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Nutritional Support of Crocin on Neurobehavioral Disabilities Induced by Cisplatin-Based Chemotherapy in Rats

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Abstract

Cisplatin, a chemotherapy drug containing platinum, is considered a neurotoxic agent. On the other hand, crocin, the primary component of saffron, possesses neuroprotective and antioxidant properties. In this study, 28 healthy adult male Wistar rats weighing 200–250 g were used (6–7 weeks old). Rats were divided into a control group (Ctr), a crocin group (Cro), a cisplatin group (Cis), and a crocin with cisplatin group (Cro + Cis). Rotarod, open field, and shuttle box tests were performed to assess balance, explorative behavior, and avoidance memory. After behavioral testing, the hippocampus was extracted to analyze oxidative stress parameters such as GPx (glutathione peroxidase), SOD (superoxide dismutase), CAT (catalase), and MDA (malondialdehyde) activity. Shuttle box, rotarod, and open field results showed that crocin can substantially mitigate the deleterious effects of cisplatin on avoidance memory, explorative behavior, motor coordination, and balance. Crocin was also able to effectively avoid the negative effects of cisplatin on MDA, GPx, and CAT during the assessment of oxidative indicators, while the beneficial effect of crocin on cisplatin was not statistically significant in terms of SOD level. In conclusion, since free radicals produced by cisplatin are a contributing factor to memory loss and movement disorders, crocin, owing to its antioxidant properties, improved passive avoidance learning as well as motor activity.

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Data Availability

The datasets generated for this study are available on request to the corresponding author.

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Acknowledgements

We are grateful to the Research Council of Shahid Chamran University of Ahvaz for financial supports and Dr. Mina Jahangiri for the detailed and accurate statistical analysis of the data.

Funding

We are grateful to the Research Council of Shahid Chamran University of Ahvaz for financial support.

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Authors and Affiliations

  1. Department of Basic Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

    Zohreh Ghotbeddin

  2. Stem Cell and Transgenic Technology Research Center, Shahid Chamran University of Ahvaz, Ahvaz, Iran

    Zohreh Ghotbeddin

  3. Department of Veterinary Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

    Mohsen Peysokhan, Anahita Memar Dezfouli & Mohammad Asadi Iraee

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  1. Zohreh Ghotbeddin
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  2. Mohsen Peysokhan
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Contributions

ZG participated in study design, data collection, evaluation and contributed to behavioral experiments and was responsible for overall supervision. MP, AMD and MAI contributed to all experimental work and data statistical analysis. All the authors reviewed and approved the final manuscript.

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Correspondence to Zohreh Ghotbeddin.

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Ethical Approval

Animal studies were conducted according to the protocols and guidelines approved by the Institutional Ethics Committee of Shahid Chamran University of Ahvaz (EE/97.24.3.49914/scu.ac.ir) and were conducted under the Guide for the Care and Use of Laboratory Animals (NIH).

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Ghotbeddin, Z., Peysokhan, M., Dezfouli, A.M. et al. Nutritional Support of Crocin on Neurobehavioral Disabilities Induced by Cisplatin-Based Chemotherapy in Rats. Neurochem Res 49, 649–659 (2024). https://doi.org/10.1007/s11064-023-04059-8

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