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Antennal transcriptome analysis of Psyttalia incisi (silvestri) (Hymenoptera: Braconidae): identification and tissue expression profiling of candidate odorant-binding protein genes

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Abstract

Background

Olfaction plays an important role in host-seeking by parasitoids, as they can sense chemical signals using sensitive chemosensory systems. Psyttalia incisi (Silvestri) (Hymenoptera: Braconidae) is the dominant parasitoid of Bactrocera dorsalis (Hendel) in fruit-producing regions of southern China. The olfactory behavior of P. incisi has been extensively studied; however, the chemosensory mechanisms of this species are not fully understood.

Results

Bioinformatics analysis of 64,515 unigenes from the antennal transcriptome of both male and female adults P. incisi identified 87 candidate chemosensory genes. These included 13 odorant-binding proteins (OBPs), seven gustatory receptors (GRs), 55 odorant receptors (ORs), 10 ionotropic receptors (IRs), and two sensory neuron membrane proteins (SNMPs). Phylogenetic trees were constructed to predict evolutionary relationships between these chemosensory genes in hymenopterans. Moreover, the tissue expression profiles of 13 OBPs were analyzed by quantitative real-time PCR, revealing high expression of seven OBPs (1, 3, 6, 7, 8, 12, and 13) in the antennae.

Conclusion

This study represents the first identification of chemosensory genes and the determination of their expression patterns in different tissues of P. incisi. These results contribute to a better understanding of the function of the chemosensory system of this parasitoid species.

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Data availability

The authors confirm that the data supporting the findings of this study are available within the article and its supplementary materials.

Abbreviations

OBPs:

Odorant-binding proteins

GRs:

Gustatory receptors

ORs:

Odorant receptors

IRs:

Ionotropic receptors

SNMPs:

Sensory neuron membrane proteins

CSPs:

Chemosensory proteins

PBPs:

Pheromone-binding proteins

GOBPs:

General odorant-binding proteins

NCBI:

NR-NCBI non-redundant

NCBI:

NT-NCBI nucleotide

GO:

Gene Ontology

KEGG:

Kyoto Encyclopedia of Genes and Genomes

Pfam:

Protein Family

KOG/COG:

EuKaryotic Ortholog Groups/Clusters of Orthologous Groups

ORFs:

Open reading frames

TMDs:

Transmembrane domains

pI:

Theoretical isoelectric point

Mw:

Molecular weight

TMDs:

Transmembrane domains

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Acknowledgements

We thank Ms. Shumei Wang, Institute of Biological Control, Fujian Agriculture and Forestry University, Fuzhou, China, for kindly rearing and providing parasitoids and fruit flies.

Funding

This research was supported by the advanced Talents Introduction Project of Wuyi University (YJ201910); the Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, Fujian Agriculture and Forestry University (Keylab2020-02, Keylab2021-05); Key Project of Nanping Natural Science Foundation (N2023J004); Key Technological Innovation and Industrialization Project (2023XQ019); Special Funds for Technological Representative (NP2021KTS04).

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  1. Deqing Yang and Dongliang Li contributed equally to this work.

Authors and Affiliations

  1. Institute of Biological Control, Plant Protection College, Fujian Agriculture and Forestry University, Fuzhou, China

    Deqing Yang, Lili Jiang, Jia Lin, Guoqing Yue, Kang Xiao, Xuxing Hao, Qinge Ji, Pumo Cai & Jianquan Yang

  2. Key Laboratory of Biopesticide and Chemical Biology, Institute of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, China

    Deqing Yang, Lili Jiang, Jia Lin, Guoqing Yue, Kang Xiao, Xuxing Hao, Qinge Ji, Pumo Cai & Jianquan Yang

  3. State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Institute of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, China

    Deqing Yang, Lili Jiang, Jia Lin, Guoqing Yue, Kang Xiao, Xuxing Hao, Qinge Ji, Pumo Cai & Jianquan Yang

  4. College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou, Fujian Province, China

    Dongliang Li

  5. Department of Horticulture, College of Tea and Food Science, Wuyi University, Wuyishan, China

    Deqing Yang, Dongliang Li, Yongcong Hong & Pumo Cai

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  1. Deqing Yang
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Contributions

Methodology, D.Y., Q.J., J.Y. and P.C.; performed the experiments, D.Y., L.J., G.Y., X.H. and K.X.; analyzed the data, D.Y., L.J., Y.H. and D.L.; drafted the manuscript, D.Y. and P.C.; revised the manuscript, D.Y., J.Y. and P.C. All the authors read and approved the publication. All authors have read and agreed to the published version of the manuscript.

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Correspondence to Pumo Cai or Jianquan Yang.

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11033_2024_9281_MOESM1_ESM.docx

Supplementary Material 1: Table S1: Model of phylogentic trees; Table S2: Primers for qRT-PCR of PincOBP genes in P. incisi; Table S3: Summary of P. incisi antennae transcriptome; Table S4: Gene annotation ratio; Table S5: BLASTx alignment of gustatory receptor genes of Psyttalia incisi and other insect species; Table S6: BLASTx alignment of odorant receptor genes of Psyttalia incisi and other insect species; Table S7: BLASTx alignment of ionotropic receptor (IR) genes of Psyttalia incisi and other insect species; Table S8: BLASTx alignment of sensory neuron membrane protein (SNMP) genes of Psyttalia incisi and other insect species. Figure S1: Distribution of unigene size in the P. incisi transcriptome assembly; Figure S2: Pie chart of homologous species distributionin in NCBI-NR database; Figure S3: Gene ontology (GO) classification of unigenes; Figure S4: KEGG classification of P. incisi unigenes; Figure S5: KOG classification of P. incisi unigenes.

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Yang, D., Li, D., Jiang, L. et al. Antennal transcriptome analysis of Psyttalia incisi (silvestri) (Hymenoptera: Braconidae): identification and tissue expression profiling of candidate odorant-binding protein genes. Mol Biol Rep 51, 333 (2024). https://doi.org/10.1007/s11033-024-09281-3

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