Skip to main content

Advertisement

SpringerLink
Log in

Gelatin–Chitosan composite capped gold nanoparticles: a matrix for the growth of hydroxyapatite

  • Research Paper
  • Published:
Journal of Nanoparticle Research Aims and scope Submit manuscript

Abstract

Growth of hydroxyapatite (HA) on gelatin–chitosan composite capped gold nanoparticles is presented for the first time by employing wet precipitation methods and we obtained good yields of HA. Fourier transform infrared spectroscopy (FTIR) spectrum has shown the characteristic bands of phosphate groups in the HA. Scanning electron microscopy (SEM) pictures have shown spherical nanoparticles with the size in the range of 70–250 nm, whereas ≥2–50 nm sized particles were visualized in high resolution transmission electron microscopy (HR-TEM). X-ray diffraction (XRD) spectrum has shown Bragg reflections which are comparable with the HA. Energy dispersive X-ray (EDX) studies have confirmed calcium/phosphate stoichiometric ratio of HA. The thermogravimetric analysis (TGA) has shown about 74% of inorganic crystals in the nanocomposite formed. These results have revealed that gelatin–chitosan capped gold nanoparticles, acted as a matrix for the growth of HA.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

References

  • Aryal S, Bahadur R, Bhattarai SR, Prabu P, Kim HY (2006) Immobilization of collagen on gold nanoparticles: preparation, characterization, and hydroxyapatite growth. J Mater Chem 16:4642–4648

    Article  CAS  Google Scholar 

  • Borginon H (1967) Photographic properties of the gelatin macromolecule. J Photogr Sci 15:207–214

    CAS  Google Scholar 

  • Chang MC, Ikoma T, Kikuchi M, Tanaka J (2001) Preparation of a porous hydroxyapatite/collagen nanocomposite using glutaraldehyde as a crosslinkage agent. J Mater Sci Lett 20:1199–1201

    Article  CAS  Google Scholar 

  • David L, Argenta L, Fisher D (2005) Hydroxyapatite cement in pediatric craniofacial reconstruction. J Craniofac Surg 16:129–133

    Article  PubMed  Google Scholar 

  • Du C, Cui FZ, Zhu XD, de Groot K (1999) Three-dimensional nano-HAp/collagen matrix loading with osteogenic cells in organ culture. J Biomed Mater Res 44:407–415

    Article  PubMed  CAS  Google Scholar 

  • Dufresne A, Cavaille JY, Dupeyre D, Garcia-Ramirez M, Romero J (1999) Morphology, phase continuity and mechanical behaviour of polyamide 6 chitosan blends. Polymer 40:1657–1666

    Article  CAS  Google Scholar 

  • Elcin AE, Elcin YM, Pappas GD (1998) Neural tissue engineering: adrenal chromaffin cell attachment and viability on chitosan scaffolds. Neurol Res 20:648–654

    PubMed  CAS  Google Scholar 

  • Esumi K, Takei N, Yoshimura T (2003) Antioxidant-potentiality of gold–chitosan nanocomposites. Colloids Surf B Biointerfaces 32:117–123

    Article  CAS  Google Scholar 

  • Geiger M, Li RH, Friess W (2003) Collagen sponges for bone regeneration with rhBMP-2. Adv Drug Deliv Rev 55:1613–1629

    Article  PubMed  CAS  Google Scholar 

  • Gineste L, Gineste M, Ranz X, Ellefterion A, Guilhem A, Rouquet N, Frayssinet P (1999) Degradation of hydroxylapatite, fluorapatite, and fluorhydroxyapatite coatings of dental implants in dogs. J Biomed Mater Res 48:224–234

    Article  PubMed  CAS  Google Scholar 

  • Huang J, Best SM, Bonfield W, Brooks RA, Rushton N, Jayasinghe SN, Edirisinghe MJ (2004) In vitro assessment of the biological response to nano-sized hydroxyapatite. J Mater Sci Mater Med 15:441–445

    Article  PubMed  CAS  Google Scholar 

  • Khor E, Lim LY (2003) Implantable applications of chitin and chitosan. Biomaterials 24:2339–2349

    Article  PubMed  CAS  Google Scholar 

  • Kometani N, Tsubonishi M, Fujita T, Asami K, Yonezawa Y (2001) Preparation and optical absorption spectra of dye-coated Au, Ag, and Au/Ag colloidal nanoparticles in aqueous solutions and in alternate assemblies. Langmuir 17:578–580

    Article  CAS  Google Scholar 

  • Liu Q, de Wijn JR, van Blitterswijk CA (1998) A study on the grafting reaction of isocyanates with hydroxyapatite particles. J Biomed Mater Res 40:358–364

    Article  PubMed  CAS  Google Scholar 

  • Ma PX, Langer R (1995) In: Mikos AG et al (eds) Polymers in medicine and pharmacy. MRS, Pittsburgh, pp 99–104

  • Manna A, Chen PL, Akiyama H, Wei TX, Tamada K, Knoll W (2003) Optimized photoisomerization on gold nanoparticles capped by unsymmetrical azobenzene disulfides. Chem Mater 15:20–28

    Article  CAS  Google Scholar 

  • Mao JS, Zhao LG, Yin YJ, Yao KD (2003) Structure and properties of bilayer chitosan–gelatin scaffolds. Biomaterials 24:1067–1074

    Article  PubMed  CAS  Google Scholar 

  • Mikos AG, Thorsen AJ, Czerwonka LA, Bao Y, Langer R, Winslow DN, Vacanti JP (1994) Preparation and characterization of poly(L-lactic acid) foams. Polymer 35:1068–1077

    Article  CAS  Google Scholar 

  • Misra TK, Chen TS, Liu CY (2006) Phase transfer of gold nanoparticles from aqueous to organic solution containing resorcinarene. J Colloid Interface Sci 297:584–588

    Article  PubMed  CAS  Google Scholar 

  • Mochizuki A, Sato Y, Ogawara H, Yamashita S (1989) Pervaporation separation of water/ethanol mixtures through polysaccharide membranes. II. The permselectivity of chitosan membrane. J Appl Polym Sci 37(12):3375−3384

    Article  CAS  Google Scholar 

  • Murphy CJ, San TK, Gole AM, Orendorff CJ, Gao JX, Gou L, Hunyadi SE, Li T (2005) Anisotropic metal nanoparticles: synthesis, assembly, and optical applications. J Phys Chem B 109:13857–13870

    Article  PubMed  CAS  Google Scholar 

  • Park YM, Ryu SC, Yoon SY, Stevens R, Park HC (2008) Preparation of whisker-shaped hydroxyapatite/β-tricalcium phosphate composite. Mater Chem Phys, doi:10.1016/j.matchemphys.2007.12.013

  • Rezania A., Healy KE (1999) Biomimetic peptide surfaces that regulate adhesion, spreading, cytoskeletal organization, and mineralization of the matrix deposited by osteoblast-like cells. Biotechnol Prog 15:19–32

    Article  PubMed  CAS  Google Scholar 

  • Rose F., Oreffo ROC, Nf (2002) Bone tissue engineering: hope vs hype. Biochem Biophys Res Commun 292:1–7

    Article  PubMed  CAS  Google Scholar 

  • Shea LD, Wang D, Franceschi RT, Mooney DJ (2000) Engineered bone development from a pre-osteoblast cell line on three-dimensional scaffolds. Tissue Eng 6:605–617

    Article  PubMed  CAS  Google Scholar 

  • Su CH, Wu PL, Yeh CS (2003) Sonochemical synthesis of well-dispersed gold nanoparticles at the ice temperature. J Phys Chem B 107:14240–14243

    Article  CAS  Google Scholar 

  • Suh JKF, Matthew HWT (2000) Application of chitosan-based polysaccharide biomaterials in cartilage tissue engineering: a review. Biomaterials 21:2589–2598

    Article  PubMed  CAS  Google Scholar 

  • Sun YG, Xia YN (2002) Shape-controlled synthesis of gold and silver nanoparticles. Science 298:2176–2179

    Article  PubMed  ADS  CAS  Google Scholar 

  • Yao CH, Sun JS, Lin FH, Liao CJ, Huang CW (1996) Biological effects and cytotoxicity of tricalcium phosphate and formaldehyde cross-linked gelatin composite. Mater Chem Phys 45:6–14

    Article  CAS  Google Scholar 

  • Zhao F, Grayson WL, Ma T, Bunnell B, Lu WW (2006) Effects of hydroxyapatite in 3-D chitosan–gelatin polymer network on human mesenchymal stem cell construct development. Biomaterials 27:1859–1867

    Article  PubMed  CAS  Google Scholar 

  • Zhao F, Yin YJ, Lu WW, Leong JC, Zhang WJ, Zhang JY, Zhang MF, Yao KD (2002) Preparation and histological evaluation of biomimetic three-dimensional hydroxyapatite/chitosan–gelatin network composite scaffolds. Biomaterials 23:3227–3234

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Bio-products Lab, Central Leather Research Institute, Adyar, Chennai, 600020, India

    S. S. Liji Sobhana, J. Sundaraseelan, S. Sekar, T. P. Sastry & A. B. Mandal

Authors
  1. S. S. Liji Sobhana
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. Sundaraseelan
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. S. Sekar
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. T. P. Sastry
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. A. B. Mandal
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to T. P. Sastry or A. B. Mandal.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sobhana, S.S.L., Sundaraseelan, J., Sekar, S. et al. Gelatin–Chitosan composite capped gold nanoparticles: a matrix for the growth of hydroxyapatite. J Nanopart Res 11, 333–340 (2009). https://doi.org/10.1007/s11051-008-9385-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11051-008-9385-0

Keywords

Search

Navigation