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Involvement of genes related to inflammation and cell cycle in Idiopathic Short Stature

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Abstract

Idiopathic Short Stature (ISS) defines a condition in which height is <−2SD compared to the mean of a reference population where systemic, endocrinological, nutritional or chromosomal disorders have not been identified and diagnosis is based on exclusion of any known causes of short stature. JAK/STAT pathway is triggered by GH binding to the GH receptor and promotes cellular growth through transcription of GH-responsive genes. In order to identify “candidate genes” differently expressed in ISS subjects with respect to control ones, we analyzed the expression of 84 genes related to JAK/STAT pathway by RT2 Profiler PCR array approach in a total of 10 subjects. Then, we validated the observed data by Real Time PCR and ELISA assays in a major number of subjects. We found two genes that were differently expressed in ISS subjects with respect to the control group: CXCL9 and FCGR1A/CD64, both significantly up-regulated (fold change 2.17 and 1.70, respectively) and belonging to family of IFN-γ-inducible factors. Further, ISS subjects showed an increased gene expression of IFN-γ and IFI16, higher serum levels of IFN-γ but similar levels of CXCL9 when compared to healthy subjects. In addition, we showed a pubertal modulation of CXCL9 levels. These data suggest that inflammatory and regulatory factors of the cell cycle may be involved in the ISS condition, introducing a new perspective to its etiology.

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Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Division of Pediatrics, Department of Medical Sciences, University of Piemonte Orientale, 28100, Novara, Italy

    Letizia Trovato, Flavia Prodam, Giulia Genoni, Francesca De Rienzo, Gillian E. Walker, Stefania Moia, Stefania Riccomagno, Simonetta Bellone & Gianni Bona

  2. Laboratory of Molecular and Cellular Endocrinology, Department of Internal Medicine, University of Turin, 10126, Turin, Italy

    Letizia Trovato

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  1. Letizia Trovato
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  2. Flavia Prodam
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  3. Giulia Genoni
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  4. Francesca De Rienzo
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  5. Gillian E. Walker
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  6. Stefania Moia
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  7. Stefania Riccomagno
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  8. Simonetta Bellone
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  9. Gianni Bona
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Correspondence to Letizia Trovato.

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Trovato, L., Prodam, F., Genoni, G. et al. Involvement of genes related to inflammation and cell cycle in Idiopathic Short Stature. Pituitary 16, 83–90 (2013). https://doi.org/10.1007/s11102-012-0378-8

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  • DOI: https://doi.org/10.1007/s11102-012-0378-8

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