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Association between TCF7L2 polymorphism and type 2 diabetes mellitus susceptibility: a case–control study among the Bangladeshi population

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Abstract

Background

Diabetes is a severe health burden for Bangladesh. Genetic polymorphism has been reported to be one of the major risk factors for diabetes in various studies. TCF7L2 (transcription factor 7 like 2) transcripts in the human β-cell have effects on β-cell survival, function, and Wnt signaling activation. This study aimed to evaluate the frequency and association of various polymorphisms namely TCF7L2 rs12255372 and rs7903146 among Bangladeshi patients with T2DM (Type 2 Diabetes Mellitus).

Methods

This case–control study included 300 patients with T2DM and 234 healthy individuals from two health facilities in the Chattogram Division of Bangladesh. Anthropometric measurements were assessed using a self-reported, structured, eight-item questionnaire. The polymorphisms were identified by PCR–RFLP and sequencing method.

Results

A strong association of T2DM with polymorphisms was observed, including rs12255372 (p = 0.0004) and rs7903146 (p = 0.005). It was observed that the risk genotype at rs12255372 was associated with age (p = 0.009), a family history of diabetes (p < 0.0001), and HbA1C (p < 0.0001). Furthermore, it was found that rs12255372 was substantially associated with hypertension (p = 0.03), eye problems (p = 0.01), and neurological abnormalities (p = 0.02).

Conclusion

This study postulates that TCF7L2 genetic polymorphism is associated with the risk of T2DM among the studied Bangladeshi population. The findings should be replicated through more studies with a large number of samples and in different populations.

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Acknowledgements

Authors would like to thank all the volunteers of Disease Biology and Molecular Epidemiology Research group (dBme), Chittagong for their support.

Funding

This work was funded Grant from Research and publication office, University of Chittagong.

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Authors and Affiliations

  1. Department of Genetic Engineering & Biotechnology, Faculty of Biological Sciences, University of Chittagong, Chattogram, 4331, Bangladesh

    Asma Salauddin, Kallyan Chakma, Md. Mahbub Hasan & Adnan Mannan

  2. Department of Endocrinology, Chittagong Medical College, Chattogram, 4203, Bangladesh

    Farhana Akter

  3. Chittagong Diabetic General Hospital, Chattogram, Bangladesh

    Nowshad Asgar Chowdhury & Sumon Rahman Chowdhury

  4. Disease Biology and Molecular Epidemiology Research Group, Chattogram, Bangladesh

    Asma Salauddin, Kallyan Chakma, Md. Mahbub Hasan, Farhana Akter & Adnan Mannan

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  1. Asma Salauddin
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Contributions

Conceptualization—AM; Method development – Writing—Original Draft Preparation, AS, KC and AM.; Writing—Review & Editing, MMH., FA., NAC., SRC and AM.; All authors have read and agreed to the published version of the manuscript.

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Correspondence to Adnan Mannan.

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This study was approved by the Ethical Review Committee of Chittagong Medical College Hospital (CMC/PG/2019/57).

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Salauddin, A., Chakma, K., Hasan, M.M. et al. Association between TCF7L2 polymorphism and type 2 diabetes mellitus susceptibility: a case–control study among the Bangladeshi population. Mol Biol Rep 50, 609–619 (2023). https://doi.org/10.1007/s11033-022-08081-x

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