Abstract
AtMSI4/FVE/ACG1, one of five Arabidopsis thaliana genes encoding MSI1-like proteins, helps determine plant growth and development (including control of flowering), as well as responses to certain biotic and abiotic stresses. We reasoned that the product of this gene, AtMSI4, acts through protein partners, which we have co-immunopurified with AtMSI4 from A. thaliana suspension culture cells and identified by liquid chromatography–mass spectrometry (LC–MS). Many of the proteins associated with AtMSI4 have distinct RNA recognition motif (RRM) domains, which we determined to be responsible for association with AtMSI4; and most of the associated RRM domain proteins also contain PWWP domains that are specific to plants. We propose these novel ATMSI4-associated proteins help form nucleoprotein complexes that determine pleiotropic functional properties of AtMSI4/FVE/ACG1 involving plant development and responses to stress.
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Abbreviations
- LC–MS:
-
Liquid chromatography–mass spectrometry
- GST:
-
Glutathione-S-transferase
- RRM:
-
RNA recognition motif
- SET:
-
Suppressor of variegation, enhancer of zeste and trithorax domain
- HDA:
-
Histone deacetylase
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Acknowledgments
We thank Dr. Tim Ulmasov for providing the cDNA library and CaMV35S transformation cassette vector, Dr. Barbara Ulmasov for help with transgenic plants, Ms. Linh Ngo for assistance with protein domain modeling, and Dr. Brian Mooney for assistance in LC–MS analyses. Financial support from the University of Missouri is gratefully acknowledged.
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Supplementary Fig. S1 Interaction of PWWP/RRM proteins with AtMSI5, AtMSI1 and RbAp48 (TIFF 7753 kb)
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Supplementary Fig. S2 Sequences at N-terminal regions of MSI1 like proteins. N-terminal region α-helix is involved in interaction with histone H4 (TIFF 13671 kb)
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Supplementary Fig. S3 Comparison of sequences from N-terminal region of At3G09670 involved in interaction with MSI4 with similar region of Arabidopsis homolog At5G02950 possessing RRM domain (Fig. S3a) and regions from homologs from relative species lacking RRM domain (Fig. S3b). (TIFF 34627 kb)
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Supplementary Table. S1 AtMSI4 associated proteins (* Proteins with only one peptide present in LC–MS pool. Interaction of RRM domain of At3G48190 with AtMSI4 was confirmed by GST-pull down assay) (DOCX 13 kb)
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Supplementary Table. S2 Combined list of AtMSI4 associated proteins from four independent immunoprecipitation identified by LC–MS (IPC-immunoprecipitation from cytoplasmic fraction; IPNE0.25 – 0.25 M NaCl nuclear extract; IPNE0.6 – 0.25-0.6 M nuclear extract; IPNL-nucleolar extract) (DOCX 16 kb)
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Kenzior, A., Folk, W.R. Arabidopsis thaliana MSI4/FVE associates with members of a novel family of plant specific PWWP/RRM domain proteins. Plant Mol Biol 87, 329–339 (2015). https://doi.org/10.1007/s11103-014-0280-z
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DOI: https://doi.org/10.1007/s11103-014-0280-z