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Alterations in the Development of Rat Cerebellum and Impaired Behavior of Juvenile Rats after Neonatal 6-OHDA Treatment

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Abstract

The effects of neonatal systemic administration of the neurotoxin 6-hydroxydopamine (6-OHDA) on cerebellum development and behavior were studied in juvenile rats. The methods employed were immunohistochemistry, in situ hybridization, ligand binding, and behavioral testing. The results revealed, for the first time, that 6-OHDA treatment alters Bergmann glial cells and reduced the expression GABAA receptor subtypes α1 and α6 especially in granule cells. The Bergmann glial cells were abnormally located and structurally different (e.g., no intimate associations with Purkinje cells). Significant microglial activation was also observed. The animals showed impairment in behavior, especially in their orientation to a novel environment. Recent data on neuron–glia interactions support the conclusion that the observed structural changes in Bergmann glia and granular neurons disrupted the normal functioning of the Purkinje cells which then in turn resulted in the impaired sensory-motor coordination at least in juvenile rats. This paper is a summary of previously published work and some recent data in this field obtained at our laboratory.

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Authors and Affiliations

  1. Deprtment of Physiology, Medical School, University of Tampere, FIN-33014, Finland

    I. Djatchkova-Podkletnova

  2. Department of Mental Health and Alcohol Research, National Public Health Institute (KTL), POB 3300251, Helsinki, Finland

    H. Alho

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  1. I. Djatchkova-Podkletnova
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Correspondence to I. Djatchkova-Podkletnova.

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Special issue dedicated to Dr. Simo S. Oja

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Djatchkova-Podkletnova, I., Alho, H. Alterations in the Development of Rat Cerebellum and Impaired Behavior of Juvenile Rats after Neonatal 6-OHDA Treatment. Neurochem Res 30, 1599–1605 (2005). https://doi.org/10.1007/s11064-005-8838-4

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