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Non-Propellant Foams of Green Nano-Silver and Sulfadiazine: Development and In Vivo Evaluation for Burn Wounds

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Abstract

Purpose

A non-propellant based foam (NPF) system was developed incorporating the antibiotics, pectin capped green nano-silver and sulfadiazine (SD) for the topical treatment of burn wounds as a convenient alternative to the existing therapies.

Methods

NPF were prepared using various surfactants and oils forming a nanoemulsion. Anti-microbial studies by resazurin microtitre assay, ex vivo diffusion, in vivo skin permeation and deposition studies, and acute irritation studies were carried out. NPF was applied onto secondary thermal wounds manifested on mice models followed by macroscopic and histological examinations.

Results

NPF had an average globule size of <75 nm. The viscosity was ~10 cP indicating the feasibility of expulsion from the container upon actuation. With no skin irritation, the foams showed a higher skin deposition of SD. A high contraction and an evident regeneration of the skin tissue upon treatment with NPF indicated a good recovery from the thermal injury was apparent from the histology studies.

Conclusion

NPF represents an alternative topical formulation that can be employed as a safe and effective treatment modality for superficial second degree (partial thickness) burn wounds. With a minimal requirement of mechanical force, the no-touch application of NPF makes it suitable for sensitive and irritant skin surfaces.

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Abbreviations

AgF:

Silver foam

AgSD:

Silver sulfadiazine

BLF:

Blank foam

nAg:

Nano-silver

nAgF:

Nano-silver foam

nAgSD:

Nano-silver sulfadiazine

NPF:

Non-propellant foams

PF:

Propellant foams

REMA:

Resazurin microtitre assay

RFD:

Relative foam density

SD:

Sulfadiazine

SDF:

Sulfadiazine foam

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Acknowledgements

The authors are grateful to Gattefossé India Pvt. Ltd, Mumbai for providing generous samples of Labrasol®, Plurol Oleique CC 497® and Labrafac Lipophile WL 1349® for carrying out the studies.

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Authors and Affiliations

  1. Department of Pharmaceutics, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education, Manipal, Karnataka, 576104, India

    Aleksandra Kurowska, Vivek Ghate & Shaila A. Lewis

  2. Department of Post-Graduate Studies and Research in Chemistry, Mangalore University, Mangalagangothri, (DK), Karnataka, 574199, India

    Arun Kodoth & Badalamoole Vishalakshi

  3. Department of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education, Manipal, Karnataka, 576104, India

    Aarti Shah

  4. Department of Pharmacognosy, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education, Manipal, Karnataka, 576104, India

    Abhishek Shah

  5. Department of Microbiology, University College, Hampanakatte, Mangalore, Karnataka, 575001, India

    Bharathi Prakash

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  1. Aleksandra Kurowska
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Kurowska, A., Ghate, V., Kodoth, A. et al. Non-Propellant Foams of Green Nano-Silver and Sulfadiazine: Development and In Vivo Evaluation for Burn Wounds. Pharm Res 36, 122 (2019). https://doi.org/10.1007/s11095-019-2658-8

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