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Expression Pattern and Splicing Function of Mouse ZNF265

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Abstract

ZNF265 is a newly identified arginine/serine-rich (SR) protein and has two transcript isoforms (ZNF265-1 and ZNF265-2) that autoregulate between each other. Previous studies have shown that ZNF265 regulates the Tra2β isoform splicing. Here, we demonstrate that two ZNF-265 transcript isoforms are expressed in various mouse tissues and that ZNF265-1 is a major isoform. The ZNF265-1 protein level in the cerebral cortex is significantly lower in relative to other tissues. The recombinant proteins of both isoforms are nuclear, in consistent with its functions as pre-mRNA splicing regulators. Splicing analysis with GluR-B and SMN2 minigenes demonstrates that ZNF265-1 inhibits the Flop exon and exon 7 usages in the splicing of two minigenes, respectively. The regulation of GluR-B and SMN2 pre-mRNA splicing by ZNF265 implies this newly identified SR protein may play important roles in maintaining normal neuronal function and SMA pathogenesis.

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Acknowledgments

We thank Dr. Brunhilde Wirth (Bonn University, Germany) for the gift of the plasmids of SMN2 minigene. This work was supported by National Foundation of Natural Sciences of China (#30470383, #30470535).

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Authors and Affiliations

  1. State Key Laboratory of Medical Neurobiology and Laboratory of Genomic Physiology, Brain Research Center, Fudan University, Shanghai, 200032, P.R. China

    Jing Li, Xian-hua Chen, Ping-jie Xiao, Li Li, Wan-min Lin, Jia Huang & Ping Xu

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  1. Jing Li
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  2. Xian-hua Chen
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  3. Ping-jie Xiao
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  4. Li Li
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  6. Jia Huang
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  7. Ping Xu
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Correspondence to Xian-hua Chen or Ping Xu.

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Li, J., Chen, Xh., Xiao, Pj. et al. Expression Pattern and Splicing Function of Mouse ZNF265. Neurochem Res 33, 483–489 (2008). https://doi.org/10.1007/s11064-007-9461-3

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  • DOI: https://doi.org/10.1007/s11064-007-9461-3

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