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Complete genome sequence of a novel bacteriophage vB_Pci_PCMW57 infecting phytobacteria pseudomonas cichorii

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Abstract

Background

A novel virulent bacteriophage infecting phytobacteria Pseudomonas cichorii (P. cichorii) was isolated from leafy vegetables in Brazil. P. cichorii is a Gram-negative soil phytobacterium, the causal agent of a number of economically important plant diseases worldwide.

Methods and results

In this study, a new phage specific for P. cichorii was isolated from solid samples (lettuce, chicory and cabbage), designated vB_Pci_PCMW57. Electron microscopy revealed a small virion (~ 50-nm-diameter icosahedral capsid) with a short, non-contractile tail. The genome of vB_Pci_PCMW57 is 40,117 bp in size, with a GC content of 57.6% and encodes 49 open reading frames. The phage is genetically similar to P. syringae phages Pst_GM1 and Pst_GIL1, and the P. fluorescens phages WRT and KNP. According to electron microscopy and whole-genome sequence analysis, vB_Pci_PCMW57 should be classified as a Caudoviticetes, family Autographiviridae, subfamily Studiervirinae.

Conclusions

The complete phage genome was annotated, and the sequence identity of the virus with other Pseudomonas viruses was higher than 95%. To our knowledge, this is the first report of a bacteriophage infecting Pseudomonas cichorii.

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Funding

Author Alves MK has received a grant from CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Brazil).

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Authors and Affiliations

  1. Instituto de Biotecnologia, Universidade de Caxias do Sul, Caxias do Sul, RS, Brazil

    Márcia Keller Alves, Wellington Vieira de Souza, João Antonio Pêgas Henriques & Mariana Roesch Ely

  2. Universidade de Caxias do Sul, Caxias do Sul, RS, Brazil

    Júnia Capua de Lima Novello

  3. INL – International Iberian Nanotechnology Laboratory, Av. Mestre José Veiga, Braga, 4715-330, Portugal

    Sanna Sillankorva

  4. Applied Magnetism Laboratory, Brazilian Center for Research in Physics, Rio de Janeiro, RJ, Brazil

    Cilene Labre & Rubem Luís Sommer

Authors
  1. Márcia Keller Alves
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  2. Wellington Vieira de Souza
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  3. Júnia Capua de Lima Novello
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  4. Sanna Sillankorva
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  5. Cilene Labre
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  6. Rubem Luís Sommer
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  7. João Antonio Pêgas Henriques
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  8. Mariana Roesch Ely
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Contributions

Alves MK designed the study, isolated and purified bacteriophages, isolated the phage DNA, wrote the manuscript. Souza WV performed the research, isolated and purified bacteriophages, isolated the phage DNA. Novello JCL contributed to the design of the work, isolated and purified bacteriophages, contributed new methods. Sillankorva S contributed to the design of the work, contributed new methods, performed the phage genome sequencing and bioinformatic analyses, analyzed the data. Labre C and Sommer RL perfomed transmission electron microscopy. Henriques JAP made substantial contributions to the design of the work, revised it critically. Ely MR made substantial contributions to the design of the work, revised it critically. All authors have read and approved the version to be published.

Corresponding author

Correspondence to Márcia Keller Alves.

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The authors have no relevant financial or non-financial interests to disclose.

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Alves, M.K., de Souza, W., Novello, J.C.L. et al. Complete genome sequence of a novel bacteriophage vB_Pci_PCMW57 infecting phytobacteria pseudomonas cichorii. Mol Biol Rep 50, 7105–7111 (2023). https://doi.org/10.1007/s11033-023-08552-9

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  • DOI: https://doi.org/10.1007/s11033-023-08552-9

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