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In vitro effects of cisplatin-functionalized silica nanoparticles on chondrocytes

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Abstract

In this study, we evaluated the combined effect of a known toxic molecule, cisplatin, in combination with relatively nontoxic nanoparticles, amorphous fumed silica, on chondrocyte cells. Cisplatin was attached to silica nanoparticles using aminopropyltriethoxy silane as a linker molecule, and characterized in terms of size, shape, specific surface area, as well as the dissolution of cisplatin from the silica surface. The primary particle diameter of the as-received silica nanoparticles ranged from 7.1 to 61 nm, estimated from measurements of specific surface area, and the primary particles were aggregated. The effects of cisplatin-functionalized silica particles with different specific surface areas (41, 85, 202, 237, and 297 m2/g) were compared in vitro on chondrocytes, the parenchymal cell of hyaline cartilage. The results show that adverse effects on cell function, as evidenced by reduced metabolic activity measured by the MTT assay and increased membrane permeability observed using the Live/Dead stain, can be correlated with specific surface area of the silica. Cisplatin-functionalized silica nanoparticles with the highest specific surface area incited the greatest response, which was almost equivalent to that induced by free cisplatin. This result suggests the importance of particle specific surface area in interactions between cells and surface-functionalized nanomaterials.

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Acknowledgments

The authors acknowledge with thanks Dr. Wen An Chiou, (NISPLab, College Park, University of Maryland), for assistance in TEM, and Tim Maugel, (Laboratory for Biological Ultrastructure, University of Maryland) for assistance in thin sectioning. The authors also thank Dr. Karen Gaskell (Department of Chemistry and Biochemistry, University of Maryland) for assistance with XPS measurements. We acknowledge the support of the Maryland NanoCenter and its NispLab. The NispLab and XPS facilities are supported in part by the National Science Foundation as MRSEC Shared Experimental Facilities. This study was supported by National Institute of Standards and Technology (NIST) (grant no—60NANB6D6169).

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Authors and Affiliations

  1. Department of Chemical and Biomolecular Engineering, University of Maryland, 1223C Chemical & Nuclear Engineering Building, College Park, MD, 20742, USA

    Tridib Kumar Bhowmick, Diana Yoon & Sheryl Ehrman

  2. Fischell Department of Bioengineering, University of Maryland, College Park, MD, 20742, USA

    Minal Patel & John Fisher

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  1. Tridib Kumar Bhowmick
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  2. Diana Yoon
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  3. Minal Patel
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  4. John Fisher
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Correspondence to Sheryl Ehrman.

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Bhowmick, T.K., Yoon, D., Patel, M. et al. In vitro effects of cisplatin-functionalized silica nanoparticles on chondrocytes. J Nanopart Res 12, 2757–2770 (2010). https://doi.org/10.1007/s11051-010-9849-x

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  • DOI: https://doi.org/10.1007/s11051-010-9849-x

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