Skip to main content
SpringerLink
Log in

Azospirilli of Uzbekistan Soils and their Influence on Growth and Development of Wheat Plants

  • Rhizosphere - Perspectives and Challenges - A Tribute to Lorenz Hiltner
  • Published:
Plant and Soil Aims and scope Submit manuscript

Abstract

Associative nitrogen-fixing bacteria have been isolated, which were related to Azospirillum genus, by their morphological–cultural and physiological–biochemical ability to grow in microaerophilic conditions, as well as by a number of phenotypic traits. They comprised two species, namely, Azospirillum brasilense and Azospirillum lipoferum. Azospirilli strains displayed a varying salt resistance on potato medium containing a range of NaCl concentrations from 100 to 800 mM. The decrease in the nitrogen-fixing activity of azospirilli was detected starting from 200 mM NaCl. The biomass of the inoculated local varieties of wheat, Unumdor Bugdoi and Karlik 85, in microvegetation experiments exceeded the biomass of control plants by 20–50%. During the vegetation, some azospirilli strains formed spontaneous nodules on the wheat roots.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • G Alexandre R Rohr R Bally (1999) ArticleTitleA phase variant of Azospirillum lipoferum lacks a polar flagellum and constitutively expresses mechanosensing lateral flagella Appl. Environ. Microbiol. 65 IssueID10 4701–4704 Occurrence Handle10508112 Occurrence Handle1:CAS:528:DyaK1MXms1OitLk%3D

    PubMed  CAS  Google Scholar 

  • T Bar Y Okon (1993) ArticleTitleTryptophan conversion to indole- 3-acetic acid via indole- 3-acetamide in Azospirillum brasilense Sp7 Can. J. Microbiol. 39 81–86 Occurrence Handle1:CAS:528:DyaK3sXhs1Cqtbo%3D

    CAS  Google Scholar 

  • P Barbieri E Galli (1993) ArticleTitleEffect on wheat root development of inoculation with an Azospirillum brasilense mutant with altered indole-3-acetic acid production Res. Microbiol. 144 69–75 Occurrence Handle8327784 Occurrence Handle1:CAS:528:DyaK3sXks1yhs7g%3D Occurrence Handle10.1016/0923-2508(93)90216-O

    Article  PubMed  CAS  Google Scholar 

  • C Christiansen-Weniger (1992) ArticleTitleN2 fixation by ammonium-excreting Azospirillum brasilense in auxin-induced root tumors of wheat (Triticum aestivum L.) Biol. Fertil. Soils 12 100–106 Occurrence Handle10.1007/BF00341483

    Article  Google Scholar 

  • A Costacurta V Keijers J Vanderleyden (1994) ArticleTitleMolecular cloning and sequence analysis of an Azospirillum brasilense indole-3-pyruvate decarboxylase gene Mol. Gen. Genet. 243 463–472 Occurrence Handle8202090 Occurrence Handle1:STN:280:DyaK2c3mtFWitg%3D%3D

    PubMed  CAS  Google Scholar 

  • S Dobbelaere A Croonenborghs A Thys A Vande Broek J Vanderleyden (1999) ArticleTitlePhytostimulatory effect of Azospirillum brasilense wild type and mutant strains altered in IAA production on wheat Plant Soil 212 155–164 Occurrence Handle1:CAS:528:DyaK1MXns1Gksrw%3D Occurrence Handle10.1023/A:1004658000815

    Article  CAS  Google Scholar 

  • J Döbereiner J M Day (1976 ) Associative symbioses in tropical grasses: characterization of microorganisms and dinitrogen-fxing sites W E Newton and C J Nyman (Eds) Proceedings of the 1st International Symposium on N2 Fixation Washington State University Press Pullman 518–538

    Google Scholar 

  • Gerhardt F, Murray R G E , Costilow R, Nester E W, Wood W A, Krieg N R and Philips G B, 1981 Manual of methods for general bacteriology. (1984 Translation from English to Russian under editing of Academician Kondratiyeva E N and Prof. Kalakuckii L V, Moscow, Publish. House Mir, 1, pp. 536)

  • S Fischer V Rivarola G Mori (2000) ArticleTitleColonization of wheat by Azospirillum brasilense Cd is impaired by saline stress Plant Soil 225 187–191 Occurrence Handle1:CAS:528:DC%2BD3MXotVGitQ%3D%3D Occurrence Handle10.1023/A:1026516313021

    Article  CAS  Google Scholar 

  • D W Hardy R Halstein E Jakson R S Buens (1968) ArticleTitle C2H2–C2H4 assay to N2 fixation laboratory and field evaluation Plant Phys. 43 9–13 Occurrence Handle10.1104/pp.43.1.9

    Article  Google Scholar 

  • A Hartmann (1988) ArticleTitlea Ecophysiological aspects of growth and nitrogen fixation in Azospirillum spp Plant Soil 110 225–238 Occurrence Handle1:CAS:528:DyaL1MXlsVWmuw%3D%3D Occurrence Handle10.1007/BF02226803

    Article  CAS  Google Scholar 

  • A Hartmann (1988 ) b Osmoregulatory properties of Azospirillum spp Klingmuller (Eds) Azospirillum IV Genetics-Physiology Ecology Springer-Verlag New York 122–130

    Google Scholar 

  • A Hartmann S R Prabhu E A Galinski (1991) ArticleTitleOsmotolerance of diazotrophic rhizosphere bacteria Plant Soil 137 105–109 Occurrence Handle1:CAS:528:DyaK3MXmslGntb4%3D Occurrence Handle10.1007/BF02187440

    Article  CAS  Google Scholar 

  • Holt J G, Krieg N R, Sheath P H A, Staley J T, Williams S T, 1994 Bergey`s Manual of Determinative Bacteriology. William and Wilkins. Baltimore. Philadelphia. Hong Kong. London. Munich. Sydney Tokyo. A Waverfly Company 1997 Translation from English to Russian under editing of Academician Zavarzin G A Moscow, Publish. House Mir, 1, pp 432

  • S Katupitiya J Millet M Vesk L Viccars A Zeman Z Lidong C Elmerich I Kennedy (1995) ArticleTitleA mutant of Azospirillum brasilense Sp7 impaired in flocculation with a modified colonization pattern and superior nitrogen fixation in association with wheat Appl. Environ. Microbiol. 61 1987–1995 Occurrence Handle7646034 Occurrence Handle1:CAS:528:DyaK2MXlsFyktbY%3D

    PubMed  CAS  Google Scholar 

  • M A Madkour L T Smith G M Smith (1990) ArticleTitlePreferential osmolyte accumulation: a mechanism of osmotic stress adaptation in diazotrophic bacteria Appl. Environ. Microbiol. 56 2876–2881 Occurrence Handle16348295

    PubMed  Google Scholar 

  • O N Shilyaeva Z M Yakovleva (1988) ArticleTitleDistribution of azospirilli in soils of Syberia Mikrobiologiya 57 2–284–287

    Google Scholar 

  • L Soto-Urzua Y G Xochinua-Corona M. Flores-Encarnacion B E Baca (1996) ArticleTitlePurification and properties of aromatic amino acid aminotransferases from Azospirillum brasilense UAP 14 strain Can. J. Microbiol. 42 294–298 Occurrence Handle8868238 Occurrence Handle1:CAS:528:DyaK28XhvVGjt74%3D Occurrence Handle10.1139/m96-043

    Article  PubMed  CAS  Google Scholar 

  • S Sriskandarajah I R Kennedy D Yu Y T Tchan (1993) ArticleTitleEffects of plant growth regulators on acetylene reducing associations between Azospirillum brasilense and wheat Plant Soil 153 165–177 Occurrence Handle1:CAS:528:DyaK2cXislGltQ%3D%3D Occurrence Handle10.1007/BF00012989

    Article  CAS  Google Scholar 

  • O Steenhoudt J Vanderleyden (2000) ArticleTitleAzospirillum, a free-living nitrogen-fixing bacterium closely associated with grasses: genetic, biochemical and ecological aspects FEMS Microbiology Reviews 24 487–506 Occurrence Handle10978548 Occurrence Handle1:CAS:528:DC%2BD3cXmt1Sjtrs%3D Occurrence Handle10.1111/j.1574-6976.2000.tb00552.x

    Article  PubMed  CAS  Google Scholar 

  • J J Tarrand N R Krieg J Döbereiner (1978) ArticleTitleA taxonomic study of the Spirillum lipoferum group, withdescriptions of a new genus, Azospirillum gen. nov. and two species, Azospirillum lipoferum (Beijerinck) comb. nov. and Azospirillum brasilense sp. nov Can. J. Microbiol. 24 967–980 Occurrence Handle356945 Occurrence Handle1:STN:280:DyaE1M%2FgtFersQ%3D%3D Occurrence Handle10.1139/m78-160

    Article  PubMed  CAS  Google Scholar 

  • L F Vasyuk A V Borovkov A E Khalchitcky S Ionkova Z V Vand Chmeleva (1989) ArticleTitleAzospirillum bacteria and their effect on the productivity of non-leguminous plants Mikrobiologiya 58 IssueID4 642–648

    Google Scholar 

  • A M M Zeman Y T Tchan C Elmerich I R Kennedy (1992) ArticleTitleNitrogenase activity in wheat seedlings bearing para-nodules induced by 2,4-dichlorophenoxyacetic acid (2,4-D) and inoculated with Azospirillum Res. Microbiol. 143 847–855 Occurrence Handle1299838 Occurrence Handle1:CAS:528:DyaK3sXitV2ntb4%3D Occurrence Handle10.1016/0923-2508(92)90072-V

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Microbiology of Uzbekistan Academy of Sciences, A. Kadyri street, Build. No. 7 B, 700128, Tashkent, Republic of Uzbekistan

    Shakirov Zair Saatovich

Authors
  1. Shakirov Zair Saatovich
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Shakirov Zair Saatovich.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Saatovich, S.Z. Azospirilli of Uzbekistan Soils and their Influence on Growth and Development of Wheat Plants. Plant Soil 283, 137–145 (2006). https://doi.org/10.1007/s11104-005-5690-x

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11104-005-5690-x

Keywords

Search

Navigation