzymes such as catalase, ascorbate peroxidase and guaiacol peroxidase were evaluated, as well as lipid peroxidation.

Results

Small Cd concentrations in the plant led to enhanced plant growth, while large Cd concentrations impaired growth. The increase in lipid peroxidation observed in the presence of Cd was always greater in shoots than in roots. The physiological response to enhanced levels of reactive oxygen species in the presence of Cd included an increase in guaiacol peroxidase, ascorbate peroxidase and catalase activities, but those enzymes were not always enhanced in a similar manner in both cultures.

Conclusions

The main factors responsible for the differences between the experiments in soil and nutrient solution, were the different Cd content in the plant tissues and the different time-scale of the experiments.

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Acknowledgments

The authors acknowledge the Portuguese Foundation for Science and Technology for financial support under the project POCI-AMB/55312/2004 “Soil-plant transfer of trace elements. A phytoremediation study”.

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Authors and Affiliations

  1. CQE, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001, Lisboa, Portugal

    Teresa Armas, Maria Lurdes Simões Gonçalves & Ana Maria Mota

  2. ICAAM, Instituto de Ciências Agrárias e Ambientais Mediterrânicas, Universidade de Évora, Herdade Experimental da Mitra, 7000-676, Évora, Portugal

    Ana Paula Pinto

  3. CEER, Instituto Superior de Agronomia, Universidade de Lisboa, Tapada da Ajuda, 1349-017, Lisbon, Portugal

    Amarilis de Varennes

  4. UIQA, Instituto Superior de Agronomia, Universidade de Lisboa, Tapada da Ajuda, 1349-017, Lisbon, Portugal

    Miguel Pedro Mourato & Luísa Louro Martins

Authors
  1. Teresa Armas
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  2. Ana Paula Pinto
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  3. Amarilis de Varennes
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  4. Miguel Pedro Mourato
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  5. Luísa Louro Martins
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  6. Maria Lurdes Simões Gonçalves
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  7. Ana Maria Mota
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Corresponding author

Correspondence to Miguel Pedro Mourato.

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Responsible Editor: Juan Barcelo .

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Armas, T., Pinto, A.P., de Varennes, A. et al. Comparison of cadmium-induced oxidative stress in Brassica juncea in soil and hydroponic cultures. Plant Soil 388, 297–305 (2015). https://doi.org/10.1007/s11104-014-2330-3

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  • DOI: https://doi.org/10.1007/s11104-014-2330-3

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