The effects of intracerebroventricular administration of fragment (25–35) of β-amyloid peptide [Aβ(25–35)] on cell proliferation in the subventricular zone of the dentate gyrus of the hippocampus in adult rats were analyzed. Animals received doses of 15 nmol of pre-aggregated Aβ(25–35) or the Aβ(35–25) control peptide, or solvent (sterile water) into the lateral ventricles. On post-injection days 1–5, rats received intraperitoneal injections of the thymidine analog 5-bromo-2’-deoxyuridine (BrdU). BrdU incorporated into DNA was detected immunohistochemically on frontal brain sections six and 12 days after peptide administration. At six days, the numbers of BrdU-containing cells in the subventricular zone showed no differences between the study groups. At 12 days, the total number of BrdU-positive cells decreased significantly in all study groups. At the same time, the number of labeled cells in rats given Aβ(25–35) was significantly greater in this brain zone than in animals given water or the control peptide. Thus, Aβ(25–35) significantly increased cell proliferation in the subventricular zone after intracerebroventricular administration.
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Translated from Morfologiya, Vol. 135, No. 1, pp. 13–16, January–February, 2009.
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Stepanichev, M.Y., Moiseeva, Y.V., Lazareva, N.A. et al. Changes in Cell Proliferation in the Subventricular Zone of the Brain in Adult Rats Given β-Amyloid Peptide (25–35). Neurosci Behav Physi 40, 123–126 (2010). https://doi.org/10.1007/s11055-009-9232-5
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DOI: https://doi.org/10.1007/s11055-009-9232-5