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HPV 16 E6 promotes growth and metastasis of esophageal squamous cell carcinoma cells in vitro

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Abstract

Background

Esophageal squamous cell carcinoma (ESCC) is one of the most lethal malignancies worldwide. Increasing evidence suggests that human papillomavirus (HPV) infection may be associated with the etiology of ESCC. However, the precise role of HPV in ESCC remains unclear.

Methods and results

Proliferation and apoptosis of ESCC cells upon infection with HPV16 E6 were detected using CCK-8 assays and Western blot analyses. The migration rate was measured with a wound healing assay, and a Transwell Matrigel invasion assay was used to detect the invasive ability. RT-qPCR was performed to detect the expression of E6AP, p53, and miR-34a. The proliferation rates were significantly higher in HPV16E6-transfected cell groups compared with the negative control groups. Bax protein expression was downregulated in HPV16E6-treated groups compared to the controls. The wound healing and Transwell Matrigel invasion assays indicated that HPV16 E6 infection could increase ESCC cell migration and invasion. Furthermore, E6AP, p53 and miR-34a expression were decreased in HPV16 E6-transfected cell lines.

Conclusion

Our results not only provide evidence that HPV16 E6 promotes cell proliferation, migration, and invasion in ESCC, but also suggests a correlation between HPV infection and E6AP, p53 and miR-34a expression. Consequently, HPV16 E6 may play an important role in ESCC development.

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Data availability

The datasets obtained and/or analysed during the current study are available from the corresponding author on reasonable request.

Code availability

Not applicable.

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Acknowledgements

We thank the Chinese Academy of Medical Sciences and Fuxiang biotechnology of shanghai for providing cell lines.

Funding

This work was supported by the National Natural Science Foundation of China (No. 81860518, No. 81260301).

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Author notes

  1. JiaoJiao Hu and Yu Ji have contributed equally to this work.

Authors and Affiliations

  1. Department of Pathology, Shihezi University School of Medicine, 832000, Shihezi, Xinjiang, People’s Republic of China

    JiaoJiao Hu, Yu Ji, TingTing Miao, ShiYao Zheng, XiaoBin Cui, JianMing Hu, Lan Yang & Feng Li

  2. Department of Pathology, The First Affiliated Hospital of Shihezi University School of Medicine, Shihezi University School of Medicine, 832000, Shihezi, Xinjiang, China

    ShiYao Zheng, XiaoBin Cui, JianMing Hu, Lan Yang & Feng Li

  3. Department of Pathology and Medical Research Center, Beijing Chaoyang Hospital, Capital Medical University, 100020, Beijing, People’s Republic of China

    Feng Li

  4. Pathology Department, Jiangmen Maternity and Child Health Care Hospital, Guangdong, 529000, Jiangmen, People’s Republic of China

    Yu Ji

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  1. JiaoJiao Hu
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Contributions

YL, XBC, JMH and FL conceived of and supervised this study. JJH, YJ and TTM performed experiments. SYZ analysed the data. JJH, and YJ wrote the manuscript with input from all authors.

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Correspondence to Lan Yang or Feng Li.

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Hu, J., Ji, Y., Miao, T. et al. HPV 16 E6 promotes growth and metastasis of esophageal squamous cell carcinoma cells in vitro. Mol Biol Rep 50, 1181–1190 (2023). https://doi.org/10.1007/s11033-022-07952-7

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