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Marker assisted backcross to introgress late leaf spot and rust resistance in groundnut (Arachis hypogaea L.)

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Abstract

Background

Groundnut is affected by a variety of abiotic and biotic stressors, including late leaf spot and rust, which cause significant economic loss. In this study, QTL for resistance to late leaf spot and rust from donor variety GPBD 4 were incorporated into a popular groundnut variety (ICGV 00350) through marker assisted backcross (MABC) breeding.

Methods

Eight foreground SSR markers [AhXII (GM1009, GM1573 and Seq8D09) and AhXV (GM1536, GM2009, GM2079, GM2301 and IPAHM103)] linked with disease resistant QTLs were utilized in this study. A set of 217 SSR markers spanning the whole groundnut genome were employed for background analysis. Three backcrosses with recurrent parent and selfing were followed in the cross ICGV 00350 × GPBD 4. Background analysis was carried out in BC3F2; while, phenotypic confirmation for resistance was carried out in BC3F3 generation.

Conclusion

Five advanced backcross lines in BC3F2 were found, with more than 90% recurrent parent genome. The phenotyping of the eight ILs recorded disease scores ranging from 2.0 to 3.0 for LLS and from 1.0 to 3.0 for rust disease scores. All these lines had superior characteristics compared to the recurrent parent ICGV 00350 in terms of late leaf spot and rust resistance. The enhanced ILs will be evaluated further for commercial release.

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Funding

Authors are thankful to the Department of Biotechnology (DBT), Government of India, New Delhi for financial support to undertake this study (Grant No: BT/ PR6583/AGII /106/881/2012).

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Authors and Affiliations

  1. School of Post Graduate Studies, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, 641 003, India

    Prabhu Rajarathinam

  2. Department of Oilseeds, Centre for Plant Breeding and Genetics, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, 641 003, India

    Gopikrishnan Palanisamy

  3. National Pulses Research Centre, Tamil Nadu Agricultural University, Vamban, Pudukkottai, Tamil Nadu, 622 303, India

    Ramakrishnan P

  4. Centre for Plant Breeding and Genetics, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, 641 003, India

    Manivannan Narayana

  5. Agricultural College and Research Institute, Tamil Nadu Agricultural University, Vazhavachanur, Tamil Nadu, 606753, India

    Mothilal Alagirisamy

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Correspondence to Manivannan Narayana.

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Rajarathinam, P., Palanisamy, G., P, R. et al. Marker assisted backcross to introgress late leaf spot and rust resistance in groundnut (Arachis hypogaea L.). Mol Biol Rep 50, 2411–2419 (2023). https://doi.org/10.1007/s11033-022-08234-y

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