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Requirement of Glycogenolysis for Uptake of Increased Extracellular K+ in Astrocytes: Potential Implications for K+ Homeostasis and Glycogen Usage in Brain

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Abstract

The importance of astrocytic K+ uptake for extracellular K+ ([K+]e) clearance during neuronal stimulation or pathophysiological conditions is increasingly acknowledged. It occurs by preferential stimulation of the astrocytic Na+,K+-ATPase, which has higher Km and Vmax values than its neuronal counterpart, at more highly increased [K+]e with additional support of the cotransporter NKCC1. Triggered by a recent DiNuzzo et al. paper, we used administration of the glycogenolysis inhibitor DAB to primary cultures of mouse astrocytes to determine whether K+ uptake required K+-stimulated glycogenolysis. KCl was increased by either 5 mM (stimulating only the Na+,K+-ATPase) or 10 mM (stimulating both transporters) in glucose-containing saline media prepared to become iso-osmotic after the addition. DAB completely inhibited both uptakes, the Na+,K+-ATPase-mediated by preventing Na+ uptake for stimulation of its intracellular Na+-activated site, and the NKCC1-mediated uptake by inhibition of depolarization- and L-channel-mediated Ca2+ uptake. Drugs inhibiting the signaling pathways involved in either of these processes also abolished K+ uptake. Assuming similar in vivo characteristics, partly supported by literature data, K+-stimulated astrocytic K+ uptake must discontinue after normalization of extracellular K+. This will allow Kir1.4-mediated release and reuptake by the less powerful neuronal Na+,K+-ATPase.

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References

  1. Macaulay N, Zeuthen T (2012) Glial K+ clearance and cell swelling: key roles for cotransporters and pumps. Neurochem Res 37:2299–2309

    Google Scholar 

  2. Ransom CB, Ransom BR, Sontheimer H (2000) Activity-dependent extracellular K+ accumulation in rat optic nerve: the role of glial and axonal Na+ pumps. J Physiol 522(Pt 3):427–442

    Article  PubMed  CAS  Google Scholar 

  3. Somjen GG, Kager H, Wadman WJ (2008) Computer simulations of neuron-glia interactions mediated by ion flux. J Comput Neurosci 25:349–365

    Article  PubMed  CAS  Google Scholar 

  4. Walz W (2000) Role of astrocytes in the clearance of excess extracellular potassium. Neurochem Int 36:291–300

    Article  PubMed  CAS  Google Scholar 

  5. Wang F, Smith NA, Xu Q et al (2012) Astrocytes modulate neural network activity by Ca2+-dependent uptake of extracellular K+. Sci Signal 5:ra26

    Article  PubMed  Google Scholar 

  6. Wang F, Xu Q, Wang W et al (2012) Bergmann glia modulate cerebellar Purkinje cell bistability via Ca2+-dependent K+ uptake. Proc Natl Acad Sci USA 109:7911–7916

    Article  PubMed  CAS  Google Scholar 

  7. Grisar T, Franck G, Schoffeniels E (1980) Glial control of neuronal excitability in mammals: II. Enzymatic evidence: two molecular forms of the Na+, K+-ATPase in brain. Neurochem Int 2C:311–320

    Article  PubMed  CAS  Google Scholar 

  8. Hajek I, Subbarao KV, Hertz L (1996) Acute and chronic effects of potassium and noradrenaline on Na+, K+-ATPase activity in cultured mouse neurons and astrocytes. Neurochem Int 28:335–342

    Article  PubMed  CAS  Google Scholar 

  9. Henn FA, Haljamae H, Hamberger A (1972) Glial cell function: active control of extracellular K+ concentration. Brain Res 43:437–443

    Article  PubMed  CAS  Google Scholar 

  10. Walz W (1992) Role of Na/K/Cl cotransport in astrocytes. Can J Physiol Pharmacol 70(Suppl):S260–S262

    Article  PubMed  CAS  Google Scholar 

  11. Walz W, Hertz L (1984) Intense furosemide-sensitive potassium accumulation in astrocytes in the presence of pathologically high extracellular potassium levels. J Cereb Blood Flow Metab 4:301–304

    Article  PubMed  CAS  Google Scholar 

  12. Dufour S, Dufour P, Chever O et al (2011) In vivo simultaneous intra- and extracellular potassium recordings using a micro-optrode. J Neurosci Methods 194:206–217

    Article  PubMed  CAS  Google Scholar 

  13. Bay V, Butt AM (2012) Relationship between glial potassium regulation and axon excitability: a role for glial Kir4.1 channels. Glia 60:651–660

    Article  PubMed  Google Scholar 

  14. Dinuzzo M, Mangia S, Maraviglia B et al (2012) The role of astrocytic glycogen in supporting the energetics of neuronal activity. Neurochem Res 37:2423–2438

    Google Scholar 

  15. Hof PR, Pascale E, Magistretti PJ (1988) K+ at concentrations reached in the extracellular space during neuronal activity promotes a Ca2+-dependent glycogen hydrolysis in mouse cerebral cortex. J Neurosci 8:1922–1928

    PubMed  CAS  Google Scholar 

  16. Bernard C (1878) Leçons sur les phénomènes de la vie communs aux animaux et aux végétaux, Tome 1. Edité par Albert Dastre. J.-B. Baillière et fils, Paris

    Google Scholar 

  17. Walz W, Hertz L (1982) Ouabain-sensitive and ouabain-resistant net uptake of potassium into astrocytes and neurons in primary cultures. J Neurochem 39:70–77

    Article  PubMed  CAS  Google Scholar 

  18. Sykova E (1992) K+ homeostasis in the ECS. In: Sykova E (ed) Ionic and volume changes in the microenvironment of nerve and receptor cells in progress in sensory physiology. Springer, Berlin, pp 7–26

    Chapter  Google Scholar 

  19. Epstein FH, Silva P (1985) Na-K-Cl cotransport in chloride-transporting epithelia. Ann N Y Acad Sci 456:187–197

    Article  PubMed  CAS  Google Scholar 

  20. Dawson DC (1987) Cellular mechanisms for K transport across epithelial cell layers. Semin Nephrol 7:185–192

    PubMed  CAS  Google Scholar 

  21. Kimelberg HK, Frangakis MV (1985) Furosemide- and bumetanide-sensitive ion transport and volume control in primary astrocyte cultures from rat brain. Brain Res 361:125–134

    Article  PubMed  CAS  Google Scholar 

  22. Walz W, Hinks EC (1986) A transmembrane sodium cycle in astrocytes. Brain Res 368:226–232

    Article  PubMed  CAS  Google Scholar 

  23. Tas PW, Massa PT, Kress HG et al (1987) Characterization of an Na+/K+/Cl− cotransport in primary cultures of rat astrocytes. Biochim Biophys Acta 903:411–416

    Article  PubMed  CAS  Google Scholar 

  24. Kanaka C, Ohno K, Okabe A et al (2001) The differential expression patterns of messenger RNAs encoding K-Cl cotransporters (KCC1, 2) and Na-K-2Cl cotransporter (NKCC1) in the rat nervous system. Neuroscience 104:933–946

    Article  PubMed  CAS  Google Scholar 

  25. Mikawa S, Wang C, Shu F et al (2002) Developmental changes in KCC1, KCC2 and NKCC1 mRNAs in the rat cerebellum. Brain Res Dev Brain Res 136:93–100

    Article  PubMed  CAS  Google Scholar 

  26. Deisz RA, Lehmann TN, Horn P et al (2011) Components of neuronal chloride transport in rat and human neocortex. J Physiol 589:1317–1347

    Article  PubMed  CAS  Google Scholar 

  27. Zhang LL, Delpire E, Vardi N (2007) NKCC1 does not accumulate chloride in developing retinal neurons. J Neurophysiol 98:266–277

    Article  PubMed  CAS  Google Scholar 

  28. Pedersen SF, O’Donnell ME, Anderson SE et al (2006) Physiology and pathophysiology of Na+/H+ exchange and Na+-K+-2Cl− cotransport in the heart, brain, and blood. Am J Physiol Regul Integr Comp Physiol 291:R1–R25

    Article  PubMed  CAS  Google Scholar 

  29. Subbarao KV, Stolzenburg JU, Hertz L (1995) Pharmacological characteristics of potassium-induced, glycogenolysis in astrocytes. Neurosci Lett 196:45–48

    Article  PubMed  CAS  Google Scholar 

  30. Cai L, Du T, Song D et al (2011) Astrocyte ERK phosphorylation precedes K+-induced swelling but follows hypotonicity-induced swelling. Neuropathology 31:250–264

    Article  PubMed  Google Scholar 

  31. Hertz L, Bender AS, Woodbury DM et al (1989) Potassium-stimulated calcium uptake in astrocytes and its potent inhibition by nimodipine. J Neurosci Res 22:209–215

    Article  PubMed  CAS  Google Scholar 

  32. Peng L, Du T, Xu J et al (2012) Adrenergic and V1-ergic agonists/antagonists affecting recovery from brain trauma in the lund project act on astrocytes. Curr Signal Transduct Ther 7:43–55

    Article  CAS  Google Scholar 

  33. MacVicar BA (1984) Voltage-dependent calcium channels in glial cells. Science 226:1345–1347

    Article  PubMed  CAS  Google Scholar 

  34. Duffy S, MacVicar BA (1994) Potassium-dependent calcium influx in acutely isolated hippocampal astrocytes. Neuroscience 61:51–61

    Article  PubMed  CAS  Google Scholar 

  35. Brown AM, Westenbroek RE, Catterall WA et al (2001) Axonal L-type Ca2+ channels and anoxic injury in rat CNS white matter. J Neurophysiol 85:900–911

    PubMed  CAS  Google Scholar 

  36. Cahoy JD, Emery B, Kaushal A et al (2008) A transcriptome database for astrocytes, neurons, and oligodendrocytes: a new resource for understanding brain development and function. J Neurosci 28:264–278

    Article  PubMed  CAS  Google Scholar 

  37. Barres BA, Koroshet WJ, Chun LL et al (1990) Ion channel expression by whiteatter glia: the type-1 astrocytes. Neuron 5:527–544

    Article  PubMed  CAS  Google Scholar 

  38. Lovatt D, Sonnewald U, Waagepetersen HS et al (2007) The transcriptome and metabolic gene signature of protoplasmic astrocytes in the adult murine cortex. J Neurosci 27:12255–12266

    Article  PubMed  CAS  Google Scholar 

  39. Westenbroek RE, Bausch SB, Lin RC et al (1998) Upregulation of L-type Ca2+ channels in reactive astrocytes after brain injury, hypomyelination, and ischemia. J Neurosci 18:2321–2334

    PubMed  CAS  Google Scholar 

  40. Peng L (2004) Transactivation in astrocytes as a novel mechanism of neuroprotection. In: Hertz L (ed) Non-neuronal cells of the nervous system: function and dysfunction. Elsevier, Amsterdam, pp 503–518

    Google Scholar 

  41. Mollenhauer HH, Morre DJ, Minnifield N (1992) Swelling response of golgi apparatus cisternae in cells treated with monensin is reduced by cell injury. Cell Biol Int Rep 16:217–220

    Article  PubMed  CAS  Google Scholar 

  42. Gautron S, Dos Santos G, Pinto-Henrique D et al (1992) The glial voltage-gated sodium channel: cell- and tissue-specific mRNA expression. Proc Natl Acad Sci USA 89:7272–7276

    Article  PubMed  CAS  Google Scholar 

  43. Black JA, Dib-Hajj S, Cohen S et al (1998) Glial cells have heart: rH1 Na+ channel mRNA and protein in spinal cord astrocytes. Glia 23:200–208

    Article  PubMed  CAS  Google Scholar 

  44. Watanabe E, Fujikawa A, Matsunaga H et al (2000) Nav2/NaG channel is involved in control of salt-intake behavior in the CNS. J Neurosci 20:7743–7751

    PubMed  CAS  Google Scholar 

  45. Ogata N, Ohishi Y (2002) Molecular diversity of structure and function of the voltage-gated Na+ channels. Jpn J Pharmacol 88:365–377

    Article  PubMed  CAS  Google Scholar 

  46. Shimizu H, Watanabe E, Hiyama TY et al (2007) Glial Nax channels control lactate signaling to neurons for brain Na+ sensing. Neuron 54:59–72

    Article  PubMed  CAS  Google Scholar 

  47. Mobasheri A, Barrett-Jolley R, Shakibaei M et al (2005) Enigmatic roles of the epithelial sodium channel (ENaC) in articular chondrocytes and osteoblasts: mechanotransduction, sodium transport or extracellular sodium sensing? In: Kamkin A, Kiseleva I (eds) Mechanosensitivity in cells and tissues. Academia, Moscow, pp 452–464

    Google Scholar 

  48. Reinhard L, Tidow H, Clausen MJ et al (2012) Na+, K+-ATPase as a docking station: protein–protein complexes of the Na+, K+-ATPase. Cell Mol Life Sci. doi:10.1007/s00018-012-1039-9

  49. Xue Z, Li B, Gu L et al (2010) Increased Na, K-ATPase alpha2 isoform gene expression by ammonia in astrocytes and in brain in vivo. Neurochem Int 57:395–403

    Article  PubMed  CAS  Google Scholar 

  50. Zhang L, Zhang Z, Guo H et al (2008) Na+/K+-ATPase-mediated signal transduction and Na+/K+-ATPase regulation. Fundam Clin Pharmacol 22:615–621

    Article  PubMed  CAS  Google Scholar 

  51. Juurlink BHJ, Hertz L (1992) Astrocytes. In: Boulton AA, Baker GB, Walz W (eds) Neuromethods in cell cultures. Humana Clifton, New York, pp 269–321; Internet availability in Springer Protocols, Neuroscience

  52. Hertz L, Peng L, Lai JC (1998) Functional studies in cultured astrocytes. Methods 16:293–310

    Article  PubMed  CAS  Google Scholar 

  53. Li B, Du T, Li H et al (2008) Signalling pathways for transactivation by dexmedetomidine of epidermal growth factor receptors in astrocytes and its paracrine effect on neurons. Br J Pharmacol 154:191–203

    Article  PubMed  CAS  Google Scholar 

  54. Hertz L, Juurlink BHJ, Szuchet S (1985) Cell cultures. In: Lajtha A (ed) Handbook of neurochemistry. Plenum, New York, pp 603–661

    Google Scholar 

  55. Meier E, Hertz L, Schousboe A (1991) Neurotransmitters as developmental signals. Neurochem Int 19:1–15

    Article  CAS  Google Scholar 

  56. Lange SC, Bak LK, Waagepetersen HS et al (2012) Primary cultures of astrocytes: their value in understanding astrocytes in health and disease. Neurochem Res 37:2569–2588

    Article  PubMed  CAS  Google Scholar 

  57. Li B, Dong L, Wang B et al (2012) Cell type-specific gene expression and editing responses to chronic fluoxetine treatment in the in vivo mouse brain and their relevance for stress-induced anhedonia. Neurochem Res 37:2380–2495

    Google Scholar 

  58. Li B, Hertz L, Peng L (2012b) Aralar mRNA and protein levels in neurons and astrocytes freshly isolated from young and adult mouse brain and in maturing cultured astrocytes. Neurochem Int 61:1325–1332

    Google Scholar 

  59. Song D, Li B, Yan EZ et al (2012) Chronic treatment with anti-bipolar drugs causes intracellular alkalinization in astrocytes, altering their functions. Neurochem Res 37:2524–2540

    Article  PubMed  CAS  Google Scholar 

  60. Deshmukh HS, Case LM, Wesselkamper SC et al (2005) Metalloproteinases mediate mucin 5AC expression by epidermal growth factor receptor activation. Am J Respir Crit Care Med 171:305–314

    Article  PubMed  Google Scholar 

  61. Laskay G, Varhelyi T, Dale RE et al (1995) Role of interleukin-3 in the regulation of intracellular K+ homeostasis in cultured murine hematopoietic cells. Biochem Biophys Res Commun 214:348–353

    Article  PubMed  CAS  Google Scholar 

  62. Andersson B, Janson V, Behnam-Motlagh P et al (2006) Induction of apoptosis by intracellular potassium ion depletion: using the fluorescent dye PBFI in a 96-well plate method in cultured lung cancer cells. Toxicol In Vitro 20:986–994

    Article  PubMed  CAS  Google Scholar 

  63. Yates SL, Fluhler EN, Lippiello PM (1992) Advances in the use of the fluorescent probe fura-2 for the estimation of intrasynaptosomal calcium. J Neurosci Res 32:255–260

    Article  PubMed  CAS  Google Scholar 

  64. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254

    Article  PubMed  CAS  Google Scholar 

  65. Sickmann HM, Schousboe A, Fosgerau K et al (2005) Compartmentation of lactate originating from glycogen and glucose in cultured astrocytes. Neurochem Res 30:1295–1304

    Article  PubMed  CAS  Google Scholar 

  66. Gibbs M, Lloyd H, Santa T et al (2007) Glycogen is a preferred glutamate precursor during learning in 1-day-old chick: biochemical and behavioral evidence. J Neurosci Res 85:3326–3333

    Article  PubMed  CAS  Google Scholar 

  67. Obel LF, Muller MS, Walls AB et al (2012) Brain glycogen-new perspectives on its metabolic function and regulation at the subcellular level. Front Neuroenergetics 4:3

    PubMed  CAS  Google Scholar 

  68. Ma D, Wang J, Zhao Y et al (2012) Inhibition of glycogen phosphorylation induces changes in cellular proteome and signaling pathways in MIA pancreatic cancer cells. Pancreas 41:397–408

    Article  PubMed  CAS  Google Scholar 

  69. Sickmann HM, Waagepetersen HS, Schousboe A et al (2012) Brain glycogen and its role in supporting glutamate and GABA homeostasis in a type 2 diabetes rat model. Neurochem Int 60:267–275

    Article  PubMed  CAS  Google Scholar 

  70. Obel LF, Andersen KM, Bar LK et al (2012) Effects of adrenergic agents on intracellular Ca2+ homeostasis and metabolism of glucose in astrocytes with an emphasis on pyruvate carboxylation, oxidative decarboxylation and recycling: implications for glutamate neurotransmission and excitotoxicity. Neurotox Res 21:405–417

    Article  PubMed  CAS  Google Scholar 

  71. Kaufman E, Driscoll B (1992) Carbon dioxide fixation in neuronal and astroglial cells in culture. J Neurochem 58:258–262

    Article  PubMed  CAS  Google Scholar 

  72. Öz G, Tesfaye N, Kumar A et al (2012) Brain glycogen content and metabolism in subjects with type 1 diabetes and hypoglycemia unawareness. J Cereb Blood Flow Metab 32:256–263

    Article  PubMed  Google Scholar 

  73. Hertz L (2011) Astrocytic energy metabolism and glutamate formation—relevance for 13C-NMR spectroscopy and importance of cytosolic/mitochondrial trafficking. Magn Reson Imaging 29:1319–1329

    Article  PubMed  CAS  Google Scholar 

  74. Zhao Z, Hertz L, Code WE (1996) Effects of benzodiazepines on potassium-induced increase in free cytosolic calcium concentration in astrocytes: interactions with nifedipine and the peripheral-type benzodiazepine antagonist PK 11195. Can J Physiol Pharmacol 74:273–277

    Article  PubMed  CAS  Google Scholar 

  75. Ozawa E (2011) Regulation of phosphorylase kinase by low concentrations of Ca ions upon muscle contraction: the connection between metabolism and muscle contraction and the connection between muscle physiology and Ca-dependent signal transduction. Proc Jpn Acad Ser B Phys Biol Sci 87:486–508

    Article  PubMed  CAS  Google Scholar 

  76. Scemes E, Spray DC (2012) Extracellular K+ and astrocyte signaling via connexin and pannexin channels. Neurochem Res 37:2310–2316

    Article  PubMed  CAS  Google Scholar 

  77. Magistretti PJ, Morrison JH, Shoemaker WJ et al (1983) Effect of 6-hydroxydopamine lesions on norepinephrine-induced [3H]glycogen hydrolysis in mouse cortical slices. Brain Res 261:159–166

    Article  PubMed  CAS  Google Scholar 

  78. Cambray-Deakin M, Pearce B, Morrow C et al (1988) Effects of neurotransmitters on astrocyte glycogen stores in vitro. J Neurochem 51:1852–1857

    Article  PubMed  CAS  Google Scholar 

  79. Subbarao KV, Hertz L (1990) Noradrenaline induced stimulation of oxidative metabolism in astrocytes but not in neurons in primary cultures. Brain Res 527:346–349

    Article  PubMed  CAS  Google Scholar 

  80. Gibbs ME, Anderson DG, Hertz L (2006) Inhibition of glycogenolysis in astrocytes interrupts memory consolidation in young chickens. Glia 54:214–222

    Article  PubMed  Google Scholar 

  81. Hertz L, Gibbs ME (2009) What learning in day-old chickens can teach a neurochemist: focus on astrocyte metabolism. J Neurochem 109(Suppl 1):10–16

    Article  PubMed  CAS  Google Scholar 

  82. Suzuki A, Stern SA, Bozdaqi O et al (2011) Astrocyte-neuron lactate transport is required for long-term memory formation. Cell 144:810–823

    Article  PubMed  CAS  Google Scholar 

  83. Gibbs ME, Hutchinson DS (2012) Rapid turnover of glycogen in memory formation. Neurochem Res 37:2456–2463

    Article  PubMed  CAS  Google Scholar 

  84. Ito M, Spicer SS, Schulte BA (1994) Histochemical detection of glycogen and glycoconjugates in the inner ear with modified concanavalin A-horseradish peroxidase procedures. Histochem J 26:437–446

    Article  PubMed  CAS  Google Scholar 

  85. Tseng YC, Huang CJ, Chang JC et al (2007) Glycogen phosophorylase in glycogen-rich cells is involved in the energy supply for ion regulation in fish gill epithelia. Am J Physiol Requl Inteqr Comp Physiol 293:R482–R491

    Article  CAS  Google Scholar 

  86. Tipsmark CK, Madsen SS, Borski RJ (2004) Effect of salinity on expression of branchial ion transporters in striped bass (Morone saxatilis). J Exp Zool A Comp Exp Biol 301:979–991

    Article  PubMed  Google Scholar 

  87. Chu HQ, Huang XW, Xiong H et al (2006) Role of ion channel Na-K-2Cl and alpha2 Na, K-ATPase in cochlear potassium cycling and auditory function. Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 41:692–697

    PubMed  Google Scholar 

  88. Kajimura S, Hirano T, Moriyama S et al (2004) Changes in plasma concentrations of immunoreactive ouabain in the tilapia in response to changing salinity: is ouabain a hormone in fish? Gen Comp Endocrinol 135:90–99

    Article  PubMed  CAS  Google Scholar 

  89. Larre I, Lazaro A, Contreras RG, Balda MS et al (2010) Ouabain modulates epithelial cell tight junction. Proc Natl Acad Sci USA 107:11387–11392

    Article  PubMed  CAS  Google Scholar 

  90. Forshammar J, Block L, Lundborg C et al (2011) Naloxone and ouabain in ultralow concentrations restore Na+/K+-ATPase and cytoskeleton in lipopolysaccharide-treated astrocytes. J Biol Chem 286:31586–31597

    Article  PubMed  CAS  Google Scholar 

  91. Swanson RA (1992) Physiologic coupling of glial glycogen metabolism to neuronal activity in brain. Can J Physiol Pharmacol 70(Suppl):S138–S144

    Article  PubMed  CAS  Google Scholar 

  92. Carmignoto G, Pasti L, Pozzan T (1998) On the role of voltage-dependent calcium channels in calcium signaling of astrocytes in situ. J Neurosci 18:4637–4645

    PubMed  CAS  Google Scholar 

  93. Choi HB, Gordon GR, Zhou N et al (2012) Metabolic communication between astrocytes and neurons via bicarbonate-responsive soluble adenylyl cyclase. Neuron 75:1094–1104

    Article  PubMed  CAS  Google Scholar 

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Acknowledgments

The authors of DiNuzzo et al. [14] are cordially thanked for their contemplative suggestion that glycogenolysis may be involved in astrocytic K+ uptake, without which this study would not have been undertaken. Our study was supported by Grant No. 31171036 to L. P. from the National Natural Science Foundation of China.

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  1. Zhanxia Xue

    Present address: Department of Pharmacology, University of Hebei North University, Zhangjiakou, People’s Republic of China

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  1. Department of Clinical Pharmacology, China Medical University, No. 92 Beier Road, Heping District, Shenyang, People’s Republic of China

    Junnan Xu, Dan Song, Zhanxia Xue, Li Gu, Leif Hertz & Liang Peng

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Correspondence to Liang Peng.

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Junnan Xu and Dan Song contributed equally to this work.

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Xu, J., Song, D., Xue, Z. et al. Requirement of Glycogenolysis for Uptake of Increased Extracellular K+ in Astrocytes: Potential Implications for K+ Homeostasis and Glycogen Usage in Brain. Neurochem Res 38, 472–485 (2013). https://doi.org/10.1007/s11064-012-0938-3

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