Skip to main content
Log in

Measurement of human serum albumin concentration using Raman spectroscopy setup

  • Published:
Optical and Quantum Electronics Aims and scope Submit manuscript

Abstract

This work is dedicated to the measurement of plasma proteins concentration in liquid solutions using Raman spectroscopy setup. The obtained Raman spectra showed significant variation of intensities of certain spectral bands 940, 1005, 1330, 1450 and 1650 cm−1 for different protein fractions and concentrations. Partial least squares regression analysis was used for determination of correlation coefficients. It has been shown that the proposed method represents structure and biochemical composition of albumin and immunoglobulins A and G, and that albumin concentration may be measured with an accuracy of 6–8 g/L.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Anderson, N.L., Anderson, N.G.: The human plasma proteome: history, character, and diagnostic prospects. Mol. Cell. Proteomics MCP 1(11), 845–867 (2002)

    Article  Google Scholar 

  • Berger, A.J., Koo, T.W., Itzkan, I., Horowitz, G., Feld, M.S.: Multi-component blood analysis by near-infrared Raman spectroscopy. Appl. Opt. 38(13), 2916–2926 (1999)

    Article  ADS  Google Scholar 

  • Dingari, N.C., Horowitz, G.L., Kang, J.W., Dasari, R.R., Barman, I.: Raman spectroscopy provides a powerful diagnostic tool for accurate determination of albumin glycation. PLoS ONE 7(2), e32406 (2012). doi:10.1371/journal.pone.0032406

    Article  ADS  Google Scholar 

  • Enejder, A.M., Koo, T.W., Oh, J., Hunter, M., Sasic, S., Feld, M.S., Horowitz, G.L.: Blood analysis by Raman spectroscopy. Opt. Lett. 27(22), 2004–2006 (2002)

    Article  ADS  Google Scholar 

  • Farrugia, A.: Albumin usage in clinical medicine: tradition or therapeutic? Transfus. Med. Rev. 24(1), 53–63 (2010)

    Article  Google Scholar 

  • Gonzàlez-Quintela, A., Alende, M.R., Gamallo, R., Gonzàlez-Gil, P., López-Ben, S., Tomé, S., Otero, E., Torre, J.A.: Serum immunoglobulins (IgG, IgA, IgM) in chronic hepatitis C. A comparison with non-cirrhotic alcoholic liver disease. Hepatogastroenterology 50(54), 2121–2126 (2003)

    Google Scholar 

  • Kengne-Momo, R.P., Daniel, Ph, Lagarde, F., Jeyachandran, Y.L., Pilard, J.F., Durand-Thouand, M.J., Thouand, G.: Protein interactions investigated by the Raman spectroscopy for biosensor applications. Int. J. Spectrosc. 2012, 462901 (2012)

    Article  Google Scholar 

  • Rao, S., Bálint, S., Cossins, B., Guallar, V., Petrov, D.: Raman study of mechanically induced oxygenation state transition of red blood cells using optical tweezers. Biophys. J. 96(1), 209–216 (2009)

    Article  Google Scholar 

  • Rhoades, R.A., Pflanzer, R.G.: Human Physiology, 4th edn. Thomson Learning, Stockholm (2002)

  • Sjöström, M., Wold, S., Lindberg, W., Persson, J.-Å., Martens, H.: A multivariate calibration problem in analytical chemistry solved by partial least-squares models in latent variables. Anal. Chim. Acta 150, 61–70 (1983)

    Article  Google Scholar 

  • Wang, H., Lee, A.M., Lui, H., McLean, D.I., Zeng, H.: A method for accurate in vivo micro-Raman spectroscopic measurements under guidance of advanced microscopy imaging. Sci. Rep. 3, 1890 (2013). doi:10.1038/srep01890

    ADS  Google Scholar 

  • Wang, J., Lin, D., Lin, J., Yu, Y., Huang, Z., Chen, Y., Lin, J., Feng, S., Li, B., Liu, N., Chen, R.: Label-free detection of serum proteins using surface-enhanced Raman spectroscopy for colorectal cancer screening. J. Biomed. Opt. 19(8), 087003 (2014). doi:10.1117/1.JBO.19.8.087003

    Article  ADS  Google Scholar 

  • Wold, S., Sjostrom, M., Eriksson, L.: PLS-regression: a basic tool of chemometrics. Chemometr. Intell. Lab. Syst. 58(2), 109–130 (2001)

    Article  Google Scholar 

  • Zakharov, V.P., Bratchenko, I.A., Myakinin, O.O., Artemyev, D.N., Kornilin, D.V., Kozlov, S.V., Moryatov, A.A.: Multimodal diagnosis and visualisation of oncologic pathologies. Quantum Electron. 44(8), 726–731 (2014)

    Article  ADS  Google Scholar 

  • Zakharov, V.P., Bratchenko, I.A., Artemyev, D.N., Myakinin, O.O., Kornilin, D.V., Kozlov, S.V., Moryatov, A.A.: Comparative analysis of combined spectral and optical tomography methods for detection of skin and lung cancers. J. Biomed. Opt. 20(2), 025003 (2015). doi:10.1117/1.JBO.20.2.025003.

    Article  ADS  Google Scholar 

Download references

Acknowledgments

This research was supported by the Ministry of Education and Science of the Russian Federation.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Dmitry N. Artemyev.

Additional information

This article is part of the Topical Collection on Laser technologies and laser applications.

Guest Edited by José Figueiredo, José Rodrigues, Nikolai A. Sobolev, Paulo André and Rui Guerra.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Artemyev, D.N., Zakharov, V.P., Davydkin, I.L. et al. Measurement of human serum albumin concentration using Raman spectroscopy setup. Opt Quant Electron 48, 337 (2016). https://doi.org/10.1007/s11082-016-0610-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s11082-016-0610-2

Keywords

Navigation