Photosynthetica 2013, 51(4):621-629 | DOI: 10.1007/s11099-013-0060-z

Salinity response pattern and isolation of catalase gene from halophyte plant Aeluropus littoralis

M. Modarresi1,*, G. A. Nematzadeh2, F. Moradian3
1 Department of Plant Breeding, Faculty of Agriculture, Tarbiat Modares University, Tehran, I. R. Iran
2 Genetic and Agricultural Biotechnology Institute of Tabarestan, Sari Agricultural Sciences and Natural Resources University, Sari, I.R. Iran
3 Basic Sciences Group, Sari Agricultural Sciences and Natural Resources University, Sari, I.R. Iran

The role of the antioxidant defense system in salt tolerance of Aeluropus littoralis has not been yet reported; therefore in the present study, the changes of catalase (CAT) activity in this halophyte plant was investigated and CAT gene was isolated. The leaves of treated and control plants were harvested at various times, starting 1 day prior to initiating treatment, then periodically at 72-h intervals for 21 days. The data collected showed that CAT activity increased significantly with time in plants treated with 200, 400, and 600 mM NaCl when compared with the control plants. Maximum enzyme activity was observed between the 6th and 12th day at all NaCl concentrations. CAT gene was isolated and cloned via pTZ57R/T cloning vector in Escherichia coli. CAT gene encoded 494 amino acids and had also high homology of 90, 87, 86, and 86% with CAT genes from Zea mays, Oryza sativa, Triticum aestivum, and Hordeum vulgare, respectively.

Additional key words: bioinformatic analysis; catalase activity; gene isolation

Received: January 30, 2013; Accepted: April 8, 2013; Published: December 1, 2013  Show citation

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Modarresi, M., Nematzadeh, G.A., & Moradian, F. (2013). Salinity response pattern and isolation of catalase gene from halophyte plant Aeluropus littoralis. Photosynthetica, 51(4), 621-629. doi: 10.1007/s11099-013-0060-z
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