Abstract
This work is dedicated to the measurement of plasma proteins concentration in liquid solutions using Raman spectroscopy setup. The obtained Raman spectra showed significant variation of intensities of certain spectral bands 940, 1005, 1330, 1450 and 1650 cm−1 for different protein fractions and concentrations. Partial least squares regression analysis was used for determination of correlation coefficients. It has been shown that the proposed method represents structure and biochemical composition of albumin and immunoglobulins A and G, and that albumin concentration may be measured with an accuracy of 6–8 g/L.
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Anderson, N.L., Anderson, N.G.: The human plasma proteome: history, character, and diagnostic prospects. Mol. Cell. Proteomics MCP 1(11), 845–867 (2002)
Berger, A.J., Koo, T.W., Itzkan, I., Horowitz, G., Feld, M.S.: Multi-component blood analysis by near-infrared Raman spectroscopy. Appl. Opt. 38(13), 2916–2926 (1999)
Dingari, N.C., Horowitz, G.L., Kang, J.W., Dasari, R.R., Barman, I.: Raman spectroscopy provides a powerful diagnostic tool for accurate determination of albumin glycation. PLoS ONE 7(2), e32406 (2012). doi:10.1371/journal.pone.0032406
Enejder, A.M., Koo, T.W., Oh, J., Hunter, M., Sasic, S., Feld, M.S., Horowitz, G.L.: Blood analysis by Raman spectroscopy. Opt. Lett. 27(22), 2004–2006 (2002)
Farrugia, A.: Albumin usage in clinical medicine: tradition or therapeutic? Transfus. Med. Rev. 24(1), 53–63 (2010)
Gonzàlez-Quintela, A., Alende, M.R., Gamallo, R., Gonzàlez-Gil, P., López-Ben, S., Tomé, S., Otero, E., Torre, J.A.: Serum immunoglobulins (IgG, IgA, IgM) in chronic hepatitis C. A comparison with non-cirrhotic alcoholic liver disease. Hepatogastroenterology 50(54), 2121–2126 (2003)
Kengne-Momo, R.P., Daniel, Ph, Lagarde, F., Jeyachandran, Y.L., Pilard, J.F., Durand-Thouand, M.J., Thouand, G.: Protein interactions investigated by the Raman spectroscopy for biosensor applications. Int. J. Spectrosc. 2012, 462901 (2012)
Rao, S., Bálint, S., Cossins, B., Guallar, V., Petrov, D.: Raman study of mechanically induced oxygenation state transition of red blood cells using optical tweezers. Biophys. J. 96(1), 209–216 (2009)
Rhoades, R.A., Pflanzer, R.G.: Human Physiology, 4th edn. Thomson Learning, Stockholm (2002)
Sjöström, M., Wold, S., Lindberg, W., Persson, J.-Å., Martens, H.: A multivariate calibration problem in analytical chemistry solved by partial least-squares models in latent variables. Anal. Chim. Acta 150, 61–70 (1983)
Wang, H., Lee, A.M., Lui, H., McLean, D.I., Zeng, H.: A method for accurate in vivo micro-Raman spectroscopic measurements under guidance of advanced microscopy imaging. Sci. Rep. 3, 1890 (2013). doi:10.1038/srep01890
Wang, J., Lin, D., Lin, J., Yu, Y., Huang, Z., Chen, Y., Lin, J., Feng, S., Li, B., Liu, N., Chen, R.: Label-free detection of serum proteins using surface-enhanced Raman spectroscopy for colorectal cancer screening. J. Biomed. Opt. 19(8), 087003 (2014). doi:10.1117/1.JBO.19.8.087003
Wold, S., Sjostrom, M., Eriksson, L.: PLS-regression: a basic tool of chemometrics. Chemometr. Intell. Lab. Syst. 58(2), 109–130 (2001)
Zakharov, V.P., Bratchenko, I.A., Myakinin, O.O., Artemyev, D.N., Kornilin, D.V., Kozlov, S.V., Moryatov, A.A.: Multimodal diagnosis and visualisation of oncologic pathologies. Quantum Electron. 44(8), 726–731 (2014)
Zakharov, V.P., Bratchenko, I.A., Artemyev, D.N., Myakinin, O.O., Kornilin, D.V., Kozlov, S.V., Moryatov, A.A.: Comparative analysis of combined spectral and optical tomography methods for detection of skin and lung cancers. J. Biomed. Opt. 20(2), 025003 (2015). doi:10.1117/1.JBO.20.2.025003.
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This research was supported by the Ministry of Education and Science of the Russian Federation.
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This article is part of the Topical Collection on Laser technologies and laser applications.
Guest Edited by José Figueiredo, José Rodrigues, Nikolai A. Sobolev, Paulo André and Rui Guerra.
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Artemyev, D.N., Zakharov, V.P., Davydkin, I.L. et al. Measurement of human serum albumin concentration using Raman spectroscopy setup. Opt Quant Electron 48, 337 (2016). https://doi.org/10.1007/s11082-016-0610-2
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DOI: https://doi.org/10.1007/s11082-016-0610-2