Skip to main content
SpringerLink
Log in

Morphological and Inter Simple Sequence Repeat (ISSR) Markers Analyses of Corynespora cassiicola Isolates from Rubber Plantations in Malaysia

  • Published:
Mycopathologia Aims and scope Submit manuscript

Abstract

Morphological features and Inter Simple Sequence Repeat (ISSR) polymorphism were employed to analyse 21 Corynespora cassiicola isolates obtained from a number of Hevea clones grown in rubber plantations in Malaysia. The C. cassiicola isolates used in this study were collected from several states in Malaysia from 1998 to 2005. The morphology of the isolates was characteristic of that previously described for C. cassiicola. Variations in colony and conidial morphology were observed not only among isolates but also within a single isolate with no inclination to either clonal or geographical origin of the isolates. ISSR analysis delineated the isolates into two distinct clusters. The dendrogram created from UPGMA analysis based on Nei and Li’s coefficient (calculated from the binary matrix data of 106 amplified DNA bands generated from 8 ISSR primers) showed that cluster 1 encompasses 12 isolates from the states of Johor and Selangor (this cluster was further split into 2 sub clusters (1A, 1B), sub cluster 1B consists of a unique isolate, CKT05D); while cluster 2 comprises of 9 isolates that were obtained from the other states. Detached leaf assay performed on selected Hevea clones showed that the pathogenicity of representative isolates from cluster 1 (with the exception of CKT05D) resembled that of race 1; and isolates in cluster 2 showed pathogenicity similar to race 2 of the fungus that was previously identified in Malaysia. The isolate CKT05D from sub cluster 1B showed pathogenicity dissimilar to either race 1 or race 2.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

Abbreviations

PDI:

Percent disease intensity

CMI:

Commonwealth Mycology Institute

UBC:

University of British Colombia

References

  1. Farr DF, Rossman AY, Palm ME, McCray EB. Fungal databases. Systematic Botany & Mycology Laboratory, ARS, USDA. Retrieved Dec 13, 2007, from http://nt.ars-grin.gov/fungaldatabases/.

  2. Deighton FC. Preliminary list of fungi and diseases of plants in Sierra Leone. Kew Bull. 1936;7:397–424.

    Google Scholar 

  3. Ramakrishnan TS, Pillay R. Leaf spot of rubber caused by Corynespora cassiicola (Berk. & Curt.). Wei Rubber Bd Bull. 1961;5:32–5.

    Google Scholar 

  4. Newsam S. Pathology division report. Rubber Res Inst Malaysia 1961;63–70.

  5. Awoderu VA. New leaf spot of para rubber (Hevea brasiliensis) in Nigeria. Plant Dis Reptr. 1969;53(5):406–8.

    Google Scholar 

  6. Teoh CH. Corynespora leaf fall of Hevea in West Java. Malaysian Plant Protection Society. Newsletter. 1983;7(2):12–3.

    Google Scholar 

  7. Junqeuira NTV, Gasparotto L, Moraes VHF, Silva HM, Lim TM. New diseases caused by virus, fungi and also bacterium on rubber from Brazil and their impact on international quarantine. In: Proceeding of the regional conference on plant quarantine support for agricultural development, Kuala Lumpur, Malaysia, 10–12 December 1985. p. 253–60.

  8. Liyanage AS, Jayasinghe CK, Liyanage NIS, Jayaratne AHR. Corynespora leaf spot disease of rubber (Hevea brasiliensis)––A new report. J Rubber Res Inst Sri Lanka. 1986;65:47–50.

    Google Scholar 

  9. Pongthep K. Corynespora disease of Hevea in Thailand. IRRDB’s symposium on pathology of Hevea in Chieng Mai, Thailand, 2–3rd Nov. 1987.

  10. Rahman MA. Diseases of Hevea brasiliensis in Bangladesh. Bano Biggyyan Patrika. 1988;17:73–9.

    Google Scholar 

  11. Dung PT, Hoan NT. Corynespora leaf fall on rubber in Vietnam, a New record. In: Proceeding of IRRDB Symposium 1999. Hainan Publishing House. p. 273–5.

  12. Jinji P, Zhang X, Qi Y, Xie Y, Zhang H, Zhang H. First record of Corynespora leaf fall disease of Hevea rubber tree in China. Aust Plant Dis Notes. 2007;2:35–6. doi:10.1071/DN07017.

    Google Scholar 

  13. Tan AM, Lo TP, Vadivel G, Bachik MS, Yoon KP. Survey of major leaf diseases of rubber in Peninsular Malaysia. RRIM Planters’ Bull. 1992;211:51–62.

    Google Scholar 

  14. Jayashinghe CK, Silva WPK. Current status of Corynespora leaf fall in Sri Lanka. In: Proceeding workshop on Corynespora leaf fall disease of Hevea rubber, 16–17 December 1996, Medan, Indonesia. p. 15–9.

  15. Sinulingga W, Suwarto, Soepena H. Current status of Corynespora leaf fall in Indonesia. In: Proceeding workshop on Corynespora leaf fall disease of Hevea rubber, 16–17 December 1996, Medan, Indonesia. p. 29–36.

  16. Breton F, D’Auzac J, Garcia D, Sanier C, Eschbach JM. Recent research on Corynespora cassiicola/Hevea brasiliensis interaction. In: Proceeding workshop on Corynespora leaf fall disease of Hevea rubber, 16–17 December 1996, Medan, Indonesia. p. 49–78.

  17. Shamsul Kamar AS, Shamsuri MH. Current status of Corynespora leaf fall in Malaysia. In: Proceeding workshop on Corynespora leaf fall disease of Hevea rubber, 16–17 December 1996, Medan, Indonesia. p. 21–8.

  18. Ismail H, Jeyanayagi I. Occurrence and identification of physiological races of Corynespora cassiicola of Hevea. In: Proceeding of IRRDB Symposium 1999. Hainan Publishing House. p. 263–72.

  19. Darmono TW, Darussamin A, Pawirosoemardjo S. Variation among isolates of Corynespora cassiicola associated with Hevea brasiliensis in Indonesia. In: Proceeding workshop on Corynespora leaf fall disease of Hevea rubber, 16–17 December 1996, Medan, Indonesia. p. 79–91.

  20. Silva WPK, Karunanayake EH, Wijesundera RLC, Priyanka UMS. Genetic variation in Corynespora cassiicola: a possible relationship between host origin and virulence. Mycol Res. 2003;107(5):567–71. doi:10.1017/S0953756203007755.

    Article  PubMed  CAS  Google Scholar 

  21. Saha T, Arun Kumar A, Sreena S, Joseph A, Kuruvilla Jacob C, Kothandaraman R, Nazeer MA. Genetic variability of Corynespora cassiicola infecting Hevea brasiliensis isolated from the traditional rubber growing areas in India. Indian J Nat Rubber Res. 2000;13(1&2):1–10.

    Google Scholar 

  22. Atan S, Hamid NH. Differentiating races of Corynespora cassiicola using RAPD and internal transcribed spacer markers. J Rubber Res. 2003;6(1):58–64.

    CAS  Google Scholar 

  23. Romruensukharom P, Tragoonrung S, Vanavichit A, Toojinda T. Genetic variability of Corynespora cassiicola population in Thailand. J Rubber Res. 2005;8(1):38–49.

    Google Scholar 

  24. Othman R, Benong M, Ong SH, Ismail H. Strategies and development of resistant Hevea clones against Corynespora leaf fall. In: Proceeding workshop on Corynespora leaf fall disease of Hevea rubber, 16–17 December 1996, Medan, Indonesia. p. 177–94.

  25. RRIM Malaysian Country Report, IRRDB Workshop on Corynespora Leaf Fall of Rubber, 6–9 June 2000, Malaysia and Indonesia 2000.

  26. Doyle JJ, Doyle JL. Isolation of plant DNA from fresh tissue. Focus. 1990;12:13–5.

    Google Scholar 

  27. Philip S, Roy B. Molecular characterization of Corynespora cassiicola. In: A laboratory manual for international training on strategies for management of Corynespora leaf fall disease of H. brasiliensis. 18–29 April 2006, Rubber Research Institute of India. p. 8–16.

  28. Mishra PK, Tewari JP, Clear RM, Turkington TK. Molecular genetic variation and geographical structuring in Fusarium graminearum. Ann Appl Biol. 2004;145:299–307. doi:10.1111/j.1744-7348.2004.tb00387.x.

    Article  CAS  Google Scholar 

  29. Nei M, Li WH. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc Natl Acad Sci USA. 1979;76:5269–73. doi:10.1073/pnas.76.10.5269.

  30. Pavlíček A, Hrdá Š, Flegr J. FreeTree––Treeware program for construction of phylogenetic trees on the basis of distance data and for bootstrap/jackknife analysis of the trees robustness. Application in the RAPD analysis of genus Frenkelia. Folia Biologica (Praha). 1999;45:97–9.

    Google Scholar 

  31. Page RDM. TREEVIEW: an application to display phylogenetic trees on personal computers. Comput Appl Biosci. 1996;12:357–8.

    PubMed  CAS  Google Scholar 

  32. Liyanage AS, Jayasinghe CK, Liyanage NIS, Jayaratne AHR. Corynespora leaf spot disease of rubber (Hevea brasiliensis)––A new report. J Rubber Res Inst Sri Lanka. 1986;65:47–50.

    Google Scholar 

  33. Chee KH. Studies on sporulation, pathogenicity and epidemiology of Corynespora. J Nat Rubber Res. 1988;1(3):21–9.

    Google Scholar 

  34. Ellis MB, Holliday P. Corynespora cassiicola. C. M. I. description of pathogenic fungi and bacteria. No. 303. 1971.

  35. Mushrif SK. Morphology, physiology and survival of Corynespora cassiicola (Bert.&Curt.) Wei. In: K Jacob, editor. Corynespora leaf disease of Hevea brasiliensis. Strategy for management. Rubber Research Institute of India; 2006. p. 26–32.

  36. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. Basic local alignment search tool. J Mol Biol. 1990;215(3):403–10.

    PubMed  CAS  Google Scholar 

  37. Spencer JA, Walter HJ. Variation in certain isolates of Corynespora cassiicola. Phytopathology. 1969;59:58–60.

    Google Scholar 

  38. Onesirosan PT, Arny DC, Durbin RD. Host specificity of Nigerian and North American isolates of Corynespora cassiicola. Phytopathology. 1974;64:1364–7.

    Google Scholar 

  39. Duarte MLR, Asano S, Albuquerque FC. Comparative study of the morphological and physical characteristics of two Corynespora cassiicola isolates. Fitopatologia Brasileira. 1983;8:205–14.

    Google Scholar 

  40. Dung Phan Thanh. Studies on Corynespora cassiicola (Bert. & Curt.) Wei on Rubber. Master Thesis, Universiti Pertanian Malaysia (UPM) 1995.

  41. Silva WPK, Deverall BJ, Lyon BR. Molecular, physiological and pathological characterization of Corynespora leaf spot fungi from rubber plantations in Sri Lanka. Plant Pathol. 1998;47(3):267–77. doi:10.1046/j.1365-3059.1998.00245.x.

    Article  CAS  Google Scholar 

  42. Gil-Lamaignere C, Roilides E, Hacker J, Muller FMC. Molecular typing for fungi––a critical review of the possibilities and limitation of currently and future methods. Clin Microbiol Infect 2003;9:172–85. doi:10.1046/j.1469-0691.2003.00649.x.

    Google Scholar 

  43. International Atomic Energy Agency. Mutant germplasm characterization using molecular markers: a Manual, IAEA-TCS-19, IAEA. 2002; Introduction 11.

  44. Zietkiewicz E, Rafalski A, Labuda D. Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification. Genomics. 1994;20:176–83. doi:10.1006/geno.1994.1151.

    Article  PubMed  CAS  Google Scholar 

  45. Goldwin ID, Aitken AB, Smith LW. Application of Inter simple sequence repeats (ISSR) markers to plant genetics. Electrophoresis. 1997;18:1524–8. doi:10.1002/elps.1150180906.

    Article  Google Scholar 

  46. Burgess T, Wingfield MJ, Wingfield BW. Simple sequence repeat markers distinguish among morphotypes of Sphaeropsis sapinea. Appl Environ Microbiol. 2001;67(1):354–62. doi:10.1128/AEM.67.1.354-362.2001.

    Article  PubMed  CAS  Google Scholar 

  47. Meng X, Chen W. Applications of AFLP and ISSR techniques in detecting genetic diversity in the soybean brown stem rot pathogen Phialophora gregata. Mycol Res. 2001;105(8):936–40. doi:10.1016/S0953-7562(08)61949-8.

    Article  CAS  Google Scholar 

  48. Van Der Merwe NA, Wingfield BD, Wingfield MJ. Primers for the amplification of sequence-characterized loci in Cryphonectria cubensis populations. Mol Ecol Notes. 2003;3:494–7. doi:10.1046/j.1471-8286.2003.00508.x.

    Article  Google Scholar 

  49. Kauserud H, Schumacher T. Regional and local population structure of the pioneer wood-decay fungus Trichaptum abietinum. Mycologia. 2003;95(3):416–25.

    Article  CAS  Google Scholar 

  50. Menzies JG, Bakkeren G, Matheson F, Procunier JD, Woods S. Use of inter-simple sequence repeats and amplified fragment length polymorphisms to analyze genetic relationships among small grain-infecting species of Ustilago. Phytopathology. 2003;93:167–75. doi:10.1094/PHYTO.2003.93.2.167.

    Article  CAS  Google Scholar 

  51. Kauserud H. Widespread vegetative compatibility groups in the dry-rot fungus Serpula lacrymans. Mycologia. 2003;96(2):232–9. doi:10.2307/3762059.

    Article  Google Scholar 

  52. Elena Estrada M, Camacho MV, Benito C. The molecular diversity of different isolates of Beauveria Bassiana (Bals.) Vuill. As assessed using intermicrosatellites (ISSRs). Cell Mol Biol Lett. 2007;12:240–52. doi:10.2478/s11658-006-0069-4.

    Article  PubMed  Google Scholar 

Download references

Acknowledgements

The study presented in this paper was undertaken as part of a doctoral study project which was supported by Universiti Putra Malaysia (UPM) and Malaysian Rubber Board (MRB). Nguyen Anh Nghia, a research officer from Rubber Research Institute of Vietnam (RRIV), was financially sponsored by a Scholarship from the Ministry of Education, Vietnam Government. The authors would like to thank Dr. Shamsul Bahari from the MRB for granting permission to use the facilities for conidial morphology study.

Author information

Authors and Affiliations

  1. Rubber Research Institute of Vietnam, 177 Hai Ba Trung St., District 3, Ho Chi Minh City, Vietnam

    Nguyen Anh Nghia

  2. Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400, UPM Serdang, Selangor, Malaysia

    Nguyen Anh Nghia, Mohd. Puad Abdullah & Suhaimi Napis

  3. Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, 43400, UPM Serdang, Selangor, Malaysia

    Jugah Kadir

  4. RRIM, Malaysian Rubber Board, 47000, Sungai Buloh, Selangor, Malaysia

    E. Sunderasan & Adam Malik

Authors
  1. Nguyen Anh Nghia
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jugah Kadir
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. E. Sunderasan
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Mohd. Puad Abdullah
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Adam Malik
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Suhaimi Napis
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Suhaimi Napis.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Nghia, N.A., Kadir, J., Sunderasan, E. et al. Morphological and Inter Simple Sequence Repeat (ISSR) Markers Analyses of Corynespora cassiicola Isolates from Rubber Plantations in Malaysia. Mycopathologia 166, 189–201 (2008). https://doi.org/10.1007/s11046-008-9138-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11046-008-9138-8

Keywords

Search

Navigation