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The acetyltransferase BmCBP changes the acetylation modification of BmSP3 and affects its protein expression in silkworm, Bombyx mori

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Abstract

Background

Protein acetylation is an important post-translational modification (PTM) that widely exists in organisms. As a reversible PTM, acetylation modification can regulate the function of proteins with high efficiency. In the previous study, the acetylation sites of silkworm proteins were identified on a large scale by nano-HPLC/MS/MS (nanoscale high performance liquid chromatography-tandem secondary mass spectrometry), and a total of 11 acetylation sites were discovered on Bombyx mori nutrient-storage protein SP3 (BmSP3). The purpose of this study was to investigate the effect of acetylation level on BmSP3.

Methods and results

In this study, the acetylation of BmSP3 was further verified by immunoprecipitation (IP) and Western blotting. Then, it was confirmed that acetylation could up-regulate the expression of BmSP3 by improving its protein stability in BmN cells. Co-IP and RNAi experiments showed acetyltransferase BmCBP could bind to BmSP3 and catalyze its acetylation modification, then regulate the expression of BmSP3. Furthermore, the knock-down of BmCBP could improve the ubiquitination level of BmSP3. Both acetylation and ubiquitination occur on the side chain of lysine residues, therefore, we speculated that the acetylation of BmSP3 catalyzed by BmCBP could competitively inhibit its ubiquitination modification and improve its protein stability by inhibiting ubiquitin-mediated proteasome degradation pathway, and thereby increase the expression and intracellular accumulation.

Conclusions

BmCBP catalyzes the acetylation of BmSP3 and may improve the stability of BmSP3 by competitive ubiquitination. This conclusion provides a new functional basis for the extensive involvement of acetylation in the regulation of nutrient storage and utilization in silkworm, Bombyx mori.

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Funding

This work was supported by financial grants from the Natural Science Foundation of Zhejiang Provice (No. LY20C170002) and the National Natural Science Foundation of China (NO. 31772677).

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Author notes

  1. The authors Guowei Zu and Zihan Sun have contributed equally to this work.

Authors and Affiliations

  1. College of Life Sciences and Medicine, Zhejiang provincial key laboratory of Silkworm Bioreactor and Biomedicine, Zhejiang Sci-Tech University, 310018, Hangzhou, China

    Guowei Zu, Zihan Sun, Yanmei Chen, Jiasheng Geng, Jiao Lv, Zhengying You, Caiying Jiang, Qing Sheng & Zuoming Nie

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  1. Guowei Zu
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  2. Zihan Sun
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  3. Yanmei Chen
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Contributions

All authors contributed to the study conception and design. Review and editing by [Guowei Zu] and [Zuoming Nie], methodology by [Guowei Zu], [Zihan Sun], [Yanmei Chen], [Jiasheng Geng], [Jiao Lv], and [Zuoming Nie], Conceptualization by [Zhengying You], [Caiying Jiang], [Qing Sheng], The first draft of the manuscript was written by [Guowei Zu] and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Zuoming Nie.

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Zu, G., Sun, Z., Chen, Y. et al. The acetyltransferase BmCBP changes the acetylation modification of BmSP3 and affects its protein expression in silkworm, Bombyx mori. Mol Biol Rep 50, 8509–8521 (2023). https://doi.org/10.1007/s11033-023-08699-5

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