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A Liquid Chromatography Method for Simultaneous Determination of Diphenhydramine, Promethazine, Chlorpheniramine and Ephedrine in Cold-Cough Syrups

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Pharmaceutical Chemistry Journal Aims and scope

Asimple, rapid isocratic liquid chromatography method was developed for the simultaneous determination of diphenhydramine, promethazine, chlorpheniramine, and ephedrine in cold-cough syrups commonly available in the Kenyan market. The influence of the percentage of organic modifier, ion pairing agent, buffer concentration as well as pH and column temperature on the selectivity with respect to analytes was investigated. Optimum chromatographic separation was achieved using a C18 Gemini® NX column (250 mm × 4.6 mm, 5 μm) maintained at 40°C and a mobile phase comprising methanol – triethylamine – 0.2 M ammonium acetate pH 5.0 – water mixture (50 : 0.15 : 40 : 9.85, v/v) delivered at a flow rate of 1.0 mL/min. Upon validation, the proposed liquid chromatography method satisfied the International Committee on Harmonization acceptance criteria for linearity, sensitivity, precision, and robustness. The method was applied in the analysis of commercial samples obtained from Nairobi County, Kenya. The method can be used in routine analysis of cold-cough syrups containing the specified compounds.

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Acknowledgements

The work presented in this paper was a part of Master of Pharmacy thesis of NMN, University of Nairobi (2009). The authors wish to thank the Director and Board of Management of the National Quality Control Laboratory for financial support and providing the necessary instruments/apparatus to perform this study.

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Authors and Affiliations

  1. Department of Pharmaceutical Chemistry, School of Pharmacy, University of Nairobi, P. O. Box 19676–00202, Nairobi, Kenya

    Nicholas M. Njuguna, Kennedy O. Abuga, Franco N. Kamau & Grace N. Thoithi

  2. National Quality Control Laboratory, P. O. Box 29726-00202, Nairobi, Kenya

    Nicholas M. Njuguna

Authors
  1. Nicholas M. Njuguna
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  2. Kennedy O. Abuga
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  3. Franco N. Kamau
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  4. Grace N. Thoithi
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Correspondence to Kennedy O. Abuga.

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Njuguna, N.M., Abuga, K.O., Kamau, F.N. et al. A Liquid Chromatography Method for Simultaneous Determination of Diphenhydramine, Promethazine, Chlorpheniramine and Ephedrine in Cold-Cough Syrups. Pharm Chem J 51, 153–158 (2017). https://doi.org/10.1007/s11094-017-1574-7

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  • DOI: https://doi.org/10.1007/s11094-017-1574-7

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