Photosynthetica 2011, 49(4):497-506 | DOI: 10.1007/s11099-011-0062-7

Photoprotective function of betacyanin in leaves of Amaranthus cruentus L. under water stress

T. Nakashima1,*, T. Araki2, O. Ueno3
1 Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Fukuoka, Japan
2 Faculty of Agriculture, Ehime University, Ehime, Japan
3 Faculty of Agriculture, Kyushu University, Fukuoka, Japan

The photoprotective function of leaf betacyanin in water-stressed Amaranthus cruentus plants was examined by comparing leaves of two strains which differ significantly in the amount of betacyanin. At 0, 1, and 2 days after the imposed water stress, leaves were subjected to high-light (HL) treatment to assess their photosynthetic capacity and photoinhibition susceptibility. The water stress equally reduced leaf relative water content (RWC), gas-exchange rate and chlorophyll (Chl) contents in both leaves, indicating that the severity of water stress was comparable between the strains. Consequently, the extent of photoinhibition after the HL treatment increased in both strains as water stress developed; however, it was significantly greater in acyanic leaves than in betacyanic leaves, suggesting lower photoinhibition susceptibility in the betacyanic strain. The betacyanic leaves also exhibited approximately 30% higher values for photochemical quenching coefficient (qP) during the period of water stress despite the nonphotochemical quenching coefficient (qN) did not differ significantly between the strains. These results may be partially explained by the increased amount of leaf betacyanin under water stress. Moreover, a decrease in Chl content in betacyanic leaves might have enhanced light screening effect of betacyanin by increasing relative abundance of betacyanin to Chl molecule. In addition, reduced Chl content increased light penetrability of leaves. As a result, the extent of photoinhibition at the deeper tissue was exacerbated and the Chl fluorescence emitted from these tissues was more readily detected, facilitating assessment of photoinhibition at deeper tissues where the effect of betacyanic light screening is considered to be most apparent. Our results demonstrated that leaf betacyanin contributes to total photoprotective capacity of A. cruentus leaves by lowering excitation pressure on photosystem II (PSII) via attenuation of potentially harmful excess incident light under water stress.

Additional key words: betacyanin; grain amaranthus; light screening; maximum quantum yield of photosystem II; photoinhibition; water deficit

Received: January 12, 2011; Accepted: July 15, 2011; Published: December 1, 2011  Show citation

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Nakashima, T., Araki, T., & Ueno, O. (2011). Photoprotective function of betacyanin in leaves of Amaranthus cruentus L. under water stress. Photosynthetica, 49(4), 497-506. doi: 10.1007/s11099-011-0062-7
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