The chemical composition of bupleurum plant is complex. The main active components of bupleurum are saponins, volatile oils, flavonoids and polysaccharides, exerting multiple pharmacological effects. Here, we have first found that saikosaponin-d (SSD) reduced kidney injury and inflammation, induced SIRT1, and suppressed protein expression on in vivo mice model of acute kidney injury (AKI). In LPS-induced kidney cell inflammation model, SSD suppressed IL-1B, NLRP3, SIRT1 and ROS expression as detected after cells were treated with SSD. Furthermore, the anti-inflammation effects of SSD on inflammation and kidney injury were detected on in vivo mice model of ARI via NLRP3 expression. The results showed that ROS inhibitor increased anti-inflammation effects of SSD on in vivo mice inflammation model and in vitro model. Finally, NLRP3 inhibitor also reduced anti-inflammation effects of SSD on in vivo mice inflammation model and in vitro model. On the whole, these findings confirm that SSD prevents AKI via inhibition of NLRP3 inflammasome by SIRT1.
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All animal procedures in this study were approved by the Institutional Animal Care and Use Committee of the Hebei General Hospital
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Lin Kang was dedicated to the integrity of the entire study; Fan Yang carried out the study concepts, definition of intellectual content, data acquisition and manuscript editing; Xiuzhi Zhang was involved in the manuscript preparation; Jing Zhao focused on the experimental studies; Yang Liu carried out the statistical analysis; Huanfen Zhao was involved in the manuscript review; Zhijuan Hu focused on the clinical studies and data analysis; Bing Liu was dedicated to the study design; Chunnian He carried out the literature research. All authors have read and approved this article.
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Kang, L., Yang, F., Zhang, X. et al. Saikosaponin-D Prevents Acute Renal Injury via Inhibition of NLRP3 Inflammasome By SIRT1. Pharm Chem J 55, 1169–1176 (2022). https://doi.org/10.1007/s11094-022-02554-w
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DOI: https://doi.org/10.1007/s11094-022-02554-w