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Gene expression analysis by cDNA-AFLP highlights a set of new signaling networks and translational control during seed dormancy breaking in Nicotiana plumbaginifolia

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Abstract

Seed dormancy in Nicotiana plumbaginifolia is characterized by an abscisic acid accumulation linked to a pronounced germination delay. Dormancy can be released by 1 year after-ripening treatment. Using a cDNA-amplified fragment length polymorphism (cDNA-AFLP) approach we compared the gene expression patterns of dormant and after-ripened seedS., air-dry or during one day imbibition and analyzed 15,000 cDNA fragments. Among theM., 1020 were found to be differentially regulated by dormancy. Of 412 sequenced cDNA fragmentS., 83 were assigned to a known function by search similarities to public databases. The functional categories of the identified dormancy maintenance and breaking responsive geneS., give evidence that after-ripening turns in the air-dry seed to a new developmental program that modulateS., at the RNA level, components of translational control, signaling networkS., transcriptional control and regulated proteolysis.

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Abbreviations

ABA:

Abscisic acid

cDNA-AFLP:

cDNA-amplified fragment length polymorphism

D:

Dormant

GA:

Gibberellin

ND:

Non-dormant

TDF:

Transcript derived fragment

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  1. Jérôme Bove

    Present address: Department of Biology, Pennsylvania State University, 208 Mueller Laboratory, University Park, PA, 16802-5301, USA

Authors and Affiliations

  1. UMR 204 de Biologie des Semences, Institut National de la Recherche Agronomique, Centre de Versailles-Grignon, route de Saint Cyr, 78026, Versailles Cedex, France

    Jérôme Bove, Philippe Lucas, Béatrice Godin, Laurent Ogé, Marc Jullien & Philippe Grappin

  2. Institut National Agronomique de Paris-Grignon, 16, rue Claude Bernard, 75231, Paris cedex 05, France

    Jérôme Bove, Philippe Lucas, Béatrice Godin, Laurent Ogé, Marc Jullien & Philippe Grappin

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  1. Jérôme Bove
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  2. Philippe Lucas
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Correspondence to Philippe Grappin.

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Bove, J., Lucas, P., Godin, B. et al. Gene expression analysis by cDNA-AFLP highlights a set of new signaling networks and translational control during seed dormancy breaking in Nicotiana plumbaginifolia. Plant Mol Biol 57, 593–612 (2005). https://doi.org/10.1007/s11103-005-0953-8

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