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Enhancing Effect of Glucose Microspheres in the Viability of Human Mesenchymal Stem Cell Suspensions for Clinical Administration

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Abstract

Purpose

A critical limiting factor of cell therapy is the short life of the stem cells. In this study, glucose containing alginate microspheres were developed and characterized to provide a sustained release system prolonging the viability of human mesenchymal stem cells (hMSCs) in a suspension for clinical application.

Methods

The glucose microspheres were satisfactorily elaborated with alginate by emulsification/internal gelation method. Particle size was evaluated by light diffraction and optical microscopy. Shape and surface texture by scanning electron microscopy (SEM). Zeta potential, infrared spectra and release studies were also conducted. Also, rheological properties and stability of hMSCs suspensions with microspheres were tested. The viability of hMSCs was determined by trypan blue dye exclusion staining.

Results

Microspheres of 86.62 μm, spherical shaped and −32.54 mV zeta potential with excellent stability, good encapsulation efficiency and providing an exponential release of glucose were obtained. hMSCs had better survival rate when they were packed with glucose microspheres. Microspheres maintained the aseptic conditions of the cell suspension without rheological, morphological or immunophenotypic disturbances on hMSCs.

Conclusions

Developed microspheres were able to enhance the functionality of hMSC suspension. This strategy could be broadly applied to various therapeutic approaches in which prolonged viability of cells is necessary.

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Abbreviations

AIC::

Akaike’s Information Criterion

EE::

Encapsulation Efficiency

FITC::

Fluorescein Isothiocyanate

FTIR::

Fourier Transforms Infrared Spectroscopy

hMSCs::

Human Mesenchymal Stem Cells

LC::

Loading Capacity

PBS::

Phosphate Buffered Saline

PE::

Phycoerythrin

PY::

Percentage Yield

SEM::

Scanning Electron Microscopy

TPB::

Thioglycollate Penase Broth

TSPB::

Tryptic Soy Penase Broth

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Acknowledgments

Financial support from project MAT2011-26994 (MCNN-Ministerio de Ciencia e Innovacion) is acknowledged. This research work has been also partially funded by the CEI BioTIC Granada. We thank CABIMER’s GMP Staff for cell preparation and characterization. Thanks are also extended to Dr Lyda Halbaut Bellowa from Barcelona University (Spain) for excellent technical expertise.

Author information

Authors and Affiliations

  1. Andalusian Centre for Molecular Biology and Regenerative Medicine (CABIMER), Seville, Spain

    Patricia Gálvez

  2. Department of Pharmacy and Pharmaceutical Technology, School of Pharmacy, University of Granada, Campus of Cartuja St., 18071, Granada, Spain

    Maria J. Martín, Maria A. Ruiz & Beatriz Clares

  3. Biopharmaceutical and Pharmacokinetics Unit, School of Pharmacy, University of Barcelona, Barcelona, Spain

    Ana C. Calpena

  4. Department of Medicinal and Organic Chemistry, School of Pharmacy, University of Granada, Granada, Spain

    Juan A. Tamayo

Authors
  1. Patricia Gálvez
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  2. Maria J. Martín
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  3. Ana C. Calpena
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  4. Juan A. Tamayo
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  5. Maria A. Ruiz
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  6. Beatriz Clares
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Corresponding author

Correspondence to Beatriz Clares.

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Gálvez, P., Martín, M.J., Calpena, A.C. et al. Enhancing Effect of Glucose Microspheres in the Viability of Human Mesenchymal Stem Cell Suspensions for Clinical Administration. Pharm Res 31, 3515–3528 (2014). https://doi.org/10.1007/s11095-014-1438-8

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  • DOI: https://doi.org/10.1007/s11095-014-1438-8

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